Vitamin E Attenuates Cardiomyopathy Via Alleviation of Autophagic Stress

  • Eman Farrag Lecturer of clinical Pharmacology, Faculty of Medicine Mansoura University, Egypt.
  • Eman Ali Abdelrazik Lecturer of Forensic Medicine and Clinical Toxicology, Faculty of Medicine Mansoura Univerasity, Egypt.
  • Zainb Abdallah Mahmoud Lecturer of Physiology, Faculty of Medicine Mansoura University, Egypt.
  • Hend Mohammed Mohammed Hassan Lecturer of Anatomy and Embryology, Faculty of Medicine Mansoura University, Egypt
Keywords: Cardiomyopathy, Bisphenol A, Vitamin E, oxidative stress, autophagy, LC3, P62

Abstract

Introduction: Vitamin E (Vit E) is well known antioxidant. Bisphenol A (BPA), widely used industrial chemical product, is associated with increased risk for cardiac diseases to identify the potential protective effect of Vit E on BPA induced cardiomyopathy by alleviation of oxidative and autophagic stress through its antioxidant effect. Materials and Methods: Twenty –four adult male rates were used in the study. They were randomly divided into 4 groups; negative control, vit E positive control, BPA, and vit E treated group. All chemicals were given orally via gastric gavage for 14 days. Rats were sacrificed and their hearts were dissected out. Serum, cardiac homogenates, and cardiac tissues were obtained for biochemical and histopathological evaluation. Results: There were significant increase in serum DH and CK-MB, tissue homogenates showed elevated levels of NO and MDA and decreased level of GSH in BPA group. Immunohistopathological evaluation of autophagic mediators showed significant increase in LC3 and P62 in BPA group. On Histological examination, there was pathological alteration in BPA group compared to normal group. Vit E administration showed significant improvement in cardiac enzymes and oxidative stress. Also, alleviation of autophagic process and restoration of the myocardial architecture with reduction of the fibrous tissue were observed with vit E administration. Conclusion: These results demonstrate that vitamin E exhibit substantial protective effects in BPA induced cardiotoxicity by attenuating inflammation, oxidative stress, and alleviation the autophagic process.

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Published
2021-11-06
Section
Articles