Fresh and Frozen Semen Quality of Horned and Polled Bali Bulls

Authors

  • Suharni Suharni Study Program of Animal Science and Technology, Graduated School of Hasanuddin University, Jl. Perintis Kemerdekaan KM.10, Makassar 90245, Indonesia
  • Herry Sonjaya Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Jl. Perintis Kemerdekaan Km.10, Makassar 90245, Indonesia
  • Hasbi Hasbi Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Jl. Perintis Kemerdekaan Km.10, Makassar 90245, Indonesia
  • siti farida Livestock and Animal Health Service of South Sulawesi Province, Jl. Veteran Selatan No. 234 Makassar 90131, Indonesia

Keywords:

Horned Bali Bulls, Fresh Semen, Frozen Semen, Semen Quality, Polled

Abstract

Semen quality is an important factor in the success of artificial insemination (AI). This study aims to determine the quality of fresh and frozen semen from horned and polled Bali bulls. This research was conducted at the Regional Artificial Insemination Center (RAIC) of South Sulawesi and the In Vitro Embryo Production Laboratory, Faculty of Animal Husbandry, Hasanuddin University. The research materials were the semen of two horned Bali bulls and two polled Bali bulls, each 5-6 years old. Parameters observed in fresh semen include volume, pH, color, concentration, motility, viability, abnormality, and an intact plasma membrane. While the parameters in frozen semen include motility, viability, abnormality, an intact plasma membrane, acrosome integrity, and DNA integrity. Viability and abnormality were tested using eosin-nigrosin, intact plasma membrane was tested using hypoosmotic swelling (HOS), acrosome integrity was tested using formal saline, and DNA integrity was tested using acridine orange (AO). The data obtained were tested with a T-test (Independent sample test). The results showed that there was no significant difference (P > 0.05) between fresh and frozen semen of polled Bali bulls and horned Bali bulls on all semen quality parameters. The conclusion is that fresh and frozen semen from polled and horned Bali bulls have the same quality.

References

BPS. 2021. Badan Pusat Statistik. Indonesia.

Ministry of Agriculture of the Republic of Indonesia. 2016. Peraturan Menteri Pertanian Republik Indonesia Nomor 10/Permentan/PK.210/3/2016. Kementerian Hukum dan Hak Asasi Manusia Republik Indonesia. Jakarta.

Susilawati, T. 2011. Spermatologi. Universitas Brawijaya (UB) Press. Malang.

Handayani, E., Supriatna, I., Tumbelaka, L. I., & Kaiin, E. M. 2021. “J Vet” Comparative analyzis of quality sni certified and non sni certified frozen semen. Vol 2(2), pp. 207-15.

Zulkarnain. 2017. Studi Karakteristik Sapi Bali Polled Sebagai Sapi Lokal Di Sulawesi Selatan. Disertasi. Sekolah Pascasarjana. Universitas Hasanuddin. Makassar.

Cargill, E. J., Nissing, N. J and Grosz, M. D. 2008. Single Nucleotide Polymorphisms Concordant with The Horned/Polled Trait InHolsteins. BMC Research Notes. Vol 9(1),pp.1–9.

Baco, S., Zulkharnain., Malaka, R and Gozali R. Moekti. 2020. Polled Bali Cattle and Potentials for the Development of Breeding Industry in Indonesia. Hasanuddin Journal Animal Science. Vol 2(1), pp. 23-33.

Hasbi, H., Prahesti K. I., Sonjaya, H., Baco, S., Wildayanti, W and Gustina, S. 2021. Characteristics of Libido And Testosterone Concentrations Of Bali Polled And Horned Bulls. IOP Conf Ser Earth Environ Science 788:012141. DOI: 10.1088/1755-1315/788/1/012141.

Garner, D. L and Hafez, E. S. E. 2008. Spermatozoa and Seminal Plasma in Reproduction in Farm Animals 7th Edition. Ed by Hafez ESE, Lea and Febiger. Phladelphia: Vol. 96-110.

Priyanto, L., Arifiantini, R.I. and Yusuf, T.L. 2015. Detection of sperm DNA damage in fresh and frozen semen using Toluidine Blue Staining. J. Vet. Vol 16(1), pp. 48-55.

Adiputra, K.D.D., Maulana, T., Kaiin, E.M., Hasbi, H and Sonjaya, H. 2022. The Semen Quality Of Bali And Simmental Bulls Reared In Technical Implementation Unit Of Regional Artificial Insemination Center At Pucak, South Sulawesi. Adv. Anim. Vet. Vol 10 (12), pp. 2562-2570.

Centola, G. M. 2018. Semen Analisys. In. Skinner, M. K (ed). Encyclopedia of Reproduction. Publisher Elsevier Science Publishing Co Inc. USA. Vol. 8

Nova, Y., Karja, N. W. K dan Arifiantini, R. I. 2017. Status Akrosom dan Kualitas Post-Thawed Spermatozoa pada Beberapa Rumpun Sapi dari Dua Balai Inseminasi Buatan. Acta Veterinaria Indonesian. 5(2): 81-88.

