Circulation of Dengue Serotypes in Local Population of District Lahore, Pakistan

Authors

  • Ammad ud din Institute of molecular biochemistry and biotechnology, The university of Lahore
  • imran riaz malik Department of Biotechnology, University of Sargodha.
  • Rabail Alam
  • Ghulam Mujtaba
  • Mehmood-ul-Hassan Qazi

Keywords:

Dengue, Envelope Encoding Gene, IgG, IgM, Serotypes Introduction.

Abstract

Infection with dengue virus (DENV) is considered as serious public health issues internationally as amounted to 2.5 billion people are at infection risk throughout the world. Dengue is now endemic in Pakistan. Till now, no licensed vaccine is available against dengue virus infection. The main purpose of this study was to find out differences in the levels of IgM and IgG on gender basis as well as distribution of dengue serotypes in the local population of district Lahore, Pakistan. Fifteen blood samples including 3 control sample were collected from dengue infected patients and statistical results showed significantly higher mean levels of IgM (1.12 + 0.09) and IgG (2.07 + 0.56) antibodies in patients as compared to control groups for IgM (0.34 + 0.05) and IgG (0.10 + 0.05) antibodies respectively. Statistical results on Gender base showed significantly higher mean levels of IgM (1.10 + 0.19) in males and of IgG (2.27 + 0.74) in females. The enveloped gene of 1.5 kb was successfully amplified through polymerase chain reaction and cloned in ?TZ57R/T. The cloned gene was then confirmed through restriction digestion.

Out of 15 dengue samples, only 3 dengue samples were successfully amplified using polymerase chain reaction which all belongs to serotype 2 In future, it may contribute in development of treatment to dengue infection with dengue virus type 2 which is more prevalent in district Lahore

Author Biographies

Ammad ud din, Institute of molecular biochemistry and biotechnology, The university of Lahore

MPhil student, IMBB

imran riaz malik, Department of Biotechnology, University of Sargodha.

Assistant professor, Department of Biotechnology

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Published

2015-02-22

How to Cite

din, A. ud, malik, imran riaz, Alam, R., Mujtaba, G., & Qazi, M.- ul-H. (2015). Circulation of Dengue Serotypes in Local Population of District Lahore, Pakistan. International Journal of Sciences: Basic and Applied Research (IJSBAR), 20(2), 49–58. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/3329

Issue

Vol. 20 No. 2 (2015)

Section

Articles

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