The Effect of Adding Linalol to Formalin on the Effectiveness of Embalming of Gastrocnemius Muscle
Keywords:
Linalol, Formalin, Embalming, Gastronemius.Abstract
Formaldehyde has been a standardized embalming agent. However, it has a strong odor and potential adverse health effects. Linalool, a natural fragrance, has been shown to have an antibacterial activity. This study aimed to determine the effect of adding linalool to formaldehyde on the effectiveness of embalming rat gastrocnemius muscle. In this posttest only control group design, 25 Wistar rats were randomly divided into 5 groups. Forty eight hours postmortem, the first group injected with 10 ml formaldehyde was used as control, the remaining three groups were injected with 10 ml formaldehyde in combination with 2.5 ml, 5ml or 67.5 ml or 10 ml of linalool respectively. Forty eight hours post mortem, the histologic features of the gastrocnimeus muscle of rats was assessed. The data were tested for normality and homogeneity followed by Kruskal Wallis test. Mean number of post mortem autolytic cells of gastrocnimeus muscles for the four groups were 1.88±3.67, 10.58±11.70, 15.00±12.65, 16.31±27.31, 4.49±2.93 respectively. There was no significant difference (p>0.05). Adding linalool to formaldehyde has no effect on the effectiveness of embalming of rat gastrocnimeus muscle.
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