Sub-chronic Toxicity of Anti-ectoparasitic Formulation Comprising Cymbopogon Citrates Essential Oil and Jatropha Curcas Fixed Oil in Swiss Albino Mice

  • Biniam Endale Department of Anatomy, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia, PO box 2888
  • Mekbeb Afework Department of Anatomy, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia, PO box 2888
  • Asfaw Debella Traditional & modern medicine research directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia, PO box 1242
  • Wondwossen Ergetie Department of Pathology, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia, PO box
  • Solomon Mequanint Department of Pharmacology, School of Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia, PO box
Keywords: Cymbopogon citratus, anti-ectoparasite, medicinal plants, Sub-chronic toxicity, histopathology


Ectoparasites are found widely distributed in all agro-ecological zones of Ethiopia. The problems they cause can be alleviated through the use of traditional medicinal plants.  Cymbopogon citratus is one of the traditional herbs commonly used for such purpose.  This study was conducted to profile safety of the C. citratus based anti ectoparasitic formulation. The toxicity study had shown significant weight change in animal group that received 3.75% of the formulation. There were also significant changes in serum lipid profiles of treated group animals compared with the control. The level of uric acid in animals treated with 3.75% of the formulation showed significant increase compared with the control. Liver and kidney histopathology of both treatment group animals revealed few mononuclear leucocytic infiltrations.  The blood cell parameters and different biochemical findings had revealed no sign of toxicity. However; other measured parameters like the body weight, histological and some biochemical findings had shown both significant and insignificant sign of toxicities.  Therefore; it is important to consider the effect of the formulation in prolonged treatment on body weight, uric acid excretion and lipid metabolism.

Author Biography

Mekbeb Afework, Department of Anatomy, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia, PO box 2888
Associate professor of Anatomy


. B. Abegaz, G. Yohannes . “Constituents of the essential oil of Ethiopian Cymbopogon Citratus Stapf.” J Nat Product,vol., 46(3), pp 424-6, 1983.

. G. Addis. “Development of plant based medicines against major ectoparasites of ruminants.” Accomplishments and future directions proceeding of scientific review on the ongoing efficacy and safety investigations of traditionally used medicinal plants in Ethiopia, Nov. 15-17, 2013.

. J. Ademuyiwa, O. Seyifunmi, E. Elliot, Y. Komolafe, O. Olamide, A. Akintemi, et al. “Nephroprotective and Nephrotoxicity effects of ethanolic extract of Cymbopogon citratus (lemon grass) on wistar albino rats” World Journal of Pharmacy and Pharmaceutical Sciences, vol., 5(12), pp 244-256, 2013.

. J. Ademuyiwa, O. Seyifunmi, E. Elliot, Y. Ogunyemi, O. Olamide, O. Osabiya, et al. “ Nephroprotective effect of aqueous extract of Cymbopogon citratus(lemon grass) in Wistar albino rats.”.Int journal of Pharm and Bio Researches, pp 0341-0350, 2015.

. J. Ademuyiwa, Y. Ogunyemi, O. Olamide, O. Oyebiyi. “The Effects of Cymbopogon Citratus (Lemon grass) on the blood sugar level, lipid profiles and hormonal profiles of wistar albino rats.” American journal of toxicology, vol. 1, pp 8-18, 2014.

. J. Ademuyiwa, K. Oso. “The effects of Cymbopogon citratus (Lemon grass) on the anti oxidant profiles in wistar albino rats.” J Environ Sci Toxicology, vol., 3(4), pp 051-058, 2015.

. A. Adeneye, E. Agbaje. “Hypoglycemic and hypolipidemic effects of fresh leaf aqueous extract of Cymbopogon citratus Stapf in rats.” J Ethnopharmacology, vol., 112, pp 440–444, 2007.

. K. Aftab, M. Ali, P. Aijaz, N. Beena, H. Gulzar, K. Sheikh, et al. “ Determination of different trace and essential element in lemon grass samples by x-ray fluorescence spectroscopy technique.” Int Food Res J., vol., 18, pp 265-270, 2011.

. A. Akhila. Essential oil bearing plants: The genus Cymbopogon. CRC: Press Taylor Francis Group, 2010, pp 112-154.

. E. Arhoghro, C. Keh, T. Prohp. “Cymbopogon citratus aqueous extract alleviates cisplatin-induced hepatic oxidative stress and toxicity in albino rats.” Int journal of current microbiology and applied sciences, vol., 3(4), pp 586-604, 2014

. G. Berhanu, H. Hoekstra, J. Samson. “Heading towards commercialization.” Blackwell Scientific Publication, pp: 1-3, 2007

. T. Boudjeko, G. Ngomoyogoli, O. Woguia, Yanou. “Partial characterization, antioxidative properties and hypolipidemic effects of oilseed cake of Allan blackia floribunda and Jatropha curcas.” Complementary and Alternative Medicine, pp 313:352, 2013.

