The Effect of Using Soybean Extender on the Movement Patterns of Spermatozoa and Plasma Membrane Integrity in Bali Bull

Authors

  • Khusnul Khatimah Animal Technology Postgraduate Student, Faculty of Animal Science Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10 Tamalanrea, Makassar, South Sulawesi, Indonesia
  • Abdul Latief Toleng Faculty of Animal Science, Hasanuddin University, Jl. PerintisKemerdekaan Km. 10 Tamalanrea, Makassar, South Sulawesi, Indonesia
  • Muhammad Yusuf Faculty of Animal Science, Hasanuddin University, Jl. PerintisKemerdekaan Km. 10 Tamalanrea, Makassar, South Sulawesi, Indonesia

Keywords:

Soybean, Extender, Bali bull, Plasma membrane integrity (PMI), Movement pattern

Abstract

Selection of good extender quality greatly affects the quality of spermatozoa. One of the natural ingredients that can be used as a semen extender is soybean, which is known to maintain the quality of spermatozoa by preventing cold shock. This study was conducted to determine the effect of using soybean extender on the movement pattern and plasma membrane integrity (PMI) of spermatozoa. This study was conducted at the Samata Integrated Farming System (SIFS), Samata Village, Kab. Gowa. The processing of semen was carried out at the Laboratory of Animal Reproduction, Faculty of Animal Science, Hasanuddin University. A Bali bull was used to collect the semen for five times using an artificial vagina. Four types of extenders as treatments were used to extend the semen; T0 (andromed) for control, T1 (tris), T2 (soybean), and T3 (tris-soybean) The parameters measured were the plasma membrane integrity (PMI) and movement patterns of spermatozoa. The results showed that the average motility of fresh semen obtained was 94.22% ± 2.37, viability 96.06% ± 1.09, concentration 1596 x 106/mL ± 1472, and MPI 95.11% ± 1.82.

The average percentage of MPI in the use of tris soybean extender was higher (97.79 ± 1.14) compared to other extenders and there was no significant difference (P>0.05) was found between the four treatments. In the movement pattern parameters, all treatments in each parameter (VCL, VAP, VSL, DCL, DAP, DSL, LIN, and STR) did not show any significant difference to the control. Based on the results of study, it can be stated that the use of soy extender was able to maintain the quality semen and can be classified as good for further processed. As conclusion, the use of soybean extender was able to maintain the quality of Bali bull semen indicated that soybean extender was classified as very good and could be used further.

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Published

2021-12-01

How to Cite

Khusnul Khatimah, Abdul Latief Toleng, & Muhammad Yusuf. (2021). The Effect of Using Soybean Extender on the Movement Patterns of Spermatozoa and Plasma Membrane Integrity in Bali Bull. International Journal of Sciences: Basic and Applied Research (IJSBAR), 60(5), 219–227. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/13639

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