Survey Study of the Allergic Fungi in Kirkuk Area and Use Molecular Detection for Identification

Authors

  • Sanaa Huseein Mohamad IRAQIA UNIVERSITY
  • Karkaz M. Thalij
  • Khalil AL-Bander
  • Batol Imran Dheeb

Keywords:

Allergic fungi, Outdoor, Indoor fungi, PCR

Abstract

The study was carried out at the period from 1st February 2013 until 31st January 2014 in order to determine the relationship of the allergic fungi with the lower bronchial allergy patients and study the occurrence of fungal in indoor and outdoor building. Two hundred samples of sputum were collected from the respiratory tract infected patients and 20 samples sputum from healthy persons, which ranged in age from less than five to more than 51 years of both sexes the results showed that the rate of infection among examined cases were 134 (67%), by direct microscopy and culturing. The rate of infection in females was higher78 (58.2%) than males 56 (41.7%).The distribution of positive cases

References

Kowalski, W.; Bahnfleth, W.P. (1998). Airborne respiratory diseases and technologies for control of microbes. Heating Piping Air Conditioning,70 (6), 34

Ross, M.A.; Curtis, L.;Scheff, P.A.; Hryhorczuk. D .O. ; Ramakrishnan , V.;Wadden, R.A.;Persky, V.W.( 2000). Association of asthma symptoms and severity with indoor bioaerosols. Allergy,55: 705

Horner ,W.E.; Helbling ,A.; Salvaggio, J.E. and Lehrer, S.B. (1995) .Fungal allergens. Clin. Microbiol .Rev., 8: 161-79.

Anderson, M.J.; Gull ,K .and Denning, D.W. (1996). Molecular typing by random amplification of polymorphic DNA and M13 southern hybridization of related paired isolates of Aspergillus fumigatus. J. Clin . Microbiol, 34 (1): 87-93.

Flannigan, G.; Hammer, S. and Groumet,R.(1991).Aquick and inexpensive method for removing polysaccharides from plant genomic DNA. Biofeed back.13:52-54.

Wald, A.;.Leisenring, J. A. ;Van ,B. and Bowden, R. A. (1997). Epidemiology of Aspergillus infections in a large cohort of patients undergoing bone marrow transplantation. J. Infect. Dis. ,175

Latg

Waring, P.; Eichner, R.D.; M

Kamei, K. and Watanabe, A. (2005). Aspergillus mycotoxins and their effect the host. Med .Mycol. 43 Suppl.,1: S95-99.

Orciuolo,E.;Stanzani,M.;Canestraro,M.;Galimberti,S.;Carulli,G.;Lewis,R.;Petrini,M. and Komanduri, K.V.(2007).Effects of Aspergillus fumigatus gliotoxin and methyl prednisolone on human neutrophils: implications for the pathogenesis of invasive aspergillosis. J. Leuko . Biol.,82:839-848.

Malicev, M.; Chowdhury, H. H.; Macek, P. and Sepcic, K.(2007) . Effect of ostreolysin, an Asp-hemolysin isoform, on human chondrocytes and osteoblasts, and possible role of Asp-hemolysin in pathogenesis. J.Medical Mycology, 45: 123_130 .

Monod, M.; Fatih, A.; Jaton-Ogay, K.; Paris, S, and Latg

Ellis, M. (1999).Therapy of Aspergillus fumigatus-related diseases. Contrib Microbiol ,2:105-129.

Latg

Midgley,G.; Clayton, Y.M .and Hay, P.R. J. (1997). Diagnosis in color medical mycology. 1st Ed., Mosby-Wolf.

Emmons, C. W.; Binford, C. H.; Utz, J. P .and Kownchung, K. J. (1977).Medical Mycology, 3rd Ed. Lea and Febiger, Philadelphia.

Jawetz, E.; Melnick, J. and Adelberg, E.A. (1991). Medical Microbiology. 20th Ed., Middle East edition. P: 532-546.

Forbes, B.; Sahm, D . and Weissfeld, A. (1998).Bailey and Scott

Samson, R. A.( 1994). Current systematic of the genus Aspergillus, p. 261

Larone, D.H. (1995) . Medically important fungi: Aguide Fitzpatrick

Kirk, P.M . ; Cannon , P.F . ; David, J. C. and Stalpers, J. A. (2008) . Ainsworth and Biby

Toshio, H. F.(2002).The developmentally regulated ab1 gene of Asperegillus fumigatus ils role in modulation of conidial morphology and virulence. J.Bacteriol ,180:3031-3038.

