Butyrate Induces Production of Heat Shock Protein 27, 70 and 90 and Protects Against Carbon Tetrachloride Hepatotoxicity in Rats
Keywords:
Carbon tetrachloride, Butyrate, Heat shock proteins, Protection, RatsAbstract
The liver plays important roles in the body including blood cell formation, metabolism of carbohydrates, proteins and lipids, detoxification, bile production and excretion, and hepatic regeneration. It is however prone to various hepatotoxins that cause damage and diseases including consumption of alcohol whose toxicity is at an alarmingly increasing prevalence globally. Since both butyrate and heat shock proteins (Hsps) can protect various body systems against several perturbations, we investigated whether butyrate protects the liver against carbon tetrachloride (CCl4) hepatotoxicity via production of Hsps. Rats were treated with sodium butyrate (SB) for 8 days then CCl4 on the 8th day and sacrificed 1 to 2 days later. Sacrificed animals were autopsied, liver samples taken, fixed in formalin and routinely processed. Tissue sections were stained with haematoxylin and eosin or anti-Hsp90, Hsp70 and Hsp27 monoclonal antibodies to assess morphological changes and expression of Hsps. Pretreatment with SB reduced the severity of CCl4 induced hepatotoxicity which was associated with expression of Hsp90, 70 and 27. It is concluded that the protective potency of butyrate against CCl4 hepatotoxicity is mediated, at least in part, through overexpression of Hsps.
References
. M. Wang, J. Niu, L. Ou, B. Deng, Y. Wang and S. Li. “Zerumbone Protects against Carbon Tetrachloride (CCl4)-Induced Acute Liver Injury in Mice via Inhibiting Oxidative Stress and the Inflammatory Response: Involving the TLR4/NF-_B/COX-2 Pathway.” Molecules, vol. 24, pp. 1964, May. 2019.
. S.E. Allen. The liver: Anatomy, Physiology, Disease treatment. USA: North Eastern University Press, USA. 2002.
. K.L. Moore, A.F. Dalley. Clinically oriented Anatomy. Lippincott: Williams and Wilkins, pp. 09.
. C. Eipel, K. Abshagen, B. Vollimar. “Regulation of Hepatic Blood Flow: The Hepatic External Buffer Response Revisited.” World Journal of Gastroenterology, vol. 16, pp. 6046-6057, Dec. 2010
. M. Thompson, Y. Jaiswal, I. Wang, L. Williams. “Hepatotoxicity; Treatment, Causes and Application of Medicinal Plants as Therapeutic Agents.” The Journal of Phytopharmacology, vol. 6, pp. 186-193, Jul. 2017.
. Y. L. Wu, X. E. Peng, Y. B. Zhu, X. L. Cheo, X. Lin. “Immunotoxicology: Role of Inflammation in Chemical-induced Hepatotoxicity.” International Jounal of Virology, vol. 90, pp. 1729-1740. Jan. 2016.
. M. Luster, P. Simeonova, R. Gallucci, J. Matheson, B. Yucesoy. “Immunotoxicology: Role of Inflammation in Chemical-induced Hepatotoxicity.” International Journal of Immunopharmacology, vol. 22, pp. 1143-1147, Dec. 2000
. A. Khadir, S. Kavalakatt, P. Cherian, S. Warsame, J.A. Abubaker, M. Dehbi, A. Tiss. “Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese.” Frontier Endocrinology, vol. 9, pp. 16, Feb. 2018.
. J.J. Malago, J.F.J.G. Koninkx, J.E. van Dijk. “The hHeat Shock Response and Cytoprotection of the 1Intestinal Epithelium.” Cell Stress Chaperones, vol. 7, pp. 191-199, Apr. 2002.
. J. J. Malago, J. F. J. G. Koninkx, P. C. J. Tooten, E. A. van Liere, J. E. van Dijk. “Anti-inflammatory Properties of Heat Shock Protein 70 and Butyrate on Salmonella-induced Interleukin-8 Secretion in Enterocyte-like Caco2 Cells.” British Society for Immunology, Clinical and Experimental Immunology, vol. 141, pp. 62-71, Jul. 2005.
. M. Åkerfelt, R. Morimoto, L. Sistonen. “Heat Shock Factors: Integrators of Cell Stress, Development and Lifespan”. Nature Review for Molecular Cell Biology, vol. 11, pp. 545–555, Aug. 2010.
. S. Bellin, F. Barutta, R. Mastrocola, L. Imperatore, G. Bruno. “Heat Shock Proteins in Vascular Diabetic Complications; Review and future response.” Inetnational Journal of Molecular science. Vol. 18, Pp. 2709, Dec. 2017.