Adiputra, K.D.D., Sukandi, S., Farida, S., Sonjaya, H and Hasbi, H. 2023. Progressive Motility, DNA Fragmentation, Intact Plasma Membrane, and Acrosome Status of Frozen Semen Bali and Simmental Bulls. Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Jl. Perintis Kemerdekaan Km.10, Makassar, Indonesia. Hasanuddin J. Anim. Vol 4(2), pp.109-118.

Anwar, P., Ondho, Y. S dan Samsudewa D. 2015. Kualitas Membran Plasma Utuh dan Tudung Akrosom Utuh Spermatozoa Sapi Bali Dipreservasi Suhu 5°C dalam Pengencer Ekstrak Air Tebu dengan Penambahan Kuning Telur. Agromedia. Vol.33(1), pp. 53-63.

Sugiarti T., Triwulanningsih, E., Situmorang, P., Sianturi, R. G dan Kusumaningrum, D. A. 2004. Penggunaan katalase dalam produksi semen dingin sapi. Seminar Nasional Teknologi Peternakan dan Veteriner. 215-220.

Samsudewa, D., Wuwuh, M. I. S dan Ondho, Y. S. 2007. Pengaruh jumlah spermatozoa per inseminasi terhadap kualitas semen beku kambing peranakan Etawa. Seminar Nasional Teknologi Peternakan dan Veteriner. 462-468.

Cahya, R.I., Ondho, Y.S dan Setiatin, E.T. 2017. Persentase Membran Plasma Utuh dan Tudung Akrosom Utuh Spermatozoa Kambing Peranakan Etawah dalam Pengencer yang Berbeda. Fakultas Peternakan dan Pertanian. Universitas Diponegoro. Semarang.

Prabowo T. A., Arifiantini, R. I., Sajuthi, D dan Saefullah, U. 2016. Pengembangan metode identifikasi kerusakan DNA spermatozoa ternak. Jurnal Sain Veteriner. Vol. 32(2), pp. 166-171.

Evenson, D. P. 2016. The Sperm Chromatin Structure Assay (SCSA®) and Other Sperm DNA Fragmentation Tests for Evaluation of Sperm Nuclear DNA Integrity as Related to Fertility. Animal Reproduction Science. 169: 56-75.

Nursyam. 2007. Perkembangan Iptek Bidang Reproduksi Ternak untuk Meningkatkan Produktivitas Ternak. JITV. Vol. 21 (4), pp. 145-152.

Feradis. 2010. Bioteknologi Reproduksi pada Ternak. Alfabeta. Bandung.

Ismaya. 2014. Bioteknologi Inseminasi Buatan pada Sapi dan Kerbau. Gadjah Mada University Press. Yogyakarta.

Indriani., Susilawati, T dan Wahyuningsih, S. 2013. Daya Hidup Spermatozoa Sapi Limousin yang Dipreservasi dengan Metode Water Jacket dan Proses Pembekuan. Buletin Peternakan Vol. 37(3), pp.143-147.

Sundari, T.W., Tagama, T.R dan Maidaswar. 2013. Kolerasi kadar pH semen segar dengan kualitas semen sapi limousine di Balai Inseminasi Buatan Lembang. Fakultas Peternakan Universitas Jenderal Soedirman. Purwokerto. Jurnal Ilmiah Peternakan. Vol 4(2. pp109-118.

Ax,R. L., Dally, M., Didion, B. A., Lenz, R. W., Love, C. C., Varner, D. D., Hafez, B and Bellin, M. E. 2008. Semen Evaluation in Farm Animal Reproduction ed By Hafez ESE. 7th Lea Febiger: 365-375.

Diansyah A. M, M. Yusuf A. L. Toleng, M. I. A. Dagong. (2022). Characteristic and Kinematics of Bali-Polled Bull Sperms. Adv. Anim. Vet. Sci. 10(8): 1787-1796.

Gustina, S., Hasbi, H., Sonjaya, H., Baco, S., Toleng, A.L., Mutmainna, M and Farida, S. “JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis)” . Motility and Viability of Spermatozoa of Polled and Horned Bali Bulls at Each Stage of the Freezing Process. Vol 9(1):48-54. 2021

Sunami, S., N. Isnaini, dan S. Wahjuningsih. 2017. Kualitas semen segar dan recovery rate (RR) sapi Limousin pada musim yang berbeda. Journal of Tropical Animal Production. Vol.18(1), pp. 36-50.

Dewi, A.S., Y. S. Ondho, dan E. Kurnianto. (2012). Kualitas Semen Berdasarkan Umur Pada Sapi Jantan Jawa. Animal Agriculture Journal, Vol. 1 (2), pp. 126 – 133.

Wahyuningsih A., D.M. Saleh dan Sugiyatno. (2013). Effect of male age and storage frequency on volume and motility of fresh semen of Simmental cattle at the Lembang Artificial Insemination Center. J. Ilmiah Peternakan. Vol.1(3), pp. 947-953.

Sarastina., Susilawati, T dan Ciptadi, G. 2006. Analisa Beberapa Parameter Motilitas Spermatozoa Pada Berbagai Bangsa Sapi Menggunakan Computer Assisted Semen Analysis (Casa). Jurnal Ternak Tropika.Vol. 6 (2), pp.1-12.