Yamamoto, N.;Shendell, D.G. and Peccia, J. (2011).Assessing allergenic fungi in house dust by floor wipe sampling and quantitive PCR.Indoor Air,21:521-530.

Sambrook, J.; Friteg, E. F. and Maniatis, T. (1989). Molecular cloning : a laboratory . New York, USA.

Larone, D.H. (1995) . Medically important fungi: Aguide Fitzpatrick

Soubani, V.;Hartl, A.; Brock, M.;Hubner, K.; Rohde, M.;Heinekamp, T. and Brakhage, A. A.(2011). Characterization of the lactase-encoding geneabr2 of the dihydroxynaphthalene-like melanin gene cluster of Aspergillus fumigatus. Arch.Microbiol.,186: 345-355.

Maiz, L.; Cuevas,M.;Lamas.A.;Sousa,A.;Quirce,S. and Suarez, L. (2008).Aspergillus fumigatus and Candida albicans in cystic fibrosis :Clinical significance and specific immune response involvingserum immunoglobulins G,A, and M. Arch Bronconeumol., 44(3):146-51.

Arruda,L.K.;Mann,B.J.and Chapman,M.D.(1992a).Selective expression of amajorallergen and cytotoxin, AspfI, in Aspergillus fumigatus. Implications fortheimmunopathogenesisofAspergillus-relateddiseases.J.Immunol,149:3354-3359.

Al- Ameed,A.I.M.(2008) . Isolation of Aspergillus fumigatus from Human Being and the study of immune response on rabbits immunized by their antigens .MS. Thesis. College of Veterinary Medicine

AL-Malikey, H.S. (2009) . Effect of alcoholic extract of callistemon viminalis (Sol.exGaertn) G.D on fungi cause aspergillosis in albino mice. College of science - Baghdad University, Iraq.

Deeb, O. B. (2013). Study on the pathogenicity, cytotoxicity and virulence factors of some clinical of Aspergillus fumigatusisolates and the use of RAPD markers to distinguish them. Ph. D. ThesisCollege of Science. Tikrit. University, Iraq.

Komase,R. ; Nakamura, Y. and Watari, T. (2007) .Phylogenic analysis of 8 lower respiratory tract infection species using chitin synthase 1 gene sequences. Mycoses , 40:411-414

De Rosa, G.S. and Guarro, J. (2009) . effects of lower respiratory tract infection on physiological paramters using Schimelcultures / Universitat Rovira I Virgili : 134- 638.

pasanen, Pina-Vaz, C. ; Salgueiro, L. ; Goncalves, M.J. Health Laboratory Service Spa Pools Working Group (2000) . Hygiene for Spa pools, London . Blackmore Press, ISBN 0901144371.

Shelton,B.G.; Kirland,K.H.; Flanders,W.D. and Morris ,G.K .(2002) .Profiles of airborne fungi in buildings and outdoor environments in the United States.Applied and Environmental Microbiology,68(4):1743-1753.

Condia,R.;Fox,R.W.; Jockey,R.F.;de Marco, P. and Bagg, A. (2008). Typical levels of airborne fungal spores in houses without obvious moisture problems during a rainy season in Florida,USA.Journal of Investigational Allergology and Clinical Immunology ,18(3),156-161.

Meklin, T.;Husman, T.;Vepsalainen, A.;Vahteristo, M.;KoivistoJ;Halla

Rand,T.(1999).An assessment of mould contamination problems in Atlantic Canada schools:Moldburdens,amplifying sites and benefits of proactive school inspection policies ,In:Johanning, E.(ed.)Bioaerosols,Fungi and Mycotoxins:Health Effects,Assessment,Prevention and Control,Albany,Eastern New York Occupational and Environmental Health Center,581-592.

Reponen,T.;Nevalainen,A.;Jantunen,M.;Pellikka,M.and Kalliokoski,P.P.(1992).Normal range criteria for indoor air bacterial and fungal spores in a subarctic climate. Indoor Air,2:26-31.

Hyvarinen, A.; Reponen, T.;Husman,T.;Ruuskanen, J. and Nevalainen, A.(1993). Characterizing mold problem buildings concentrations and flora of viable fungi, Indoor Air,3:337-343.

