Growth Response of Four Nile Tilapia Strains Fed on Diet Containing a Recombinant Teleostean Growth Hormone

Authors

  • Muhammad Az
  • Muhammad Zairin Jr
  • . Alimuddin
  • Odang Carman

Keywords:

Nile tilapia, recombinant growth hormone, RNA/DNA ratio, strains

Abstract

Various Nile tilapia strains arecultured in Indonesia. This study was conducted to examine the growth response of four Nile tilapia strains, namely SULTANA (superior selected tilapia strain from Selabintana), NIRWANA (tilapia strain from Wanayasa), SRIKANDI (salinity resistant improvement from Sukamandi), and red tilapia fed on diet containing recombinant Epinephelus lanceolatus growth hormone (rElGH). In the first study, red tilapia were reared in 200-L glass aquaria and fed on artificial diet with different doses of crude rElGH protein, namely 0 (control), 0.03, 0.30, and 3.00 mg/kg diet. The results showed that growth, specific growth rate (SGR), and feed conversion ratio (FCR) were notsignificantly differentamongtreatments (p>0.05), but significantly different when comparedto the control (p<0.05).The second study was performed in the hapa (2x1x1m3) settled in a concrete pond (20x10x1.5 m3). By using 3 mg rElGH/kg diet, the results ofthesecond study showed that the highest (p<0.05) SGR was obtained in SULTANA (3.73%), followed by NIRWANA (3.41%). SRIKANDI (3.36 %),and red tilapia (3.14%) strain. Lowest FCR (p<0.05) was found in SULTANA (0.84), followed by NIRWANA (0.99), SRIKANDI (1.02), and red tilapia (1.22) strains. Survival of the four strains were similar, ranging from 84.67 to 90.00% (p>0.05). The highest (p<0.05) liver and muscle glycogen, protein and fat retention, and RNA/DNA ratio were found in SULTANA, followed by NIRWANA,

References

S. Sekine, T. Mizukami, T. Nishi, Y. Kuwana, A. Saito, M. Sato, S. Itoh, H. Kawauchi. 1985. Cloning and expression of cDNA for salmon growth hormone in Escherichia coli. Proc. Natl. Acad. Sci. USA. 82:4306-4310.

B. Promdonkoy, S. Warit, S. Panyim. 2004. Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli. Biotechnol Lett. 26: 649-653.

H.P. S

Alimuddin, I. Lesmana, A.O. Sudrajat, O. Carman, I. Faizal. 2010. Production and bioactivity potential of three recombinant growth hormones of farmed fish. Indones Aquac J. 5:11-16.

J. Acosta, R. Morales, A. Morales, M. Alonso, M.P. Estrada. 2007. Pichia pastoris expressing recombinant tilapia growth hormone accelerates the growth of tilapia. Biotechnol Lett. 29: 1671-1676.

S. Moriyama and H. Kawauchi. 2001. Growth regulation by growth hormone and insulin-like growth factor-I in teleosts. Otsuchi Mar Sci. 26:23-27

D. Santiesteban, L. Mart

Y. Li, J. Bai, Q. Jian, X. Ye, H. Lao, X. Li , J. Luo, X. Liang. 2003. Expression of common carp growth hormone in the yeast Pichia pastoris and growth stimulation of juvenile tilapia (Oreochromis niloticus). Aquaculture. 216 : 329-341.

D. Hardiantho, Alimuddin, A.E. Prasetyo, D.H. Yanti, K. Sumantadinata. Aplikasi Rekombinan Growth Hormon (rGH) Ikan Mas pada Ikan Nila Melalui Pakan Buatan. Makalah yang disampaikan dalam Pertemuan Broodstock Center Nila & Temu Koordinasi Perekayasa Kementerian Kelautan dan Perikanan, Sukabumi Indonesia, 15-17 November 2011. (in Indonesian).

S. Moriyama, H. Yamamoto, S. Sugimoto, T. Abe, T. Hirano, H. Kawauchi. 1993. Oral administration of recombinant salmon growth hormone to rainbow trout, Oncorhynchus mykiss. Aquaculture. 112: 99

H.J. Tsai, M.H. Hsih, J.C. Kuo. 1997.Escherichia coli produced fish growth hormone as a feed additive to enhance the growth of juvenile black seabream (Acanthopagrusschlegeli). J.Appl.Ichthyol. 13: 78-82.

