Detection and Characterization of Cucumber mosaic virus Infecting Ginger (Zingiber officinale Roscoe) in Malaysia
Keywords:Ginger, Cucumber mosaic virus, Detection, Virus, Zingiberaceae, RT-PCR
Ginger (Zingiber officinale Rosc.) is herbaceous crop belonging to the family Zingiberaceae and cultivated for its medicinal properties and as marketable spice. A study was carried out to investigate Cucumber mosaic virus (CMV) infection in Malaysian ginger plants, showing conspicuous symptoms of mosaic, stripping and yellowing. A total of 45 symptomatic and 15 non symptomatic ginger samples were collected from three States in Malaysia. Reverse-transcription polymerase chain reaction (RT-PCR) analysis using CMV specific primers showed 14 out of 60 samples were positive for CMV. Cloning and sequence analyses of the 500 bp amplicons revealed that the CMV isolates obtained were closely related to CMV isolates from China (tomato) and Thailand (cucumber) with 95% to 96% sequence similarity respectively. Phylogenetic analysis placed the Malaysian CMV isolates into Subgroup IB. This is the first report of CMV infecting ginger in Malaysia
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