. H. H. Kampinga, J. Hageman, M.J. Vos, H. Kubota, R.M. Tanguay, et al. “Guidelines for the Nomenclature of the Human Heat Shock Proteins.” Cell Stress Chaperones, vol. 14. Pp. 105-111, Jul. 2008.
. D.D. Mosser, A.W. Caron, L. Bourget, A.B. Meriin, M.Y. Sherman, R.I. Morimoto, et al. “The Chaperone Function of hsp70 is Required for Protection Against Stress-induced Apoptosis.” Molecular Cell Biology, vol. 20, pp. 7146-7159, Oct. 2000.
. M. Ahn, J. Kim, H. Bang, J. Moon, G.O. Kim, T. Shin. “Hepatoprotective Effects of Allyl Isothiocyanate Against Carbon Tetrachloride-induced Hepatotoxicity in Rat.” Chemico-Biological Interactions, vol. 254, pp. 102-108, Jul. 2016.
. R. Mohi-ud-din, R. Hassan, G. Sawney, M. AkbarDar, Z.A. Bhat. “Possible Pathways of Hepatotoxicity Caused by Chemical Agents.” Current Drug Metabolism, vol. 20, pp. 867-879, 2019.
. A. Mir, F. Anjum, N. Riaz, H. Iqbal, H.M. Wahedi, et al. “Carbon Tetrachloride (CCl4)-induced Hepatotoxicity in Rats: Curative Role of Solanum nigrum.” Journal of Medicinal Plant Research, vol. 4, pp. 2525-2532, Jan. 201I.
. A.O. Adekeye, C.O. Akintayo, O. Adewale, S. Sabiu, A. Onikani. “Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Experimental Sprague Dawley Rats: Antioxidant Potential of Xylopia aethiopica.” Journa of Phytopharmacology, vol.3, pp. 118-123, Apr. 2014.
. W.Q. Zhang, H. Song. “Sweirtiamarin Attenuates Carbon Tetrachloride Induced Liver Injury by Regulating TLR4 Signaling Pathway.” Brazilian Journal of Pharmaceutical Sciences. Vol. 54, pp. 17449, Apr. 2018.
. R.B. Canani, M.D. Costanzo, L. Leone, M. Pedata, R. Meli, A. Calignano. “Potential Beneficial Effects of Butyrate in Intestinal and Extraintestinal Diseases.” World Journal of Gastroenterology, vol. 17, pp.1519-1528, Mar. 2011.
. D. Zhou, Q. Pan, F.Z. Xin, R.N. Zhang, C.X. He, et al. “Sodium Butyrate Attenuates High-fat diet-induced Steatohepatitis in Mice by Improving Gut Microbiota and Gastrointestinal Barrier.” World Journal of Gastroenterology, vol. 23, pp. 60-75, Jan. 2017.
. D. Zhou, Y. Chen, Z. Zhao, R. Yang, F. Xin, et al. “Sodium Butyrate Reduces High-fat diet-induced Non-alcoholic Steatohepatitis through Upregulation of Hepatic GLP-1R Expression.” Experimental and Molecular Medicine, vol. 50, pp. 1-12, Dec. 2018.
. C. Pirozzi, A. Lama, G. Cavaliere, C. Decaro, R. Citiano, et al. “Butyrate Prevents Valproate Induced Liver Injury; Invitro and Invivo Evidence.” The FASEB Journal, vol. 34, pp. 676-690, Jan. 202.
. B. Hinnebusch, S, Meng, J. Wu, S. Archer, R. Hodin. “The Effects of Short-chain Fatty Acids on Human Colon Cancer Cell Phenotype are Associated with Histone Hyperacetylation.” Jounal of Nutrition, vol. 132, pp. 1012-1017, May. 2002.
. R.A. Khan, M.R. Khan, S. Sahreen. “CCl4-induced Hepatotoxicity: Protective Effect of Rutin on p53, CYP2E1 and the Antioxidative Status in Rat.” BMC Complementally and Altenative Medicine, vol. 12 pp. 178, Oct. 2012
. N. Hamdy, E. El-Demerdash. “New Therapeutic Aspect for Carvedilol: Antifibrotic Effects of Carvedilol in Chronic Carbon Tetrachloride-induced Liver Damage.” Toxicological and Applied. Pharmacology, vol. 261 pp. 292- 299, Jun. 2012.
