Anti-endometriotic Effects of the Mixture of Erigeron floribundus (Asteraceae) and Tragia benthamii (Euphorbiaceae) Extracts in Peritoneal Endometriosis in Wistar Rats
Keywords:
Endometriosis, Erigeron floribundus, Tragia benthamii, estrus cycle, sexual hormones, ratAbstract
Endometriosis is a frequent pathology in gynecology. This study was aimed at investigating the curative effects of the mixture of Erigeron floribundus and Tragia benthamii aqueous extracts on experimental endometriosis in rat. Twenty endometriosic rats were randomly distributed into four groups of five animals each and orally treated during four weeks with either distilled water (10 ml/kg), raloxifene hydrochloride (10 mg/kg) or aqueous extract of plant mixture (130 or 260 mg/kg). Vaginal smear was daily checked throughout the experiments and, blood and sexual organs were collected after sacrifice. Body and sexual organ weights, sexual hormones, prostaglandin E2, oxidative stress markers and uterine histology were measured. Results showed a disrupted estrus cycle, an increase in MDA concentration, implant weight and implantation surface in the untreated endometriosic rats. On the contrary, in the plant extracts-treated endometriosic animals and raloxifene, the estrus stage frequency was increased while the endometriosis lesions were significantly (p<0.001) regressed. Plant extracts also decreased the level of estradiol, progesterone, MDA and catalase, and increased the activities of SOD and peroxidase compared to the untreated rats.
Prostaglandin E2 concentration and the uterine architecture remained statistically unchanged after treatments. In conclusion, the aqueous extract of the mixture of E. floribundus and T. benthamii alleviates the endometriosic alterations in rats.
References
. G. Umit, C. Sabri, K. Sadiman, L. Omer, T. Anil, E. Ozlem. "Effect of the non-specific matrix metalloproteinase inhibitor Doxycycline on endometriotic implants in an experimental rat model". Experimental and therapeutic medicine, vol. 9, pp. 1813-1818, 2015.
. A. Suardika, T.G. Pemayun. "New insights on the pathogenesis of endometriosis and novel non-surgical therapies". Journal of the Turkish-German Gynecological Association, vol. 19, pp. 158-164, 2018.
. Y. Jin, Z. Li, Z. Danying, Dongxia Z., S. Wei, B. Lingling, et al. "The effects and possible mechanisms of Puerarin to treat endometriosis model rats". Evidence-Based Complementary and Alternative Medicine, vol. 2015, pp. 11, 2015.
. A. Fassbender, R. O. Burney, F. O. Dorien, T. D’Hooghe, L. Giudice. "Update on biomarkers for the detection of endometriosis". BioMed Research International, vol. 2015, pp.14, 2015.
. J.S. Tamaresis, J.C. Irwin, L.C. Giudice. "Molecular classification of endometriosis and disease stage using high-dimensional genomic data". Endocrinology, vol. 155, no 12, pp. 4986-4999, 2014.
. B. Borghese, D. Vaiman, X. D. Vaiman, C. Chapron. "Endometriosis and genetics: what responsibility for the genes?". Journal de Gynécologie Obstétrique et Biologie de la Reproduction, vol. 39, no. 3, pp. 196-207, 2010.
. C. Neukomm, M.D. Mueller. "Endometriosis – Alittle known often unrecognized disease ". Forum Medical Suisse, vol. 8, no. 30-31, pp. 539-543, 2008.
. E. Rolla. "Endometriosis: advances and controversies in classification, pathogenesis, diagnosis and treatment". F1000Research, Vol. 8, pp. 529, 2019.
. M. Wu, C. Lu, P. Chuang, S. Tsai. "Prostaglandin E2: the master of endometriosis". Experimental Biology and Medicine, vol. 235, pp. 668-677, 2010.
. S. Gennaro, I. Piergiorgio, B. Giulia, B. Gloria, S. Savino, A.V. Carlo, et al. "Oxidative stress and endometriosis: a systematic review of the literature". Oxidative Medecine and Cellular Longevity, vol. 2017, pp. 1-7, 2017.
. C. Azoulay. "Endométriose: options thérapeutiques médicales". Médecine thérapeutique/ médecine de la reproduction, vol. 9, no.1, pp. 41-5, 2007.
. Z. Yao, X. Shen, I. Capodanno, M. Donnelly, J. Fenyk-Melody, J. Hausamann, et al. "Validation of rat endometriosis model by using raloxifene as a positive control for the evaluation of novel SERM compounds". Journal of investigative surgery, vol. 18, no. 4, pp.177-83, 2005.
