Studying of Oxidative Stress and Some Biochemical Parameters in Patients with ?-Thalassemia Major in Kirkuk City
Keywords:
Enzyme, Oxidative stress, β-thalassemia major, MDA, NO.Abstract
This research include studying the oxidative stress state and measuring some biochemical parameters in the blood of ?-thalassemia major which include: Acetylcholinesterase (AChE), Aspartate aminotransferase(AST), Alanine aminotransferase (ALT), Alkaline phosphatase (ALP),Catalase(CAT),Glutathione (GSH), Malonadialdehyde (MDA),Total bilirubin (Tb), Ceruloplasmin(Cp),Nitric oxid (NO) and Peroxynitrite(ONOO-). The study was done on (80)thalassemic patients (42) were male , (38)female and (40) normal healthy subjects as control group (21) were male and (19)female ,the age of patients and control group ranged from 3-30 years for both sexes.The results showed that there was a significant increase in the activity of AChE, AST, ALT, ALP, Cp,ONOO-and Tb in ?-thalassemia major compared with healthy group for both sexes, these result were indicated that these parameters may be good biochemical markers for this disease .Beside of that, the results showed there was a highly oxidative stress in the patients for both sexes, so there was a significant increase in the activity of oxidant indicator of MDA, with a significant decrease in NO and GSH levels in thalassemic patients compared with healthy group for both sexes.
References
– Weatherall, D.J. ( 2010) . "The inherited diseases of hemoglobin are an emerging global health burden" J.; Blood, 115 .pp: 4331,36.
- Galanello R, Origa R. review.(2010) Beta-thalassaemia. Orphanet J Rare Dis5: 11-26.
- Nasiri, m.; Hosseini,S.H.;and shah Mohammadi,S.(2014).Mental health status in patiemts with Thalassemia major in Iran,Journal of Pediatrics Review office ,2 (1):55-56 .
- Borgna-pignati C. Galanello R.(2004). Thalassaemias and related disorders: Quantitative disorders of hemoglobin synthesis. In: Greer JP, Foerestor J, Lukens JN, eds. Wintrobes Clinical Hematology. 11th ed. Philadelphia: Lippincott Williams & Wilkins; P. 1319-65.
- Kohne, E. (2011): Hemoglobinopathies, Clinical Manifestations, Diagnosis, and Treatment. Dtsch Arztebl Int.; 108: 532–540.
- Michael B , Peter Eckl( 2015)." Introduction to Oxidative Stress in Biomedical and Biological ResearchBiomolecules", 5, 1169-1177.
- R. A. Ghone, K. M. Kumbar,A.N. Suryakar, R. V. Katkam, andN. G. Joshi,(2008). “Oxidative stress and disturbance in antioxidant balance in beta thalassemia major,” Indian Journal of Clinical Biochemistry, vol. 23, no. 4, pp. 337–340.
- Ayala, A., Muñoz, M. F, Argüelles, S.(2014). Lipid peroxidation: Production,metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014: 360438.
- Gupta, V.K, Pal,NJ. Siddiqi andB.Sharma,(2015).Acetylcholineserase From human erythrocytes as a surrogate biomarker of lead induced neurotoxicity .Enzyme Res, 10.1155/2015/370705.
- Altman, B.J., Stine, Z. E., Dang C.V. ,(2016). From Krebs to clinic: Glutamine metabolism to cancer therapy. Nature Reviews Cancer, 16(10).
- J. Kodydkov á, L; VávrovrováM. Koc ík; A. Žák.(2014).Human Catalase, Its Polymorphisms Regulation and Changes of Its Activity in Different Diseases. 4th Department of Internal Medicine,Charles University in Prague and General University Hospital, Prague, Czech Republic;60(4):153-167.
- Laurence Cole, Peter R. Kramer,(2016) in Human Physiology, Biochemistr and Basic Medicine, P( 227-236) .
- Raafat N, Safy UE, Khater N, Hassan T, Hassan B, Siam A, et al. (2014).Assessment of Cognitive Function in Children With Beta-Thalassemia Major: A Cross Sectional Study. J Child Neurol,1−6.
- Ellman G.L., Courtney, K.D., Andrews, Vjr, Featherstone R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol, 1961; 7:88-95.
-Reitman, S., Frankel, S. A colorimetric method for the determination of serum GOT and GPT. Amer. J. Clin. Path, 1957; 28: 56-63.
. Kind, P. R., King, E. J. Estimation of plasma phosphatase by determination of hydrolyzed phenol with amino-antipyrine. Journal Clin. Path., 1954; 7: 322-326.
