Effect of Alcohol and Nicotine on Fertility of Male Albino Mice

Authors

  • Md Rezwan Hussain Department of Biotechnology Gauhati University
  • G. U. Ahmed
  • Rita Mahanta

Keywords:

Male infertility, Alcohol and Nicotine, Rbm

Abstract

Abuse of recreational drugs such as alcohol and nicotine may be genotoxic and may have detrimental effect on the male fertility which was aimed to be investigated through this present study using mice model systems. A total of 60 mice were divided into three groups, viz.; control group (n=20), and two groups of alcohol treated (n=20) and nicotine treated mice groups (n=20), administered in recommended dose amount for 90 days. Representative numbers of mice were sacrificed on 10th, 30th and 90th day from each group and the testes were collected. DNA and RNA was extracted from the collected tissue following standard protocol; followed by RAPD-PCR and Real time PCR based analysis of extracted DNA and cDNA, prepared from isolated RNA, for Rbmy1 gene expression analysis, respectively. ?-actin was used as internal normalization control for Real time PCR analysis. Remarkable changes were observed in the RAPD profiles, based on difference in band pattern profile as observed in alcohol treated and nicotine induced cases compared to controls; thereby clearly indicating the effect of recreational drug on the genome of the host in comparison to untreated controls. The differential expression profile of Rbmy1 mRNA in the nicotine model, showed significant down regulation compared to control (p=0.045), and non-significant when compared to alcoholic model cohorts (p=0.307).The observation also showed differences of Rbmy1 mRNA expression in alcoholic cases compared to controls (p=0.782).

The present study indicates that consumption of recreation drugs such as nicotine may play an important role in disturbing the genome integrity and expression of key gene associated with male fertility, thereby disposing to male infertility.

Author Biography

Md Rezwan Hussain, Department of Biotechnology Gauhati University

PhD Research Scholar

References

A K Armitage,G H Hall and CF Morrison

A Riesenfeld,H Oliva

A Sano,T Funabashi,M Kawaguchi,K Shinohara,F Kimura

Andrey E.Ryabinin,Mark N Miller,Shane Durrant : Effects of Alcohol administration on object recognition learning in C57BL/6J mice;Pharmacology,Biochemistry and behaviour 71 (2002)307-312

B Peterlin,T Kunej,D Hristovsk,

C Lerman, R Tyndale, F Patterson, EP Wileyto, PG Shields,A Pinto, et al.

Carolyn M Fronzak,Edward D.Kim and Al B.Barqawi.

CH Ramlau-Hansen,AM Thulstrup,AS Aggerholm,MS Jensen,G Toft,JP Bonde

D Yildiz,Y.S Liu,N Ercal, D.W Amstrong

GP Donnelly, N McClure, MS Kennedy, SEM Lewis.,

H Skaletsky,T Kuroda Kawaguchi,P.J Minx,H.S Cordum,L Hillier et al

H.J Cooke and P.T.K Saunders

J Buffum.

J.G Williams,A.R Kubelik,K.J Livak,J.A Rafalski,S.V Tingey

J.M Hopkins,H.J Evans,

JF Pankow A consideration of the role of gas/particle partitioning in the deposition of nicotine and other tobacco smoke compounds in the respiratory tract. Chem ResToxicol; 14:1465-1481. 2001.

L Tiepolo and O Zuffardi

M Maneesh, S Dutta, A Chakrabarti, DM Vasudevan

M Mangubat,K Lfty,ML lee,L Pulido et al., Effect of nicotine on body composition in mice

M. Arabi

M.C. Lardone, D.A.Parodi,Valdevenito, R.Ebensperger et al.,

M.L. Hansen, A.M Thulstrup, J.P Bonde et al., Does last week

MM Matzuk, DJ lamb.

N.J Jones,M.A. Kadhim,P.L Hoskins,et al

N.Z.I El-Alfy, M.F.Mahmoud and A.M Abdullah

P.H Vogt,A Edelmann,S Kirsch,O Henegariu,P Hirschmann,et al.,

R Lavery, M.Glennnon, J. Houghton , A Nolan, D Egan, M. Maher

R Peakall and P.E Smouse GENALEX 6: genetic analysis in Excel.Population genetic software for teaching and research

R Reijo, T.Y Lee,P Salo,A Raaji, G.B.Laura,R Michael et al.,

R. Hussain,G.U Ahmed and R.Mahanta

R.M Sharpe ,

R Sharma , K Biedenharn , J Fedor, A Agarwal A. Lifestyle factors and reproductive health:taking control of your fertility. Reprod Biol Endocrinol 2013;11:664

S Purkayastha and R Mahanta

S Sen and A Sharma

S Sepaniak,T Forges,B Fontaine,H Gerard,B Foliguet,F Guillet May et al., Negative impact of cigarette smoking on male fertil- ity: from spermatozoa to the offspring. J Gynecol Obstet Biol Reprod;33(5):384

S Zhang, I Day, S Ye

X Meng, M Lindahl, Me Hyvonen, M Parvinen, DG de Rooij, MW Hess, A Raatikainen Ahokas, K Sainio et al.,

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Published

2015-08-15

How to Cite

Hussain, M. R., Ahmed, G. U., & Mahanta, R. (2015). Effect of Alcohol and Nicotine on Fertility of Male Albino Mice. International Journal of Sciences: Basic and Applied Research (IJSBAR), 24(1), 442–452. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/4493

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