Effects of Hyperbaric Oxygen Therapy on MMP-8 Saliva Levels in Chronic Periodontis: A Preliminary Research

  • Nirmala Putri Soranta Periodontist Resident, Faculty of Dentistry Padjadjaran University, Bandung, Indonesia
  • Ina Hendiani Department of Periodontics, Faculty of Dentistry, Padjadjaran University, Bandung, Indonesia cInstitute of RE. Martadinata Dentistry, Central Jakarta, Indonesia
  • Nunung Rusminah Institute of RE. Martadinata Dentistry, Central Jakarta, Indonesia
  • Ganesha Wandawa Hyperbaric Oxygen Chamber of dr. Mintrohardjo Naval Hospital, Central Jakarta, Indonesia
  • Suhadi
Keywords: Chronic periodontitis, hyperbaric oxygen therapy, MMP-8 levels


Introduction: Chronic periodontitis is inflammation of the periodontal tissue due to the accumulation of bacteria in plaque which causes progressive attachment loss and bone loss. Scaling root planing (SRP) is the initial treatment for periodontitis and often required as an adjunctive therapy to enhance the improvement of the inflammatory response. This study aimed to determine the effect of hyperbaric oxygen therapy (HBOT) on MMP-8 saliva levels in chronic periodontitis. Materials and Methods: This was an experimental research, non-randomized controlled trial with convenience sampling technique. The sample consisted of 9 patients with chronic periodontitis who were divided into 3 treatment groups, namely group 1 (SRP), group 2 (HBOT), group 3 (SRP+HBOT), each group consisted of 3 people. Measurement of MMP-8 levels in saliva was carried out before and after treatment on day 14. Data analysis to compare the difference in the mean levels of MMP-8 in the three treatment groups (before and after) was by using analysis of variance (F test); while to compare the decrease, the Kruskal-Wallis test was used. Comparison of MMP-8 levels between before and after treatment was by using paired t-test. Results: The difference in MMP-8 levels from the data before and after treatment in the combination of the three groups with analysis of variance (F test) did not show any significant difference (p>0.05); however, the difference in MMP-8 levels between before and after treatment in each group showed a significant difference (p<0.05) based on the Kruskal Wallis test; except in group 2 (HBOT) there was no significant difference (p>0.05). Conclusion: Administration of hyperbaric oxygen therapy (HBOT) after scaling root planing in chronic periodontitis patients had an effect on decreasing salivary MMP-8 levels.


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