Assessment of B. subtilis Toxicity in Wistar Rats through the Intraperitoneal Route
Keywords:
Entomopathogens, Zonocerus variegatus, Bacillus subtilisAbstract
The effect of exposing wistar rats to insecticidal strains of B. subtilis was evaluated in this study to determine the safety of the strain as a biocontrol agent. Z. variegatus were collected from the university agricultural fields with the aid of an insect sweep net, brought to the lab, allowed to acclimatize and watched for the onset of diseased symptoms. The entomopathogenic bacteria was isolated from diseased Zonocerus variegatus and entomopathogenicity was confirmed through reinfection of healthy batch. Diseased Zonocerus variegatus were removed for microbial analysis and B. subtilis was fingered as the main entomopathogen. The microbe was cultured in liquid media, allowed to sporulate, centrifuged and washed with saline buffer. The optimal dose of the bacteria was prepared and introduced into the experimental animals through the intraperitoneal route. Animals were watched for five days before the tissues and organs were taken for hematological and histopathological analysis. Histopathological analysis showed benign effect of the microbe on the experimental animals – a further testimony to the assertion that most entomopathogenic microbes are non-lethal to other non-target animals within the environment thus making B. subtilis a potential candidate for the preparation of microbial pesticides.
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