Association of cGH EcoRV Gene with Production in Tolaki Chicken

Authors

  • Muhammad Amrullah Pagala Halu Oleo University
  • Andi Murlina Tasse Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, Jl Agatis Kampus IPB, Darmaga, Bogor 16680
  • Niken Ulupi Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, Jl Agatis Kampus IPB, Darmaga, Bogor 16680

Keywords:

biotecnology

Abstract

cGH (Chicken Growth Hormone) gene plays a crucial role in production responses of chicken. The objective of the research was to investigate the association of cGH gene with production in Tolaki chicken. Tolaki chicken is native chickens from Southeast Sulawesi Province of Indonesian. cGH gene was genotyped in 58 Tolaki chicken with PCR-RFLP. PCR was used to amplify genomic DNA for GH gene (399 bp). The amplicon was cutted by EcoRV and produced three genotypes: AA, AG, and GG and two alleles: A and G allele. The study showed the association of GH gene polymorphism with Production traits. GG genotype have better production (daily weight gain and feed conversion) than AG genotype in Tolaki chicken, providing evidence that GH gene might be an important candidate gene for production traits.

Author Biography

Muhammad Amrullah Pagala, Halu Oleo University

Departmen of Animal Production,

References

Alexander DJ.. Newcastle Disease. British Poult Sci. Vol.42,pp.5

Bingxue, Y., D.Xuemei, F.Jing, H. Xiaoxiang., W Changxin & L Ning. Single nucleotide polymorphism analysis in chicken growth hormone gene and its associations with growth and carcass traits.Chinese Science Bulletin Vol. 48.pp. 1561-1564.2003.

Fulton, J., H. R. Juul-Madsen, C. M. Ashwell, A. A. McCarron, J. A. Arthur, N. P. O

Maskur, C. Arman, C. Sumantri, E. Gurnadi, & Muladno. A novel single nucleotida polymorphism in exon 4 of insulin-like growth factor-1 associated with production traits in Bali cattle. : Med. Pet. Vol.35, pp.96-101,2012.

Matjik, A. A. & M. Sumertajaya.

Otim, M. O., H. Christensen, P. H. J

Pagala, M. A., Muladno, C.Sumantri & S. Murtini. . Association of Mx Gene Genotype with Antiviral and Production Traits in Tolaki Chicken. J. Poult Sci. Vol.12,pp.735-739. 2013.

Pagala, M. A. & L. O. Nafiu. The molecular identified of antiviral traits of Tolaki chicken by detection Mx gene as a genetic marker. J.Agriplus. Vol.23.pp.139-144. 2012.

Sambrook, J., F. Fritsch, & T. Maniatis.. Molecular Cloning. A Laboratory Manual 2nd ed. Cold Spring Harbor Laboratory Press. 1989.

Sulandari, S., M. S. A. Zein, D. Astuti, & T. Sartika. Genetic polymorphisms of the chicken antiviral Mx gene in a variety of Indonesian indigenous chicken breeds. J. Vet, Vol.10,pp.50-56. 2009.

Renate, A.

Sumantri, C., R. Diyono, A. Farajallah, A. Anggraeni, & E. Andreas.

Suprijatna, E. & D. Natawihardja..

Ulupi, N, Muladno, C.Sumantri, IWT Wibawan,.

Zhou H,. AD Mitchell, JP McMurtry, CM Ashwell, and S. J. Lamont.. Insulin-Like Growth Factor-I Gene Polymorphism Associations with Growth,Body Composition, Skeleton Integrity, and Metabolic Traits in Chickens. Poultry Science,Vol. 84,pp.212

Downloads

Additional Files

Published

2015-12-13

How to Cite

Pagala, M. A., Tasse, A. M., & Ulupi, N. (2015). Association of cGH EcoRV Gene with Production in Tolaki Chicken. International Journal of Sciences: Basic and Applied Research (IJSBAR), 24(7), 88–95. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/4987

Issue

Vol. 24 No. 7 (2015)

Section

Articles

License

Authors who submit papers with this journal agree to the following terms.