Assessment the Effect of Some Reagents on the Planktonic Cells and Biofilms of Red Complex Periodontal Pathogens

Abstract

The current study aimed to investigate the effectiveness of four regents; two naturals, olibanum and alum, and two standards, ciprofloxacin (CIP) and chlorhexidine (CHX) to affect the growth and biofilm of three types of periodontal pathogens, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, "the red complex group". Clinical isolates of the red complex pathogens were isolated from chronic periodontitis. They were identified by phenotypic properties and molecular method. The inhibitory activity of the four reagents was tested by microdilution method. Minimal inhibitory concentration (MIC) on the bacterial plankton and minimal biofilm inhibitory concentration (MBIC) on biofilm of the four reagents in a single and combinational use was determined on mono- and polymicrobial populations. Simple linear regression modeling was used to explore the effect of each reagent and determine MICs and MBICs. All reagents showed inhibition activity against the growth of mono- and polymicrobial planktonic population. MIC values on polymicrobial growth were higher than on monomicrobial growth and MBICs were much higher. All reagents had antibacterial activity on a monomicrobial biofilm with greater significant effect on T. denticola then T. forsythia and P. gingivalis. On polymicrobial biofilm, just olibanum continued showing its effect whilst CHX was less effect and both alum and CIP had no effect. Combinational use with Olibanum encouraged the effect of other regents on polymicrobial biofilm. This combination is a promising medicated preparation to combat the subgingival plaque of red complex pathogens.