Susceptibility Pattern Of Pseudomonas Aeruginosa Against Various Antibiotics
Keywords:
P. Aeruginosa, Antibiotics, Antibiotic Resistance, Nosocomial Infections, DockingAbstract
P. aeruginosa, an increasingly prevalent opportunistic human pathogen, is the most common gram-negative bacterium responsible for the nosocomial and community acquired infections. The excessive use of antibiotics has not only led to treat the infections but also the emergence of antibiotic resistance. Multidrug resistant P. aeruginosa development is currently one of the greatest challenges. Total 100 clinical isolates of P. aeruginosa from inpatient and outpatient were studied. Uropathogenic P. aeruginosa infections were higher in females than males, ratio was found more among young and elderly debilitated patients. 99% of the clinical isolates were resistant to six commonly used antibiotics with the most resistant pattern being Ampicillin (100%), Amoxycillin (99%), Co-trimoxazole (99%), Tetracycline (99%), Cefazoline (99%) and Cefuroxime (100%). The invitro sensitivity pattern of 100 isolates of P. aeruginosa showed Imipenem (97%), Amikacin (79%), Tobramycin (70%), Ceftazidime (62%), Ciprofloxacin (73%), Cefoperazone (60%), Piperacillin (65%), Gentamycin (34%) and Cefotaxime (14%) sensitivity. ESBLs producing strains (33%) were also less in number but were much more resistant to ?-lactam and other antibiotics. Docking of both effective drugs against bacteria (Amikacin and Imipenem) with least resistance 21% and 3%, respectively, analyzed how these drugs interact with envelope protein to stop its growth.
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