Comparative Study on Callus Initiation and Regeneration Frequency of Two Salt Tolerant Rice (Oriza sativa)

Abstract

The objectives of this study were to find out the in vitro callus initiation and regeneration potentiality of two salt tolerant rice cultivars, viz., BRRI dhan47, BRRI dhan53. Mature seeds were used as explants. MS media supplemented with different concentrations of 2,4-D (1.0, 2.0, 3.0, 4.0 mg/l) were used for callus induction. The highest calli frequency was 85 % for BRRI dhan47 on MS media containing 3 mg/l 2,4-D, while other variety BRRI dhan53 showed maximum frequency of 75 % callus induction on MS media containing 3 mg/l 2,4-D. For complete plant regeneration the calli of two cultivars were plated on MS media containing different concentrations of kinetin, NAA (1-Naphthalene acetic acid) and BAP (6-benzyl aminopurine). The best regeneration frequency of BRRI dhan47 was 71.42 % on MS media containing 2 mg/l kinetin, 2 mg/l BAP and 1 mg/l NAA and it was 87.50 % for BRRI dhan53 on MS media containing 2 mg/l kinetin, 1 mg/l BAP and 1 mg/l NAA.