B. Funkenstein, A. Dyman, Z. Lapidot, E.G. de Jesus-Ayson, A. Gertler, F.C Ayson. 2005. Expression and purification of a biologically active recombinant rabbitfish, Siganus guttatus growth hormone. Aquaculture. 250 : 504-515.

S. Liu, X. Zhang, X. Zang, B. Liu, K.K.I.U. Arunakumara, D. Xu, X. Zhang, 2008. Growth, feed efficiency, body muscle composition, and histology of flounder (Paralichthys olivaceus) fed GH transgenic Synechocystis. Aquaculture 277: 78

B. Handoyo .2012.ResponsBenihIkanSidatterhadapHormonPertumbuhanRekombinanIkanKerapu Kertang Melalui Perendaman dan Oral. [Thesis]. Institut Pertanian Bogor.Bogor (ID) (in Indonesian with English abstract).

Irmawati, Alimuddin, M. Zairin Jr, M.A. Suprayudi, A.T. Wahyudi. 2012. Peningkatanlajupertumbuhanbenihikangurame (OsphronemusgoramyLac.) yang direndamdalam media yang mengandunghormonpertumbuhanikan mas. J IktiolIndones.12(1).(in Indonesian with English abstract).

M. Safir. 2012. Respons Benih Ikan Gurami (Osphronemus goramy) yang diberi Hormon Pertumbuhan Rekombinan Melalui Oral pada Dosis Berbeda. [tesis]. Institut Pertanian Bogor. Bogor (ID) . (in Indonesian with English abstract).

S. Subaidah, O. Carman, K. Sumantadinata, Sukenda, Alimuddin, 2012. Respons pertumbuhan dan ekspresi gen udang vaname Litopenaeus vannamei setelah direndam dalam larutan hormon pertumbuhan rekombinan ikan kerapu kertang. J. Ris. Akuakultur. 7 (3): 337

Irmawati 2013. Respons Fisiologis, Biokimia, dan Molekuler Ikan Gurame yang diberi Hormon Pertumbuhan Rekombinan. [Disertasi]. Institut Pertanian Bogor. Bogor (ID). (in Indonesian with English abstract).

Y. Hertz, A. Tchelet, Z. Madar , A. Gertler. 1991. Absorption of bioactive human growth hormone after oral administration in the common carp and its enhancement by deoxycholate. J Comp Physiol, 161:159-163.

AOAC. 2000. In: K. Helrich. (Ed). Official Methods of Analysis. 15th ed. Association of Official Analytical Chemist, Arlington, VA. USA.

J.T. Silverstein, W.R. Wolters, M. Shimizu, W.W. Dickhoff . 2000. Bovine growth hormone treatment of channel catfish: strain and temperature effects on growth, plasm IGF-I levels, feed intake efficiency and body composition. Aquaculture. 190:77

G. Walsh. Biopharmaceuticals, Biochemistry and Biotechnology. Second Edition. England, John Wiley & Sons Ltd. 2003.p 551.

R.S. Rasmussen, B. R

A. Vijayakumar, R. Novosyadly, Y. Wu, S. Yakar, D. LeRoith. 2010. Biological effects of growth hormone on carbohydrate and lipid metabolism. Growth Horm IGF Res. 20:1-7.

H. Gl

S. Subaidah. 2013. Respons Pertumbuhan dan Imunitas Udang Vaname Litopenaeus vannamei terhadap Pemberian Hormon Pertumbuhan Rekombinan Ikan Kerapu Kertang [disertasi). Institut Pertanian Bogor. Bogor (ID). (in Indonesian with English abstract).

Downloads

Published

2014-06-11

How to Cite

Az, M., Jr, M. Z., Alimuddin, ., & Carman, O. (2014). Growth Response of Four Nile Tilapia Strains Fed on Diet Containing a Recombinant Teleostean Growth Hormone. International Journal of Sciences: Basic and Applied Research (IJSBAR), 16(1), 397–406. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/2365

Issue

Section

Articles