. T.W. Kim, J.H. Lim, I.B. Song, S.J. Park, J.W. Yang, J.C. Shin, et al. “Hepatoprotective and Antihepatitis C viral activity of Platycodon grandiflorum Extract on Carbon Tetrachloride-induced Acute Hepatic Injury in Mice.” Journal of Nutritional Science and Vitaminology, vol. 58 pp. 187-194, 2012
. J. Sun, Q. Wu, H. Sun, Y. Qiao. “Inhibition of Histone Deacetylase by Butyrate Protects Rat Liver from Ischemic Reperfusion Injury.” International Journal of Molecular Science, vol. 15, pp. 21069-21079, Nov. 2014.
. Q. J. Luo, M. X. Sun, Y. W. Guo, S. W. Tan, X. Y. Wu et al. “Sodium Butyrate Protects against Lipopolysaccharide-induced Liver Injury Partially via the GPR43/ ?-arrestin-2/NF-?B Network. Gastroenterology Report, vol. 9, pp. 154-165, Nov. 2020.
. J. J. Malago. “Over-expression of Heat Shock Protein 90 Reduces the Severity of Carbon Tetrachloride Hepatotoxicity in Rats.” International Journal of Sciences: Basic and Applied Research (IJSBAR). Vol. 38, pp. 172–188, May. 2018.
. Y. Kawashima, T. Hisaka, H. Horiuchi, H. Ishikawa, S. Uchida, et al. “The Organo- and Cytoprotective Effects of Heat-shock Protein in Response to Injury Due to Radiofrequency Ablation in Rat Liver.” Anticancer Research, vol. 36. pp. 3591-3597, Jul. 2016.
. K. Levada, N. Guldiken, X. Zhang, G. Vella, F. R. Mo et al. “Hsp72 Protects against Liver Injury via Attenuation of Hepatocellular Death, Oxidative Stress, and JNK Signaling.” Journal of Hepatolology, vol. 68, pp. 996-1005, May. 2018.
. S. Kuboki, R. Schuster, J. Blanchard, T. A. Pritts, H. R. Wong, et al. “Role of Heat Shock Protein 70 in Hepatic Ischemia-reperfusion Injury in Mice.” American Journal of Physiology Gastrointestinal and Liver Physiology, vol. 292, pp. 1141-1149, Apr. 2007.
. Y. L. Qiao, J. M. Qian, F. R. Wang, Z. Y. Ma, Q. W. Wang. “Butyrate Protects Liver against Ischemia Reperfusion Injury by Inhibiting Nuclear Factor Kappa B Activation in Kupffer Cells.” Journal of Surgical Research, Vol. 187. Pp.653-659, Apr. 2014.
. B. Lu, L. Yin, L. Xu, J. Peng. “Application of Proteomic and Bioinformatics Techniques for Studying the Hepatoprotective Effect of Dioscin against CCl?-induced Liver Damage in Mice.” Planta Medica, vol. 77 pp. 407-415, Mar. 2011
. P.K. Leong, N. Chen, P.Y. Chiu, H.Y. Leung, C.W. Ma, Q.T. Tang, et al. “Long-term Treatment with Shengmai San-derived Herbal Supplement (Wei Kang Su) Enhancesantioxidant Response in Various Tissues of Rats with Protection against Carbontetrachloride Hepatotoxicity.” Journal of Medicinal Food, vol. 13 pp. 427-438, Apr. 2010.
. H. Yamamoto, Y. Yamamoto, K. Yamagami, M. Kume, S. Kimoto, S. Toyokuni, et al. “Heat-shock Preconditioning Reduces Oxidative Drotein denaturation and Ameliorates Liver Injury by Carbon Tetrachloride in Rats.” Research in Experimental Medicine (Berl), vol. 199 pp. 309-318, Jun. 2000
. R. M. Ahmed and A. K. Mohamme. “Amelioration of Hepatotoxicity by Sodium Butyrate Administration in Rats.” World’s Veterinary Journal, vol. 12, pp. 323-329, Sept. 2022.
. A. Sarkar, P. Mitra, A. Lahiri, et al. “Butyrate Limits Inflammatory Macrophage Niche in NASH.” Cell Death and Disease, vol. 14, pp. 332, May. 2023.
. X. Luo, X. Zuo, Y. Zhou, B. Zhang, Y. Shie et al. ‘Extracellular Heat Shock Protein 70 Inhibits Tumour Necrosis Factor-alpha Induced Proinflammatory Mediator Production in Fibroblast-like Synoviocytes.” Arthritis Research Therapy, vol. 10, R41, Apr. 2008.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 International Journal of Sciences: Basic and Applied Research (IJSBAR)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who submit papers with this journal agree to the following terms.
