Effects of Hyperbaric Oxygen Therapy on MMP-8 Saliva Levels in Chronic Periodontis: A Preliminary Research

  • Nirmala Putri Soranta Periodontist Resident, Faculty of Dentistry Padjadjaran University, Bandung, Indonesia
  • Ina Hendiani Department of Periodontics, Faculty of Dentistry, Padjadjaran University, Bandung, Indonesia cInstitute of RE. Martadinata Dentistry, Central Jakarta, Indonesia
  • Nunung Rusminah Institute of RE. Martadinata Dentistry, Central Jakarta, Indonesia
  • Ganesha Wandawa Hyperbaric Oxygen Chamber of dr. Mintrohardjo Naval Hospital, Central Jakarta, Indonesia
  • Suhadi
Keywords: Chronic periodontitis, hyperbaric oxygen therapy, MMP-8 levels

Abstract

Introduction: Chronic periodontitis is inflammation of the periodontal tissue due to the accumulation of bacteria in plaque which causes progressive attachment loss and bone loss. Scaling root planing (SRP) is the initial treatment for periodontitis and often required as an adjunctive therapy to enhance the improvement of the inflammatory response. This study aimed to determine the effect of hyperbaric oxygen therapy (HBOT) on MMP-8 saliva levels in chronic periodontitis. Materials and Methods: This was an experimental research, non-randomized controlled trial with convenience sampling technique. The sample consisted of 9 patients with chronic periodontitis who were divided into 3 treatment groups, namely group 1 (SRP), group 2 (HBOT), group 3 (SRP+HBOT), each group consisted of 3 people. Measurement of MMP-8 levels in saliva was carried out before and after treatment on day 14. Data analysis to compare the difference in the mean levels of MMP-8 in the three treatment groups (before and after) was by using analysis of variance (F test); while to compare the decrease, the Kruskal-Wallis test was used. Comparison of MMP-8 levels between before and after treatment was by using paired t-test. Results: The difference in MMP-8 levels from the data before and after treatment in the combination of the three groups with analysis of variance (F test) did not show any significant difference (p>0.05); however, the difference in MMP-8 levels between before and after treatment in each group showed a significant difference (p<0.05) based on the Kruskal Wallis test; except in group 2 (HBOT) there was no significant difference (p>0.05). Conclusion: Administration of hyperbaric oxygen therapy (HBOT) after scaling root planing in chronic periodontitis patients had an effect on decreasing salivary MMP-8 levels.

References

I. K. E. Wijaksana, “Periodontal Chart Dan Periodontal Risk Assessment Sebagai Bahan Evaluasi Dan Edukasi Pasien Dengan Penyakit Periodontal,” J. Kesehat. Gigi, vol. 6, no. 1, p. 19, 2019.

O.-C. J. Tonetti MS, Jepsen S, Jin L, “Impact of the Global Burden of Periodontal Diseases on Health, Nutrition and Wellbeing of Mankind: a Call for Global Action Maurizio,” J Clin Periodontol, vol. 38, no. 1, pp. 42–49, 2017.

K. Riskesdas, “Hasil Utama Riset Kesehata Dasar (RISKESDAS),” J. Phys. A Math. Theor., vol. 44, no. 8, pp. 1–200, 2018.

M. G. Newman, H. H. Takei, P. R. Klokkevold, and F. A. Carranza, Carranza’s Clinical Periodontology 11th Ed, vol. XXXIII, no. 2. 2012.

T. Yucel-Lindberg and T. Båge, “Inflammatory mediators in the pathogenesis of periodontitis.,” Expert Rev. Mol. Med., vol. 15, no. August, p. e7, 2013.

R. A. Kayal, “The role of osteoimmunology in periodontal disease,” Biomed Res. Int., vol. 2013, 2013.

E. F. de Morais, J. C. Pinheiro, R. B. Leite, P. P. A. Santos, C. A. G. Barboza, and R. A. Freitas, “Matrix metalloproteinase-8 levels in periodontal disease patients: A systematic review,” J. Periodontal Res., vol. 53, no. 2, pp. 156–163, 2018.

