Determination of the Effect of the Environment on the Genetic Polymorphism In the Genus of Tamarix Using the Molecular Marker ( Simple Sequence Repeats "PCR-SSR" (In Arid Areas of the Khenchela Region (Eastern of Algeria)

Authors

  • Khabtane Abdelhamid Abbes Laghrour University
  • Rahmoune Chaabane Constantine University
  • Ben Naceur M'barek inrat of Tunis
  • Rabaaoui Sihem inrat of Tunis
  • Aouidane Elaiche abbes Laghrour University
  • Kadi Kezan Abbes Laghrour University

Keywords:

Tamarix, arid regions, genetic polymorphism, PCR-SSR, Khenchela, Algeria

Abstract

Tamarix is used for a long time as a treatment plant in traditional and modern medicine because of its healing powers. In addition to these medicinal characteristics Tamarix has a great adaptation to the extreme environmental conditions, especially in arid and semi arid regions, where it represents a promising source for rehabilitation of degraded ecosystems and fight against desertification. However, to date, only limited information about its genetic structure. To attempt to characterize the genetic diversity of the species of this genus is therefore very useful for their classification, their conservation and improvement.

In this context, we started in the assessment of the level of genetic diversity characterizing Tamarix pushing currently in Algeria and in which they are originate. we used the PCR-SSR of 23 genotypes selected from three very different sites (en perspective of climate, edaphic and orographic conditions), through the arid regions of Khenchela in eastern of Algeria. The results are obtained by the highlighted amplification of 14 primers specific for Tamarix. Analysis of the results using the software TreeCon for Windows, at a threshold of dissimilarity of 50% we have obtained nine distinct groups, in general, the results showed a high level of genetic divergence within and between species among genotypes, with relevant remarks in four accessions, (II 2, II 10, III 3 and III 9), of the ninth

Author Biographies

Khabtane Abdelhamid, Abbes Laghrour University

department of biology

Rahmoune Chaabane, Constantine University

department of biology

Ben Naceur M'barek, inrat of Tunis

genetic laboratory

Rabaaoui Sihem, inrat of Tunis

genetic laboratory

References

Baum, B. R. Introduced and naturalized tamarisks in the United States and Canada (Tamaricaceae). Baileya 15:19-25. 1967.

Quezel P. et Santa S. Nouvelle flore de l'Alg

Kadi K. et al. In vitro antibacterial activity and phytochemical analysis of white henbane treated by phytohormones, Pakistan journal of biological Sciences 16 (19) 984-990. 2013.

Khabtane A. et Rahmoune C., Effet du biotope sur la diversit

Khabtane A. et Rahmoune C., 2010. contribution

M

Saghi-maroof M. A., Biyashev R. M., Yang G. P., Zhang Q. and Allard R. W., Extraordinarily polymorphic microsatellite R. W. DNA in barley: Species diversity chromosomal locations, and population dynamics. Proc Nalt Acad Sci, 91: 5466-5470. 1994.

Serena T. et al, development of a novel set of EST-SSR markers and cross-species amplification in Tamarix africana (Tamaricaceae), Primer Notes & Protocols in the Plant Sciences, American Journal of Botany 97 (6): e45. 2010.

Gaskin, J. F., Adam S. Birken and David J. Cooper. Levels of novel hybridization in the saltcedar invasion compared over seven decades. Biological Invasions. 14 (3): 693-699. 2012.

Gaskin, J.F. and B.A. Schaal. Molecular phylogenetic investigation of invasive Tamarix in the U.S.A. Syst. Bot. 28(1):86-95. 2003.

Matthew J. H. et al, Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping, BMC Genomics, 9 (80): 1471-2164. 2008.

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Published

2014-06-20

How to Cite

Abdelhamid, K., Chaabane, R., M’barek, B. N., Sihem, R., Elaiche, A., & Kezan, K. (2014). Determination of the Effect of the Environment on the Genetic Polymorphism In the Genus of Tamarix Using the Molecular Marker ( Simple Sequence Repeats "PCR-SSR" (In Arid Areas of the Khenchela Region (Eastern of Algeria). International Journal of Sciences: Basic and Applied Research (IJSBAR), 16(2), 1–10. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/2121

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