. J. Cheel, C. Theoduloz, J. Rodriguez, G. Schmeda. “Free radical scavengers and anti oxidants from Lemongrass (Cymbopogon citratus [DC.]Stapf).” J Agric Food Chem, vol., 53(7), pp2511-2517, 2005.

. Ethiopian Central Statistical Agency, “agricultural sample survey”. Internet: http:// , 2014/15, [Dec. 19, 2015].

. L. Davyson, A. Moreira, S. Sabrina, H. Maria, M. Monteiro, C. Ana, et al. “Traditional use and safety of herbal medicines.” Brazilian journal of pharmacognocy, vol. 24, pp 15-68, 2014.

. P. Fandohan, B. Gnonlonfin, A. Laleye, J. Gbenou, R. Darboux, M. Moudachirou. “Toxicity and gastric tolerance of essential oils from Cymbopogon citratus, Ocimumgratissimum and Ocimumbasilicum in Wistar rats.”.Food Chem Toxicol., vol., 46(7), pp 2493-2497, 2008.

. A. G/Mickael, G. Gebru, A. Debella, G. Addis, E. Mokonnen, Y. Bekuretsion. “The effect of C. citratus essential oil-water emulsion on some blood parameters and histopathology of Liver and Kidney in Mice.” International journal of Sciences: Basic and applied research, vol., 35, pp 182-192, 2017.

. K. Gamaniel.. “Toxicity from medicinal plants and their products.” Nigerian Journal of Natural Products and Medicines, vol., 4, pp 4-8, 2000.

. M. Guerra, J. Badell , A. Albajés, H. Pérez, R. Valencia, A. Azcuy. “Toxicologic acute evaluation of the fluid extracts 30 and 80 percent of Cymbopogon citratus (D.C.) Stapf (lemon grass).” Rev Cub Plantas Med.,vol., 5, pp 97-101, 2000.

. Y. Hailu. “Ectoparasitism: Threat to Ethiopian small ruminant population and tanning industry.” Journal of Veterinary Medicine and Animal Health, vol., 6, pp 25-33, 2014.

. Y. Hailu. “Skin defects in small ruminats and their nature and economic importance: The case of Ethiopia.” Global Veterinaria, vol., 11, pp12-23, 2013.

. International livestock research institution, “Livestock production and marketing in Ethiopia.” Internet: http//, 2015, [Feb. 13, 2016).

. K. Kassaye, Amberbir, B. Getachew, Y. Mussema. “A historical overview of traditional medicine practices and policy in Ethiopia.” Ethiopian Journal of Health Development, vol., 20, pp 127-134, 2006.

. B. Kumsa, K. Beyecha, M. Geloye. “Ectoparasites of sheep in three agro-ecological zones in central Oromia, Ethiopia.” Journal of Veterinary Research, vol., 79(1): pp7, 2012a.

. J. Leite, M. Seabra, E. Maluf, K. Assolant, D. Suchecki, S. Tufik, et al. “Pharmacology of Lemongrass (CymbopogoncitratusStapf).” J Ethno pharmacology, vol., 17 (1), pp 75-83, 1986.

. The Organization of Economic Co-operation and Development. “Guideline for testing of chemical: 420 Acute Oral Toxicity.” France. pp 1-14, 2001.

. A. Ogbe, G. George. “Nutritional and Anti nutrient Composition of Melon Husks: Potential as Feed Ingredient in Poultry Diet.” Journal of Chemical Sciences, vol., 2(2), pp 35-39, 2012.

. H. Omer, M. Elallawy, L. Abdel-Samee, N. Maghraby. “Productive performance of rabbits fed on diets containing lemongrass or active dried yeast.” American Eurasian J Agric & Envion Science, vol., 7(2), pp 179-187, 2010.

. A. Sommer. “Fresh lemon grass fields in Israel become mecca for cancer patients.” Internet: http//www., 2006, [Jan 12, 2015].

. P. Tarkang, G. Agbor, N. Tsabang, R. Tchokouaha, D. Tchamgoue, D. Kemeta, et al. “Effect of long-term oral administration of the aqueous and ethanol leaf extract of Cymbopogon citratus(DC. Ex Ness) Stapf.” Ann Biol Res, vol., 3(12), pp 5561-5570, 2012.