N. Johnson et al., “Rapid assessment of salivary MMP-8 and periodontal disease using lateral flow immunoassay,” Oral Dis., 2016.

A. Al-Majid, S. Alassiri, N. Rathnayake, T. Tervahartiala, D. R. Gieselmann, and T. Sorsa, “Matrix metalloproteinase-8 as an inflammatory and prevention biomarker in periodontal and peri-implant diseases,” Int. J. Dent., vol. 2018, 2018.

R. Rashid, “Hyperbaric Oxygen as a Therapeutic Approach in Periodontitis,” J. Adv. Med. Dent. Sci. Res., vol. 5, no. 10, pp. 369–373, 2017.

M. G. Newman, H. H. Takei, and P. R. Klokkevold, Carranza’s Clinical Periodontology, 12th ed. St. Louis, Missouri: Elsevier, 2015.

E. I. Wandawa, Ganesha; Mustaqimah, Dewi Nurul; Sidik, Suyanto; Saraswati, Henny; Putri, Frisky Amanda; Auekari, “Efficacy of Hyperbaric Oxygen Therapy as an Adjunctive Therapy of Chronic Periodontitis,” J. Int. Dent. Med. Res., vol. 10, no. 1, pp. 72–75, 2017.

D. Deepa and N. Jain, “Applications of hyperbaric oxygen therapy in dentistry: A mini review,” J. Interdiscip. Dent., vol. 4, no. 1, p. 27, 2014.

L. K. Weaver, [Hyperbaric oxygen therapy (indications and uses)]., vol. 61, no. 2. 2003.

C. Signoretto, F. Bianchi, G. Burlacchini, and P. Canepari, “Microbiological evaluation of the effects of hyperbaric oxygen on periodontal disease,” New Microbiol., vol. 30, no. 4, pp. 431–437, 2007.

L. Zhang, X. Li, H. Yan, and L. Huang, “Salivary matrix metalloproteinase (MMP)-8 as a biomarker for periodontitis,” Med. (United States), vol. 97, no. 3, pp. 1–6, 2018.

G. M. Nardi et al., “Evaluation of salivary matrix metalloproteinase (MMP-8) in periodontal patients undergoing non-surgical periodontal therapy and mouthwash based on ozonated olive oil: A randomized clinical trial,” Int. J. Environ. Res. Public Health, vol. 17, no. 18, pp. 1–10, 2020.

A. M. Marcaccini et al., “Circulating matrix metalloproteinase-8 (MMP-8) and MMP-9 are increased in chronic periodontal disease and decrease after non-surgical periodontal therapy,” Clin. Chim. Acta, vol. 409, no. 1–2, pp. 117–122, 2009.

Konopka, A. Pietrzak, and E. Brzezińska-Błaszczyk, “Effect of scaling and root planing on interleukin-1β, interleukin-8 and MMP-8 levels in gingival crevicular fluid from chronic periodontitis patients,” J. Periodontal Res., vol. 47, no. 6, pp. 681–688, 2012.

Q. Zhang and L. J. Gould, “Hyperbaric oxygen reduces matrix metalloproteinases in ischemic wounds through a redox-dependent mechanism,” J. Invest. Dermatol., vol. 134, no. 1, pp. 237–246, 2014.

N. Gupta, N. D. Gupta, A. Gupta, S. Khan, and N. Bansal, “Role of salivary matrix metalloproteinase-8 (MMP-8) in chronic periodontitis diagnosis,” Front. Med., vol. 9, no. 1, pp. 72–76, 2015.

V. Rangbulla, A. Nirola, M. Gupta, P. Batra, and M. Gupta, “Salivary IgA, Interleukin-1β and MMP-8 as Salivary Biomarkers in Chronic Periodontitis Patients,” Chin. J. Dent. Res., vol. 20, no. 1, pp. 43–51, 2017.

T. Chen, Y. Zhou, J. Liu, B. Xu, Z. Wu, and D. Li, “Biological effects of hyperbaric oxygen on human severe periodontitis,” Undersea Hyperb. Med., vol. 29, no. 3, pp. 159–166, 2002.

Published
2021-10-27
Section
Articles