SNI 4869-1. 2017. Semen Beku – Bagian 1: Sapi. Badan Standar Nasional Indonesia. Jakarta.

Ducha, N., T. Susilawati., Aulanni’am dan S. Wahjuningsih. 2013. Motilitas dan Viabilitas Spermatozoa Sapi Limosin Selama Penyimpanan pada Refrigator dalam Pengencer CEP-2 dengan Suplementasi Kuning Telur. Jurnal Kedokteran Hewan.7(1):5-8.

Haryani, R., Latief, A., Sonjaya, H., & Yusuf, M. 2016. Characteristic of Bali Bulls Sperms Assessed Using Computerized Assisted Semen Analysis ( CASA ). International Journal of Sciences: Basic and Applied Research. 4531: 161–168.

Arsiwan, A., T. Saili, L.A. Ba'a, & S. Rahadi. 2014. Membran plasma utuh sprmatozoa epididimis kambing peranakan ettawa dalam natrium klorida dengan konsentrasi berbeda. Jitro. Vol. 1(1), pp. 79-87.

Rahmiati R., Eriani K., Dasrul D. (2018). Kualitas dan morfologi abnormal spermatozoa sapi aceh pada berbagai frekuensi ejakulasi. Prosiding Biotik, 3(1).

Rizal, M. “Jurnal Pengembangan Peternakan Tropis” Pengaruh Penambahan Laktosa Di Dalam Pengencer Tris Terhadap Kualitas Semen Cair Domba Garut. Vol 31 (1), pp 224-231. 2006

Fadillah, N. 2022. Membran Plasma Utuh (Mpu) Semen Segar Dan Semen Beku Sapi Bali Polled Dan Sapi Bali Bertanduk. Fakultas Peternakan

Universitas Hasanuddin Makassar. Indonesia.

Peris-Frau, P., A.J. Soler, M. Iniesta-Cuerda, A. Martín-Maestro, I. Sánchez-Ajofrín, D.A. Medina-Chávez, & et al. “Int J Mol”. Sperm cryodamage in ruminants: understanding the molecular changes induced by the cryopreservation process to optimize sperm quality.Vol 21(8). pp 2781. 2020.

Ozkavukcu, S., Erdemli, E., Isik, A., Oztuna, D and Karahuseyinoglu, S. “Journal of Assisted Reproduction Genetic”. Effects of Cryopreservation on Sperm Parameters and Ultrastructural Morphology of Human Spermatozoa. Vol 2(5), Pp. 403-411. 2020.

Kim HS, Kang MJ, Kim SA, Oh SK, Kim H, Yup Ku S, Kim SH, Moon SY, Choi YM. “Clin Exp Reprod Med” The utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis. Vol 40(1), Pp. 23-28. 2013.

Hasbi, H., Dagong, M.I.A., Zulkharnain, Z., Baba, S., Sonjaya, H., Baco, S., Gustina, S., Maulana, T., Gunawan, M., Agung, P.P., Herlina, N., Yanthi, N.D., Kaiin, E.M and Said, S. 2023. Comparison of Fresh and Cryopreserved Semen Quality of Polled and Horned Bali Bulls. Faculty of Animal Science, Hasanuddin University, South Sulawesi, Indonesia. Iranian Journal of Applied Animal Scienc2 Vol. 13(1), pp. 33-41.

Uswa, H.M., Sonjaya, H., Gustina, S and Hasbi, H. 2022. The Concentration of Hydrogen Peroxide, Percentage of Motility, Viability, Plasma Membrane Integrity, and Abnormality of Frozen Semen of Horned and Polled Bali . International Journal of Sciences: Basic and Applied Research (IJSBAR) (2022) Vol. 64 (1) 1, pp 104-115.

Suyadi. 2001. Pengaruh suhu dan waktu penyimpanan semen beku sapi FH Post Thawing terhadap Kualitas Sperma Post Kapasitasi. J.Tropical animal.2001. Vol. 85-90.

India Agriculture Ministry. (2014). Compendium of Minimum Standards of Protocol and Standard Operating Procedures for Bovine Breeding. Department of Animal Husbandry, Dairying and Fisheries, India.

Arifiantini, R. I dan Yusuf, T. L. 2012. Keberhasilan Penggunaan Tiga Pengencerdalam Dua Jenis Kemasan pada Proses Pembekuan Semen Sapi Frisien Holstein. Jurnal Peternakan. 9(3). Fakultas Kedokteran Hewan. Institut Pertanian Bogor.

Prinosilova, P., Rybar, A., Zajicova, A dan Hlavicova, J. 2012. Integritas DNA pada Spermatozoa Anjing Segar, Dingin dan Beku. Lembaga Penelitian Veteriner. Veterinaria Medicina. Vol. 57(3), pp133-142.

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Published

2023-04-24

How to Cite

Suharni, S., Sonjaya, H., Hasbi, H., & farida, siti. (2023). Fresh and Frozen Semen Quality of Horned and Polled Bali Bulls. International Journal of Sciences: Basic and Applied Research (IJSBAR), 68(1), 1–17. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/15659

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