Anti-hyperglycemic Activity in Vitro, Actuate Toxicity in Vivo and Antioxidant Activity of the Crude Extract of the Root of Plumeria alba L.

Abstract

Medicinal plants are the single most productive sources for the development of drugs and play an important role in treating and preventing a variety of diseases through the world.  Plumeria alba Linn. commonly known as Tayoke-sakar-aphyu in Myanmar is one of the medicinal plants belonging to Apocynaceae family. The pharmacological studies were carried out to investigate antimicrobial activity, antioxidant activity, anti-hyperglycemic activity in vitro and acute toxicity in vivo. The main aim of the present research is to evaluate the biological activities of the root of Plumeria alba L, Firstly, phytochemical screening of the collected sample was performed. Elemental composition of the crude sample was examined by EDXRF (Energy Dispersive X-ray Fluorescence) spectroscopy. Moreover three different solvents such as ethanol, ethyl acetate and n-hexane extracts of the sample were examined for their antimicrobial activities against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and E. coli by Agar- well diffusion method. The antioxidant activity of ethanol extract of the root of Plumeria alba L. was studied by DPPH (1,1-Diphenyl-2-picryl-hydrayl) assay. The acute toxicity of ethanol extract of the root of Plumeria alba L. on Artemia salinna were investigated. The glucose lowering activities of the water and ethanol extracts of the sample were determined by iodometric titration.