- Guidet, B.; shah, S. V. (1989). Am. J. Physiol 257 (26). F440 cited by Muslih, R. K.; Al-Nimer, M.S; Al-Zamely, O.Y. (2002). "The level of malondialdehyde after activation with H2O2 and CuSO4 and inhibition by deferoxamine and Molsidomine in the serum of patient with acute Myocardial infraction". Nat. J. chem.; 5.p:139-148.
- Abi,H.(1974). Method of enzymatic analysis. New York Academic press ,2:674-684
– Sunderman, F. W.and Nomato, S. (1970)." Measurement of human serum ceruloplasmin by its para phenylendiamine oxidase activity" Clin. Chem.; 16(11).p: 903-910.
- Dervisevic,A.,Babic,N.,Huskic,J.,Sokolovic,S. et al.(2012). Concentration of Nitric Oxid in Saliva of Patient with Rheumatoid Arthritis .IJCRIMPH.,4(7):365-373 .
– Vanuffelen, B. E.; Van Derzec, J.and Dekoster, B. M. (2001)."Beta thalassaemia " Biochem J.;pp. 330.719.
- Hinton, P. R. Statistics explained. 2nd Edition by Routledge. printed in the USA and
Canada. 2004; 85: 125.
- Martins, D.B., Mazzanti, C.M., França, R.T., Pagnoncelli, M., Costa, M. M., De Souza, E. M., Gonçalves, J., Spanevello, R., Schmatz, R., Da Costa, P.(2012). 17-β estradiol in the acetylcholinesterase activity and lipid peroxidation in the brain and blood of ovariectomized adult and mi ddle-aged rats. Life Sciences; 90: 351–359.
- Xi, H. J., Wu, R. P., Liu, J. J., Zhang, L. J., Li, Z. S.Role of acetyl cholinesterase in lung cancer. Thorac Cancer, 2015; 6(4): 390-398.
- Fedorova, T., Knudsen, C. S. , Mouridsen, K.,Nexo, E., Borghammer,,P.(2015).Salivary acetylcholinesterase activity is increased in Parkinson’s disease: APotential marker of parasympathetic dysfunction. Parkinson’s Disease Volume 2015 , Article ID 156479, 7 pages.
- Pohanka, M. (2012). Alpha7 nicotinic acetylcholine receptor is a target in pharmacology and toxicology. Internat.J.Molecular.Scie. 13 (12):2219–2238.
- Nelson,DL.and cox,M.2008."Lehninger Principles of Biochemistry"5thed 1 New York,USA.pp:209-210.
- Ayala, A., Muñoz, M. F, Argüelles, S.(2014). Lipid peroxidation: Production,metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014: 360438.
- Das N, Chowdhury TD, Chattopadhyay A, Datta.(2004). Attenuation of oxidation stress - induced changes in thalassemic erythrocytes by Vitamin E. Polish Journal of Pharmacology,56: 85-96.
- Elalfy MS,Adly AA,Attia AA,Ibrahim FA,Mohammed AS ,S ayed AM.(2013):Effect of Antioxidant Therapy on Hepatic Fibrosis and Liver Iron Concentrations in β-Thalassemia Major Patients .Hemoglobin.Apr 9.
- Hasan F Al-Azzawi and Noor A Salman.2017:Association of Vitamin profile with Oxidant/ Antioxidant Status in Iraqi B-Talassemia Major Chldren. Jof Pharmacol&Clin Res;4(4); 555643 .
- Navaneet Krishnan S, Shaji G, Ravalya P, Nazia Sk (2013); Management of thalassemia; Int. Res. J. Pharm., 4:10; 1-3
- Elham A bed Mahdi .(2014).Relationship Between Oxidatve Stress and Antioxidant Stataus in Beta Thalassemia major patients .Acta Chimica and Pharmaceutica Indica:4(3),137-145 .
- Diez, E .E.; Miguel, V.; Mennerich, D.; Kietzamann,R. ;Sánchezperez, P.; Cadenas, S. and Lamas, S. (2015). Antioxidant responses and cellular adjustments to oxidative stress. Redox Biology, ELSIVER, 6: 183-197.
- Muaid SAS and Zaidan TF(2015): Oro-facial manifestation, oxidative stress marker and antioxidant in serum and saliva of patients with Beta thalassemia major .J Bagh Coll Dentistry;27(2):93-97 .
- Muanprasat C,Wongborisuth C, Pathomthongtaweechai V,Satitsri S,Hongeng S(2013):Protection against oxidative stress in beta thalassemia/hemoglobin E erythrocytes by inhibitors of glutathione efflux transporters . Acadimic J PLAS One .8(1).e55685 .
