Butyrate Induces Production of Heat Shock Protein 27, 70 and 90 and Protects Against Carbon Tetrachloride Hepatotoxicity in Rats


  • Assa Yohana Pan African University, Life and Earth Sciences Institute (Including Health and Agriculture) (PAULESI), University of Ibadan, Ibadan Nigeria
  • Joshua Joseph Malago Department of Veterinary Anatomy and Pathology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P.O. Box 3203, Morogoro, Tanzania


Carbon tetrachloride, Butyrate, Heat shock proteins, Protection, Rats


The liver plays important roles in the body including blood cell formation, metabolism of carbohydrates, proteins and lipids, detoxification, bile production and excretion, and hepatic regeneration. It is however prone to various hepatotoxins that cause damage and diseases including consumption of alcohol whose toxicity is at an alarmingly increasing prevalence globally. Since both butyrate and heat shock proteins (Hsps) can protect various body systems against several perturbations, we investigated whether butyrate protects the liver against carbon tetrachloride (CCl4) hepatotoxicity via production of Hsps. Rats were treated with sodium butyrate (SB) for 8 days then CCl4 on the 8th day and sacrificed 1 to 2 days later. Sacrificed animals were autopsied, liver samples taken, fixed in formalin and routinely processed. Tissue sections were stained with haematoxylin and eosin or anti-Hsp90, Hsp70 and Hsp27 monoclonal antibodies to assess morphological changes and expression of Hsps. Pretreatment with SB reduced the severity of CCl4 induced hepatotoxicity which was associated with expression of Hsp90, 70 and 27. It is concluded that the protective potency of butyrate against CCl4 hepatotoxicity is mediated, at least in part, through overexpression of Hsps.


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How to Cite

Yohana, A., & Joshua Joseph Malago. (2023). Butyrate Induces Production of Heat Shock Protein 27, 70 and 90 and Protects Against Carbon Tetrachloride Hepatotoxicity in Rats. International Journal of Sciences: Basic and Applied Research (IJSBAR), 71(1), 14–30. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/16138