TP53 Gene Status in the Presence of Allicin in NNK Induced Pancreatic Cancer Albino Rats


  • Phillip Abutu Paelon Memorial Hospital, Plot 1221, Ahmadu Bellow way Victoria Island, Lagos, 101241, Nigeria
  • Joseph Minari University of Lagos, Akoka, yaba, Lagos, 100213, Nigeria
  • Gbadebo Oyeniyi General Hospital, Offa, Kwara State, Address, Offa and 250101, Nigeria
  • Prince Ugwu College of Medicine, University of Ibadan, Chemical Pathology Department, Ibadan, 200212, Nigeria
  • Juliet Obi Department of Biomedical Sciences, Ladoke Akintola University, Osogbo, 210214, Nigeria
  • Anthony Okorie Department of Medical Laboratory Science, Faculty of Health Sciences, Nnamdi Azikwe University,420110, Nigeria
  • Titilola Okwoli Department of Medical Laboratory, St Nicholas Hospital, Lagos, 101241, Nigeria
  • Oluwaseun Abutu Pediatric Partners, Akin Ogunlewe, Victoria Island, Lagos,101241, Nigeria


NNK, HPLC, DNA, Allicine, PC.


Pancreatic cancer (PC) is the fourth most lethal form of cancer in Western society, with mortalities closely mirroring incidence and an overall 5-year survival of less than 7%. Traditional medicine which involves the use of herbs has been used to treat various types of cancer and this has been found to be effective with minimal or no side effects. This ressearch was aimed at evaluating the chemo preventive effect of extract of the active ingredients of garlic (Allicine)on Nitrosamines induced pancreatic cancer in rat. Allicine was extracted from garlic by crushing the garlic in an ethanol and inject  into the HPLC column. Agarose gel electrophoresis was used to analyze deoxyribonuleic acid (DNAs) extracted from the pancreatic tissue of the experimental mice while haematoxylin and eosin staining was used for histological assay.

There was a significant reduction in the weight of the experimental animals that were administered with NNK. The animals administered with extract (allicin) also show a reduction in weight. Normal control animals were seen to have gained weight. DNA bands obtained from agarose gel electrophoresis suggested a possible NNK induced damage which was prevented to a certain degree owing to the effect of the allicin. Histological assay revealed the presence of neoplastic hyperplasia which is more prominent in the histological sections of the pancreatic tissue of the rats which were not treated with allicin. This study has shown that allicin could be used as a treatment against NNK induced pancreatic cancer in white albino rats at the right concentration.


. Adebamowo, C.A. and Ajayi, O.O. (2000). Breast cancer in Nigeria. West African Journal Medicine 19,179-194.

. Alawode, T. T. 2013. An overview of the anti-cancer properties of some plants used in traditional medicine in Nigeria. International Research Journal of Biochemistry and Bioinformatics. 3(1): 7-14.

. America Cancer Society. 2007 Cancer Facts and Figures. Atlanta, GA: America Cancer

. American Cancer Society. 2012. Breast Cancer facts and figures. American Cancer Society,

. American Cancer Society. 2014. Key Statistics about Cancer

. American Cancer Society. 2015. Cancer Facts and Figures. Atlanta, Ga: American Cancer

. Awodele, O., Adeyomoye, A.A., Awodele, D.F., Fayankinu, V.B and Dolapo, D.C. (2011). Cancer distribution pattern in south-western Nigeria. Tanzanian Journal of Health Reseach. 13(2): 1-8.

. Bond-Smith, G., Banga N., Hammond T. M. 2012. Imber Clinical Journal. 344: 2476 -2477. evaluation and anticancer screening of rhizomes of Kaempferia galangal Linn. International Research Journal of Pharmacy. 9:1-2.

. Hruban, R. H, Petersen, P. K. Ha, S.E. 1998. Kern Genetics of pancreatic cancer from genes International Atlanta.

. Jemal, A., 2008. Cancer statistics, 2008. Cancer Journal of Clinic. 58:71–96.

. Mao, J. J, Palmer, C. S., Healy, K. E., Desai, K and Amsterdam, J (2011). Complementary and alternative medicine use among cancer survivors: a population-based study. Journal of Cancer Survivorship. 5: 8-17.

. Mathew, B., Vijayabaskarb, M., Mathewa, G.E., Thomas, K.M (2010). Phytochemical

. Michaud D.S, Giovannucci E, Willett W.C, Colditz G.A, Stampfer M.J, Fuchs C.S (2001). Physical activity, obesity, height, and the risk of pancreatic cancer. JAMA. 286:921-9.

. Michaud, D.S., Skinner, H.G., Wu, K., Hu, K., Giovannucci, E., Willett, W.C., Colditz, G.A., and Fuchs, C.S (2005). Dietary patterns and pancreatic cancer risk in men and women. Journal of National Cancer Institute. 97:518-524.

. National Cancer Institute. 2014. Defining Cancer. Reviewed 10 June 2014. of families. Surgery oncology of clinical journal. 7 (1) 1-23.

. Ozbay, T. and Nahta, R (2011). Delphinidin inhibits HER2 and Erk1/2 signalling and suppresses growth of HER2 overexpressing and triple negative breast cancer cell lines. Basic and Clinical Research. 5: 143-154.

. Simonetti, G. (1990). Schuler, S., ed. Simon & Schuster's Guide to Herbs and Spices. Simon and Schuster, Inc Society.

. Sofowora, A. (2008). Medicinal plants and traditional medicine in Africa. John Wiley and sons LTD, Pp.1-10.

. Sowemimo, A., Van de4. Venter M., Baatjies L, and Koekemoer T (2011). Cytotoxicity evaluation of selected Nigerian plants used in traditional cancer treatment. Journal of Medicinal Plants Research. 5(11): 2442-244.




How to Cite

Abutu, P., Minari, J., Oyeniyi, G., Ugwu, P., Obi, J., Okorie, A., Okwoli, T., & Abutu, O. (2019). TP53 Gene Status in the Presence of Allicin in NNK Induced Pancreatic Cancer Albino Rats. International Journal of Sciences: Basic and Applied Research (IJSBAR), 46(1), 168–182. Retrieved from