Increasing Mature Spermatids Formation Using Pasak Bumi
Keywords:
Eurycoma longifolia, Pasak bumi, endemic of Borneo, Hematoxylin Eosin, mature spermatidsAbstract
Pasak bumi as a rare plant with eroded status is benefit as aphrodisiac for Borneo men. This study investigate the effect of pasak bumi (Eurycoma longifolia) on spermatozoon cell stages development. The research was conducted by giving pasak bumi steeping doses of 18 mg/200 g (=90 mg/kg) of bodyweight (bw) for three days to 15 white male rats as the experiment group, and 15 white male rats also given with distilled water as the control group. Micromorphological analysis was carried out using histological staining of Hematoxylin Eosin (HE) in the seminiferous tubule tissue. The result showed that pasak bumi treatment until day-3 is: 1) clearly the amount of mature spermatids was increased, 2) maintaining the amount of spermatogonia, 3) meanwhile the amount of primary spermatocyte was decreased, in term of related to primary spermatocyte formation. It is concluded that pasak bumi increased mature spermatids formation.
References
Hadiah, JT., Yuzammi, and Purnomo D.W.. “Habitat and population studies of Eurycoma longifolia at the western block of conservation forest of PT Sabhantara Rawisentosa, Kutai Timur, East Kalimantan.” The Botanic gardens Bulletin, vol. 22, no.1, pp. 31-46, Jan. 2019
Adimoelja, A. “Phytochemical and the breakthrough of traditional herbs in the management of sexual dysfunction.” Int J Androl vol. 23, no 2, pp. 82-84, Jan. 2000
Ezzat, SM., Okba M., Ezzat, MI., Aborehab, NM., and Mohamed SO. “Rho-Kinase II Inhibitory Potential of Eurycoma Longifolia New Isolate for The Management of Erectile Dysfunction”. Hindawi, Evidence-Based Complementary and Alternative Medicine. Vol. 2019, pp. 1-8, May 2019.
Ang HH, Lee KL, Kiyoshi M. “Sexual arousal in sexually sluggish old male rats after oral administration of Eurycoma longifolia Jack - tongkat Ali [Abstract]”. J Basic Clin Physiol Pharmacol, vol 15. no 3-4, pp. 303-9, Jan. 2004.
Iriawati, Rahmawatia, A., Rizkita R. Esyantia. “Analysis of Secondary Metabolite Production in Somatic Embryo of Pasak Bumi (Eurycoma longifolia Jack.)”. Procedia Chemistry, vol. 13, pp. 112 – 118, March 2014 DOI: 10.1016/j.proche.2014.12.014
Pratomo, H., I. SupriatnaI, W. Manalu, dan A. Winarto A. “Perubahan Sebaran Sel-Sel Asidofil dan Basofil Hipofisa Pengaruh Pemberian Pasak Bumi (Eurycoma longifolia Jack)”. J. Matematika, Sains, dan Teknologi. Vol. 12, no. 2. pp. 80-91, July 2011 [Indonesian]
Panjaitan Ruqiah GP. “Pengujian aktivitas hepatoprotektor akar pasak bumi (Eurycoma longifolia Jack)” Doctor dissertation, Sekolah Pascasarjana IPB., Bogor, Indonesia, 2008 [Indonesian]
Lu FC. Toksikologi Dasar, Asas, Organ sasaran, dan penilaian resiko. Ed 4th . Nugroho E, translator; of Basic toxicology: Fundamentals, target organs, and risk assessment. Jakarta: UI Press, 1995, pp. 85-105 [Indonesian]
Pratomo, H. “Effects of Eurycoma longifolia provision on Blood sugar level, cholesterols, and uric acid of Etawa Crossbreed Goat.” Journal of Physics: Conf. Series 102 5012069, vol. 2018, pp. 1-9, Jan. 2018
Pratomo, H. “Eurycoma longifolia extract increases intracellular production activity of luteinizing hormone (LH) in pituitary”. In AIP Conference Proceedings, 1862. Issue 1. 10.1063/1.4991216. 2017, pp. 030112-1-6. https://doi.org/10.1063/1.4991216
Low, B.-S., Choi S-B, H. Abdul Wahab, P. Kumar Das, and K.-L. Chan. “Eurycomanone, the major quassinoid in Eurycoma longifolia root extract increases spermatogenesis by inhibiting the activity of phosphodiesterase and aromatase in steroidogenesis”. Journal of Ethnopharmacology, vol. 149, no.1, pp. 201–207, June 2013.
Bhat, R. and Karim, AA. “Tongkat Ali (Eurycoma longifolia Jack) a review on its etnobotany and pharmacological importance”. Fitoterapia vol 81, no. 7, pp. 669-679, April 2010
Ezzat, Shahira M, Mona M. Okba, Marwa, Ezzat, Nora M. Aborehab, and Shanaz O. Mohamed. “Rho-Kinase II Inhibitory Potential of Eurycoma longifolia New Isolate for the Management of Erectile Dysfunction.“ Evid Based Complement Alternat Med vol. 2019 | Article ID 4341592, pp. 1-8, May 2019. https://doi.org/10.1155/2019/4341592
Pratomo, H., I. Supriatna, W. Manalu, dan A. Winarto. “Tingkat aktifitas sel endokrin penghasil folikel stimulating hormon (FSH) terkait pemberian pasak bumi (Eurycoma longifolia Jack).” J. Kefarmasian Indonesia, vol. 1, no 1, pp.1-10, Feb. 2012. DOI:10.22435/JKI.V2I1.4038.1-10 [Indonesian]
Pratomo, H. “Evaluation of The Effect of Pasak Bumi (Eurycoma longifolia Jack) to Increase Androgen Levels”. International Series on Interdisciplinary Research (INSIST), vol. 1, no 1, pp. 1-7, Oct 2016, available: https://garuda.kemdikbud.go.id/documents/detail/959008. ISSN Online: 2502-8588.