. S. Derouich, L. Attia, O. Slimani, A. Bouzid, N. Mathlouthi, R.B. Temim, et al. "Medical treatment of endometriosis ". La Tunisie médicale, vol. 93, no. 7, pp. 407- 412, 2015.
. M. Ilhan, Z. Ali, I.A. Khan, H. Tastan, E.K. Akkol. “ Bioactivity- guided isolation of flavonoids from Urtica dioica L. and their effect on endometriosis rat model”. Journal of Ethnopharmacology, vol. 243, pp. 112100, Oct 2019.
. F. Bina, M.Daglia, C. Santarcangelo, M. Baeeri, M. Abdollahi, S.M. Nabavi et al. “Phytochemical profiling and ameliorative effects of Achillea cretica L. on rat model of endometriosis”. Journal of Ethnopharmacology, vol. 254, pp.112747, May 2020.
. M.S. Essono, M.A. Mvondo, E. Ngadjui, K.F. Nguimatio, P. Watcho. "The ethanol extract of avocado (Persea americana Mill. (Lauraceae)) seeds successfully induces implant regression and restores ovarian dynamic in a rat model of endometriosis". Evidence-Based Complementary and Alternative Medicine, vol. 2020, pp.10, 2020.
. E.A. Asongalem, H.S. Foyet, J. Ngogang, G.N. Folefoc, T. Dimo, P. Kamtchouing. "Analgesic and anti-inflammatory activities of Erigeron floribundus". Journal of ethnopharmacology, vol. 91, no. 2-3, pp. 301-8, 2004.
. I.A. Oladosu, S.O. Balogun, G.O. Ademowo. "Phytochemical screening, antimalarial and histopathological studies of Allophylus africanus and Tragia benthamii". Chinese Journal of Natural Medicines, vol. 11, no. 4, pp. 0371-6, 2013.
. V. Kuete, L.P. Sandjo, A.T. Mbaveng, J.A. Seukep, B.T. Ngadjui, T. Efferth. "Cytotoxicity of selected Cameroonian medicinal plants and nuclea pobeguinii towards multi-factorial drug-resistant cancer cells". BMC Complementary and Alternative Medicine, vol. 15, pp. 309, 2015.
. B.S. Efferth, N.R. Rao, K. Vijeepallam, V. Pandy. "Phytochimical, Pharmacological and biological profiles of Tragia species (Family: Euphorbiaceae)". African Journal of traditional and complementary Alternative Medicine, vol.14, no. 3, pp.105-112, 2017.
. R. Petrelli, G. Orsomando, L. Sorci, F. Maggi, F. Ranjbarian, P.C. Nya, et al. "Biological activities of the essential oil from Erigeron floribundus". Molecules, vol. 21, pp.1065, 2016.
. European Parliament and the Council of the European Union. "Directive 2010/63/EU of the European parliament and of the council of 22 September 2010 on the protection of animals used for scientific purposes". Official Journal of the European Union, vol. 276, pp. 33-79, 2010.
. R.C. Jones. "The effect of a luteinizing hormone releasing hormone (LRH) agonist (Wy-40,972), levonorgestrel, danazol and ovariectomy on experimental endometriosis in the rat". Acta Endocrinology, vol. 106, no. 2, pp. 282-8, 1984.
. V.F. Amaral, E.A. Amaral, W. Amaral, L.C. Guarita-Souza, J.C. Francisco. "Development of an experimental model of endometriosis in rats". Revista do Colégio Brasileiro de Cirurgiões, vol. 36, no. 3, pp. 250-5, 2009.
. [25] F. Vivian, A. Eduardo, K. William, C. Luiz, C. Júlio. "Development of an experimental model of endometriosis in rats". Revista do Colégio Brasileiro de Cirurgiões, vol. 36, no. 3, pp. 250-5, 2009.
. [26] F.E. Pereira, P.R. Carvalho de Almeida, B.H. Dias, P.R. Leitao de Vasconcelos, S.B. Guimaraes, F. Medeiros. "Development of a subcutaneous endometriosis rat model". Acta Cirúrgica Brasileira, vol 30, no. 1, pp. 6-12, 2015.
. [27] H.P. Misra, I. Fridovich. "The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase". Journal of Biological Chemistry, vol. 14, no.10, pp. 3170-5, 1972.
. [28] U.A. Fridovich, L.I. Olsson, G. Carlin, A.C. bylund-Fellenius. "Inhibition of lipid peroxidation by spin labels: relationships between structure and function". Journal of Biological Chemistry, vol. 264, pp. 11131-11135, 1989.
