Macrophages Activity and Capacity of Staphylococcus Aureus-induced Male Rattus Norvegicus, Sprague Dawley Strain Subsequent to Extra Virgin Olive Oil and Honey Mixture Administration

  • Retno Mardhiati Public Health Department, Prof. Dr. Hamka Muhammadiyah University, South Jakarta, Indonesia.
  • Sri Anna Marliyati Department of Community Nutrition, IPB University, Bogor, Indonesia.
  • Drajat Martianto Department of Community Nutrition, IPB University, Bogor, Indonesia.
  • Siti Madanijah Department of Community Nutrition, IPB University, Bogor, Indonesia.
  • I Wayan T Wibawan Department of Animal Disease and Veterinary Health, IPB University, Bogor, Indonesia.
Keywords: Extra Virgin Olive Oil, Honey, macrophages, Phagocytosis


Extra virgin olive oil (EVOO) and honey as immunomodulator. Non-specific immune system is the body initial defense for infection, where macrophages are one of its parts. Activated macrophages will move more actively. This study aims to analyze the effect of EVOO and honey mixture administration on the macrophages activity and capacity of Staphylococcus aureus induced male rats (Rattus norvegicus) Sprague Dawley strain. This experimental study was conducted during May to June 2019. The total rats examined was 80, divided into 5 groups that were treated orally for 1 to 4 weeks. The groups of rats were K1 (-) Aquades, K2 (+) Stimuno, E1 EVOO, E2 honey, and E3 EVOO and honey mixture. Staphylococcus aureus were induced intraperitonally into the rats. The macrophages were examined in Bacteriology and Immunology laboratory of FKH IPB University. Macrophages tests were conducted 1 hour subsequent to bacterial induction. The analysis of difference was done using one way anova and the analysis of group difference was carried out using Duncan test. The result shows that there are macrophage capacity (P= 0.000) differences on the first week but not on the macrophage activity (P=0.079), with highest mean value of macrophage activity (62.5±3.32) and capacity (2927.3±42.3) belong to E3 group. There are significant differences of both macrophage capacity and activity on the 2nd, 3rd, and 4th week (P < 0.005).


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How to Cite
Retno Mardhiati, Marliyati, S. A., Martianto, D., Madanijah, S., & Wibawan, I. W. T. (2022). Macrophages Activity and Capacity of Staphylococcus Aureus-induced Male Rattus Norvegicus, Sprague Dawley Strain Subsequent to Extra Virgin Olive Oil and Honey Mixture Administration. International Journal of Sciences: Basic and Applied Research (IJSBAR), 62(2), 94-105. Retrieved from