Dotterud,L.K.;Vorland,L.H.and Falk,S.(1995).Viable fungi in indoor air in homes and schools in the SOr-Varanger community during winer,Pediatr.Allergy Immunol.,6:181-186.

Awosika, S.A.; Olajubu,F.A. and Amusa, N.A. (2012). Microbiological assessment of indoor air of a teaching hospital in Nigeria. Asian Pacific Journal of Tropical Biomedicine, 465-468.

Ekhaise,F.O.;Isitor,F.F.;Idehen,O.andEmogbene,O.A. (2010). Airborne microflora in the atmosphere of an hospital environment of University of Benin Teaching Hospital (UBTH),Benin City,Nigeria.World J.Agric.Sci.;6(2):166-170.

Hsu, N.Y.; Lee, C.C.; Wang, J.Y.; Li, Y. C.; Chang, HW; ChenCY; Bornehag, C. G.; Wu,P.C.; Sundell, J. and Su, H.J. (2012).Predicted risk of childhood allergy ,asthma , and reported symptoms using measured phthalate exposure in dust and urine .Indoor Air,22:186-199.

Rojas, T.I. and Aira, M.J. (2012). Fungal biodiversity in indoor environments in Havana, Cuba .Aerobiologia,28: 367-374.

Aira,M.J.;Rojas,T.I. and Jato,V.(2002).Fungi associated with three houses in Havana (Cuba).Grana ,41:114-118.

Labarrere,N.;Gomez,A.;Avila,I.;Guevara,M.E.and Fernandez,B. (2003) . Riesgosbiologicos en ambientesconfinados.RevistaCubana de Saludy Trabajo,4(1-2),4-7.

Borrego,S.;Guianmet,P.Gomez de Saravia,S.;Batistini,P.; Garcifa,M.;Lavin,P., et al.,(2010).The quality of air at archives and the biodeterioration of photographs.International Biodeterioration and Biodegradation,64:139-145.

Medrela-Kuder,E.(2003).Seasonal variation in the occurrence of culturable airborne fungi in outdoor and indoor air in Cracow. International Bioderioration and Biodegradation,52(4):203-204.

Hedayati,M.T.;Mayahili,S.;Aghili,R. and Goharimoghadam, K. (2005). Airborne fungi in indoor and outdoor of asthmatic patients home,living in city of Sari.Iran Journal of allergy Asthma and Immunology,4(4):189-191.

Pasanen,A.L.;Pasanen,P.P.;Jantunen,M.J. and Kalliokoski,P. P. (1991). Significance of air humidity and air velocity for relationship between measured moisture conditions and fungal concentrations in water-damaged building materials. Indoor Air ,10(2),111-120.

De Hoog, G.S.(1996).Risk assessment of fungi in humans and animals.Diagnosis,therapy and prsphyl of fungal diseases .Mycoses,39(11 / 12 ),407-417.

De Lucca,A.J.(2007).Harmful fungi in both agriculture and medicine . RevistaIberoamericana de Micologia,24:3-13.

Cho ,S.J.; Ramachandran, G.; Banerjee, S.; Ryan ,A.D. and Adgate, J.L. (2008). Seasonal variability of culture able fungal genera in the house dust of inner-city residences, J. Occupy Environ Hyg.,5: 780

Magnuson, J. and Lasure, L. (2004). Organic acid production by filamentous fungi. In: Tkacz J, Lange L (eds). Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine. New York: Kluwer Academic and Plenum Publishers, 307-340.

Vengayil, S.; Panda, A.; Satpathy, G.; Nayak, N.; Ghose, S.; Patanaik, D. and Khokhar, S. (2009). Polymerase Chain Reaction

Kaliamurthy, J. ; Nelson, J. C. ; Kalavathy, C. M. ; Parmar, P. and Thomas, P. A. (2008). Efficacy of Polymerase Chain Reaction (PCR) as a Rapid Diagnostic Aid for Keratomycosis. Cornea.,3:198-199.

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Published

2015-01-09

How to Cite

Mohamad, S. H., Thalij, K. M., AL-Bander, K., & Dheeb, B. I. (2015). Survey Study of the Allergic Fungi in Kirkuk Area and Use Molecular Detection for Identification. International Journal of Sciences: Basic and Applied Research (IJSBAR), 19(1), 383–397. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/3241

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