Study the Effect of Locally Produced Clostridium perfringens Enterotoxin on the MCF-7 and T47D Breast Cancer Cell Lines

  • Alaa Hussein Almola Department of Biology, College of Sciences, University of Mosul, Mosul-IRAQ
  • Amera Mahmmod Al-Rawi Department of Biology, College of Sciences, University of Mosul, Mosul-IRAQ
Keywords: Clostridium perfringens enterotoxin, toxin, cancer, Claudin 3, MCF-7, T47D

Abstract

Breast cancer is the most common cancer among women worldwide after skin cancer. It is also the second leading cause of cancer death in women after lung cancer .One in eight women in the United States (12%) will develop breast cancer in her lifetime and this percent is in a continuous increasing .Breast cancer treatment regimens differ widely based on the type of cancer, its stage, its sensitivity to hormones, the patient’s age and health, and other factors. Surgery and radiation therapy are mainstays of breast cancer treatment as well as chemotherapy. Despite of the remarkable development in cancer treatment and new drugs ,but the progress in recovery of cancer still weak so the need to new strategies of therapy and to new drugs remains very important . One of these strategies is the gene therapy by which such strange toxic molecules specifically are directed to the cancer cells ,one of these candidates that attracting attention are the bacterial toxins which revealed high activity in killing of the cancer cells in in vitro and in vivo studies . Our study paid attention to one of the bacterial toxins to screen on its activity in this respect . In more details, we screen the activity of locally isolated Clostridium Perfringens enterotoxin on the breast cancer cell lines as a first step and for the first time locally to detect on the effect of bacterial toxins isolated from our environments as well as there is no previous local study on CPE that extracted and purified from bacteria isolated from food poisoning patients in Mosul city.

References

S. Kominsky et al., "Clostridium perfringens Enterotoxin Elicits Rapid and Specific Cytolysis of Breast Carcinoma Cells Mediated through Tight Junction Proteins Claudin 3 and 4", The American Journal of Pathology, vol. 164, no. 5, pp. 1627-1633, 2004. Available: 10.1016/s0002-9440(10)63721-2.

J. Black, S. Lopez, E. Cocco, C. Schwab, D. English and A. Santin, "Clostridium Perfringens Enterotoxin (CPE) and CPE-Binding Domain (c-CPE) for the Detection and Treatment of Gynecologic Cancers", Toxins, vol. 7, no. 4, pp. 1116-1125, 2015. Available: 10.3390/toxins7041116.

S. HASHIMI, S. YU, N. ALQURASHI, D. IPE and M. WEI, "Immunotoxin-mediated targeting of claudin-4 inhibits the proliferation of cancer cells", International Journal of Oncology, vol. 42, no. 6, pp. 1911-1918, 2013. Available: 10.3892/ijo.2013.1881.

W. Strober, "Trypan Blue Exclusion Test of Cell Viability", Current Protocols in Immunology, vol. 111, no. 1, 2015. Available: 10.1002/0471142735.ima03bs111.

S. Kamiloglu, G. Sari, T. Ozdal and E. Capanoglu, "Guidelines for cell viability assays", Food Frontiers, vol. 1, no. 3, pp. 332-349, 2020. Available: 10.1002/fft2.44.

S. Felgner, D. Kocijancic, M. Frahm and S. Weiss, "Bacteria in Cancer Therapy: Renaissance of an Old Concept", International Journal of Microbiology, vol. 2016, pp. 1-14, 2016. Available: 10.1155/2016/8451728.

S. Kareem and A. Al-Ezee, "Food poisoning (Salmonellosis)", Research Journal of Pharmacy and Technology, vol. 13, no. 2, p. 529, 2020. Available: 10.5958/0974-360x.2020.00100.6.

J. Li, "Targeting claudins in cancer: diagnosis, prognosis and therapy". American Journal Cancer. Research 2021. vol 11, no. 7, p. 3406–3424.

M. Sedighi et al., "Therapeutic bacteria to combat cancer; current advances, challenges, and opportunities", Cancer Medicine, 2019. Available: 10.1002/cam4.2148.

Published
2022-04-25
How to Cite
Alaa Hussein Almola, & Amera Mahmmod Al-Rawi. (2022). Study the Effect of Locally Produced Clostridium perfringens Enterotoxin on the MCF-7 and T47D Breast Cancer Cell Lines. International Journal of Sciences: Basic and Applied Research (IJSBAR), 62(2), 48-56. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/13930
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Articles