Fayig EM, Foad Qaw. “Serum and plasma levels of total and free testosterone and of sex hormone binding globulins in rats growing in the below sea level environtment of the Jordan valley”. The Internet Journal of Endocrinology vol. 5, no. 2, pp. 1-6, Jan. 2008, available: https://ispub.com/IJEN
Barranquero M.G. “Role of LH in spermatogenesis.” Invitra. Certified Medical Magazine by WMA, ACSA, HON., Jan. 2023. available: https://www.invitra.com/en/lh/hormone-regulation-spermatogenesis-lh-testosterone/
Krisfalosi M, Vivoshi M, Patricia LM, Deborah AD.. “Multiple glycolitic enzymes are tighly bound to the fibrous sheath of rat spermatozoon.” Biol Reprod . vol. 75, pp. 270-278, May 2006.
Andarini M, “Standardisasi obat tradisional dan tantangan dalam masa pandemi covid-19” Disampaikan pada Webinar Trop BRC Talk Series-7, August 18 2020. Available: http://biofarmaka.ipb.ac.id/biofarmaka/2020/Materi%20Webinar%20TropBRC%20%237%20-%20Dra.%20Rr.%20Mayagustina%20Andarini,%20Apt.,%20MSc..pdf . [Indonesian]
Pratomo H. “Efek rimpang kunyit (Curcuma domestica Val) sebagai anti piretik pada tikus putih jantan yang didemamkan”, undergraduate thesis, Fakultas Biologi UNAS, Jakarta, Indonesia. 1987 [Indonesian]
Kiernan J.A. Histological and Histochemical Methods: Theory and Practice. 5th edition, England, Scion Publishing, 2015, 571 pp
Fawcett, DW. “A comparative view of sperm ultrastructure.” Biol. Reprod vol.2 Suppl 2, pp. 90-127. Jan. 1970
Samsul, H. “Penambatan Molekul Kandungan Eurycoma longifolia Jack. (Pasak Bumi) terhadap Human Phosphodiesterase 5.” Repo Dosen ULM. https://repo-dosen.ulm.ac.id//handle/123456789/18463. pp 1-2, Jan. 2020. (accesed: Jan 17th 2023) [Indonesian].
Abubakar BM, Mohd SF, Omar MSS, Wagiran A. “DNA barcoding and chromatography finger prints for the authentication of botanicals in herbal medicinal products.” Evid Based Compl Altern Med vol. 2017, pp. 1-9, April 2017. https://doi.org/10.1155/2017/1352948
Yulita Sri K, Susilowati A., Rachmat, H. H., Susila, Hidayat A., Dwiyanti, F. G. “Molecular identification of Eurycoma longifolia Jack from Sumatra, Indonesia using trnL-F region.” Biodiversitas vol. 23, No 3, pp. 1374-1382, March 2022.
Wong, C. H. and Cheng, C. Y. “The blood-testis barrier: its biology, regulation and physiological role in spermatogenesis.” Curr. Topics Dev. Biol. vol. 71, pp. 263–296, Jan. 2005
Ang HH, Hitotsuyanagi Y, Takeya K. “Eurycolactones AC, novel quassinoids from Eurycoma longifolia.” Pythochem tetrahedron vol. 41, no. 35, pp. 6849-6853, August 2000.
Debing E, Peeters E, Duquet W, Poppe K, Velkeniers B, Van Den Brande P. “Men with atherosclerotic stenosis of the carotid artery have lower testosterone levels compared with controls.” Int Angiol. vol. 27, no.2, pp.135-41, April 2008
León I, Elena R.A, Santiago M, José L A. “Shape of Testosterone.” J Phys Chem Lett.
vol. 12, no.29, pp. 6983-6987, July 2021.
Johnson MH, Essential reproduction 8th edition. London, Wiley-Blackwell publ., pp. 432, March 2018.
Cox RM and John AHB. “Testosterone has opposite effects on male growth in lizards (Sceloporus spp.) with opposite patterns of sexual size dimorphism.” J Exp Biol vol. 208, no. 24, pp. 4679–87, Sept. 2005.
Matthiesson KL, Robert IM, Liza OD, Mark F, David MR, Peter GS, and Sarah JM.. “The relative roles of FSH and LH in maintaining spermatogonial maturation and spermiation in normal men.” The J of Clin Endocrinol and Metab. vol. 91, no. 10, pp.3962-3969, August 2006.
Smith E. R., Stefanick M. L., Clark J. T., Davidson J. M. “Hormones and sexual behavior in relationship to aging in male rats” Hormones and Behavior vol. 26, Issue 1, pp 110-135, March 1992.
Pratomo, H., and Yudi. “Pemberian Pasak Bumi Memengaruhi Kadar Testosteron dan Spermatozoa Kambing Peranakan Etawa.” Jurnal Kedokteran Hewan vol. 10, no. 2, pp. 148-152. Sept. 2016. [Indonesian].
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