. [29] T. Dimo, D.E. Tsala, J.D. Dzeufiet, B.V. Penlap, N. Njifutie. "Effets of Aliafia multiflora Stapf on lipid peroxidation and antioxidant enzyme status in carbon tetrachloride-treated rats". Pharmacologyonline, vol. 2, pp. 76-86, 2006.
. [30] P.V. Habbu, R.A. Shastry, K. M. Mahadevan, H. Joshi, S.K. Das. "Hepatoprotective and antioxidant effects of Argyreia speciosa in rats". African Journal Traditional Complementary and Alternative Medicines, vol. 5, no. 2, pp.158-164, 2008.
. [31] C. Cannet. "Artefacts- the difficulties of the histological technique, the gaggers of the fiixation ". Review France Histotechnology, vol. 17, pp. 11- 20, 2004.
. [32] Y.M. Khine, F. Taniguchi, T. Harada. "Clinical management of endometriosis-associated infertility". Reproductive Medicine and Biology, vol. 15, no. 4, pp. 217- 225, 2016.
. [33] R.L. Jones, S.A. Lang, J.A. Kendziorski, A.D. Greene, K.A. Burns. “Use of a mouse model of experimentally induced endometriosis to evaluate and compare the effects of Bisphenol A and Bisphenol AF exposure”. Environmental Health Perspectives, vol. 126, no. 12, pp. 127004-14, Dec 2018.
. [34] I.R. Cohen, M.L. Sims, M.R. Robbins, M.C. Lakshmanan, P.C. Francis, G.G. Long. "The reversible effects of raloxifene on luteinizing hormone levels and ovarian morphology in mice". Reproduction and Toxicology, vol. 14, no. 1, pp. 37- 44, 2000.
. A. R. Mustapha, E. K. Bawa, D. Ogwu, U. S. Abdullahi, A. A. Kaikabo. "Effects of ethanolic extract of Rhynchosia sublobata (Schumach ) Meikle on estrous cycle in Wistar rats". International Journal of Medicinal and Aromatic Plants, vol. 1, no. 2, pp. 122-7, 2011.
. A.M. Lemuel, B. Muhammad, L. Sodiq, I. Echoru, F. Ssempija, E. Owembabazi, et al. "Effect of cleome gynandra leaf extract on the estrous cycle and histology of the ovary and uterus of wistar albino rats". Anatomy Journal of Africa, vol. 8, no. 1, pp. 1385-1394, 2019.
. D. Altintas, A. Kocku, M. Tosun, M. Cetinkaya. "Comparison of the effects of raloxifene and anastrozole on experimental endometriosis". European Journal of obstetrics and Gynecology & Reproductive Biology, vol. 150 no. 2010, pp. 84-7, 2010.
. R. J. Caeiro, V.E. Cervino, M.L. Rentero, C. E. Crespo, A. O. Alvaro, M. Casillas. "Raloxifene: mechanism of action, effects on bone tissue, and applicability in clinical traumatology practice". The Open Orthopaedics Journal, vol. 2009, no. 3, pp. 14-21, 2009.
. S.W. Guo, D.L. Olive. "Two unsuccessful clinical trials on endometriosis and few lessons learned". Gynecologic and obsterict investigation, vol. 64, pp. 24-35, 2007.
. E. Chantalat, M. C. Valera, C. Vaysse, E. Noirrit, M. Rusidze, A. Weyl, et al. "Estrogen receptors and endometriosis". International Journal of Molecular Sciences, vol. 21, pp. 2815, 2020.
. G.J. Keutcha, S. Djiogue, D.S. Njimfo, C.F. Awoumfack, D. Njamen. "Evaluation of the estrogenic properties of aqueous extracts of Tragia benthamii Baker (Euphorbiaceae) and Graptophyllum pictum (Acanthaceae) and their ability to alleviate some menopausal symptoms induced by ovariectomy in Wistar rats". International Journal of Phytomedecine, vol. 8, no. 2016, pp. 366- 378, 2016.
. N. S. Sapronov, S. B. Kasakova. "Effects of synthetic and plant-derived selective modulators of estrogen receptors on depression-like behavior of female rats". Bulletin of Experimental Biology and Medicine, vol. 146, no.1, pp. 73-6, 2008.
. W. Nothnick, Z. Alali. "Recent advances in the understanding of endometriosis: the role of inflammatory mediators in disease pathogenesis and treatment". F1000Research 2016, vol.5, pp.186, 2016.
Downloads
Published
How to Cite
Issue
Section
License
Authors who submit papers with this journal agree to the following terms.