Study the Effect of Locally Produced Clostridium perfringens Enterotoxin on the MCF-7 and T47D Breast Cancer Cell Lines
AbstractBreast cancer is the most common cancer among women worldwide after skin cancer. It is also the second leading cause of cancer death in women after lung cancer .One in eight women in the United States (12%) will develop breast cancer in her lifetime and this percent is in a continuous increasing .Breast cancer treatment regimens differ widely based on the type of cancer, its stage, its sensitivity to hormones, the patient’s age and health, and other factors. Surgery and radiation therapy are mainstays of breast cancer treatment as well as chemotherapy. Despite of the remarkable development in cancer treatment and new drugs ,but the progress in recovery of cancer still weak so the need to new strategies of therapy and to new drugs remains very important . One of these strategies is the gene therapy by which such strange toxic molecules specifically are directed to the cancer cells ,one of these candidates that attracting attention are the bacterial toxins which revealed high activity in killing of the cancer cells in in vitro and in vivo studies . Our study paid attention to one of the bacterial toxins to screen on its activity in this respect . In more details, we screen the activity of locally isolated Clostridium Perfringens enterotoxin on the breast cancer cell lines as a first step and for the first time locally to detect on the effect of bacterial toxins isolated from our environments as well as there is no previous local study on CPE that extracted and purified from bacteria isolated from food poisoning patients in Mosul city.
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