- Mamta Choudhary, et al. (2017) . Correlation of oxidative stress with serum antioxidant enzymes level in thalassemia in a tertiary level hospital of western Rajasthan, International Journal of Biotechnology and Biochemistry ;13(2),155-165
- Faruk , et al.(2018). Respiratory Burst Enzymes, Pro-Oxidants and Antioxidants Status in Bangladeshi Population with β-Thalassemia Major Uddin Shekhar , IP: 185.185.172.13
- Qaiser, S., Hoque, M. Z., Iqbal, M. and Mudin, D. K. D.( 2015). Evaluation of Antioxidant Status in Beta -Thalassemia Major Patients in Sabah, Malaysian Borneo. Biores Comm. 1(1), 45-47.
- N. Boudrahem-Addour, M. Izem-Meziane, K. Bouguerra et al.(2015). “Oxidative status and plasma lipid profile in β-thalassemia patients,” Hemoglobin, vol. 39, no. 1, pp. 36–41.
- Sandra Stella Lazarte María Eugenia Mónaco Cecilia Laura Jimenez, Miryam Emilse Ledesma Achem, Magdalena María Terán, and Blanca Alicia Issé.(2015). Erythrocyte Catalase Activity in More Frequent Microcytic Hypochromic Anemia: Beta-Thalassemia Trait and Iron Deficiency Anemia
- Satoshi Kono, Hiroaki Miyajima,(2015). Association of haptoglobin phenotypes with ceruloplasmin ferroxidase activity in β-thalassemia major. Rosenberg's Molecular and Genetic Basis of Neurological and Psychiatric Disease (Fifth Edition), P(1401-1424).
- Awadallah SM, et al, Association of haptoglobin phenotypes with ceruloplasmin ferroxidase activity in thalassemia majorClinChem.Acta,doil;10,1016/j.cca2011.02003.
- Jean P.DzoyemVictorKueteJacobus N.Eloff . (2014).Toxicological Survey of African Medicinal Plants., Pages 659-715 .
- Dugdale ,D.C. (2014) . ALP-Blood Test :Med Line Plus Medical Encyclopedia". Med Line Plus ,2014.
- Khaled M. Salama1, Ola M. Ibrahim2, Ahmed M. Kaddah1, Samia Boseila2*, Leila Abu Ismail2, May M. Abdel Hamid2 .(2015) . Liver Enzymes in Children with beta-Thalassemia Major: Correlation with Iron Overload and Viral Hepatitis.J of Medical Sciences,15;(2):287-292.
- Elalfy,M.S.;Esmat,G.;Matter,R.;Abdelaziz,H.and Massoud, W.H. (2013). "Liver fibrosis in young Egyptiaun Beta-thalassaemia major patients relation to hepatitis C virus and compliance with chelation". Annals of Hepatology ;12(1).p:45-50.
- Shams S, Ashtiani MTH, Monajemzadeh M, Koochakzadeh L, Irani H, Jafari F, et al.(2010) Evaluation of serum insulin, glucose, lipid profile, and liver function in β-thalassemia major patients and their correlation with iron overload. Labmedicine. 41(8): 486-9.
- Asmaa Nafady, Sanaa Shaker Ali, Hosny Mohammed Ahmed El Masry,Khaled Abdel Baseer, Heba M. Qubaisy, Samar Gallab Mahmoud,Hanaa A. Nafady-Hego.(2017). Oxidative stress in pediatric patients with β thalassemia major,Egyptian J of Hematology;42(3);p 123-127.
- Aessopos A, Kati M, Farmakis D.(2007) Heart disease in thalassemia intermedia: a review of the underlying pathophysiology. Haematologica ;92:658-65.
- Raafat N, Safy UE, Khater N, Hassan T, Hassan B, Siam A, et al. (2014).Assessment of Cognitive Function in Children With Beta-Thalassemia Major: A Cross Sectional Study. J Child Neurol,1−6.
- Nihayet Bayraktar, Mehmet Ali Erkurt, ‹smet Aydo.du, Yalc›n Baflaran.(2008).The levels of nitric oxide in beta-thalassemia minorTurk J Hematol ; 25: 187-9 .
- Rasheed, Z.; Ahmad, R.; Rasheed, N.; Ali, R.(2007) Reactive oxygen species damaged human serum albumin in patients with hepatocellular carcinoma. J. Expt. Clin. Cancer Res, 26, 515–524.
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