The Effect of Vitamin C Giving on the Degree of Leucocyte Infiltration and Edema Alveolar in Increasing the Level of Arterial Oxygen in White Rats (Rattus Norvegicus) Having Pulmonary Contusion
Keywords:
lung contusion, leucocyte infiltration, alveolar oedema, PaO2.Abstract
Lung contusion is the most common cases diagnosed at intrathoracic injury resulting from blunt trauma. Lung contusion usually caused excessive reactive oxygen species production, a condition that will trigger lipid peroxidase that will cause damage to the cell membrane and cell lysis. Vitamin C as an antioxidant will act as a ROS scavenger to prevent lipid peroxidase. The aim of this research is to examine the effectiveness of vitamin C to increase the level of arterial blood oxygen and explain its pathomechanism. The research used experimental method conducted in the white rats (Rattus norvegicus). Study groups were divided into three groups, i.e.control group (CG, n=9), Lung contusion (LC, n=9), and pulmonary contusion given vitamin C (VC, n=9). For the experimental group, thoracic blunt trauma model was made by dropping the cylindrical iron load with the weight of (0.25 kg) on stainless steel pipe of the right hemithorax from the height of 80 cm (E = 1.96 J). For the VC group, the vitamin C of 200 mg/kg was injected 15 minutes after the intraperitoneal trauma. For 24 hours after the treatment, the examination of the blood gas analysis and pulmonary biopsy was conducted to find out oxygenation status and histopathological examination.
The result of the analysis indicates that the pulmonary contusion given vitamin C has better arterial PaO2 value than the pulmonary group without being given vitamin C (p = 0,019). This is caused by the decrease of leucocyte infiltration degree (p = 0.011) and alveolar oedema (p = 0,050). Vitamin C has influence as an antioxidant towards changes in leukocyte infiltration rate, alveolar edema, and blood artery oxygen level at Sprague Dawley white rat (Rattus norvegicus) suffered from lung contusion.
References
Raghavendran K, Davidson BA, Woytash JA, Helinski JD, Marschke CJ, Manderscheid PA, et al. 2005. The Evolution of Isolated Bilateral Lung Contusion From Blunt Chest Trauma in Rats: Cellular And Cytokine Responses. Shock. 24(2):132–8. PMID: 16044083
Bhatia M, Zemans RL, Jeyaseelan S. (2012). ‘Role of chemokines in the pathogenesis of acute lung injury’. Am J Respir Cell Mol Biol. 46 (5):566–72. doi: 10.1165/rcmb.2011-0392TR.
Rana S, Zeynep G, Mehmet S, Okan S, Bengu S, Yetkin A, Namuk D, 2013 Vitamin C as an Antioxidant: Evaluation of Its Role on Pulmonary Contusion Experimental Model. Turk J Med Sci. 44(6):905-13. doi:10.3906/sag-1309-139
Farley KS, Wang LF, Razavi HM, Law C, Rohan M, McCormack DG, et al. 2006. ‘Effects of macrophage inducible nitric oxide synthase in murine septic lung injury’. Am J Physiol Lung Cell Mol Physiol. 290 (6):1164–72. doi:10.1152/ajplung.00248.2005
Sies H. 1991. Oxidative stress: from basic research to clinical application. Am J Med. 91(3C):31S – 38S. PMID: 1928209
Ganie FA, Lone H, Lone GN, Wani ML, Singh S, Dar AM. 2013. ‘Lung contusion: A Clinico-Pathological Entity with Unpredictable Clinical Coourse’. Bull Emerg Trauma 1(1):7-6. PMID: 27162815 PMCID: PMC4771236
Boybeyi O, Bakar B, Aslan MK, Atasoy P, Kisa U, Soyer T. Evaluation of dimethyl sulfoxide and dexamethasone on pulmonary contusion in experimental blunt thoracic trauma. Thorac Cardiovasc Surg. 2014; 62(8): 710-5. doi: 10.1055/s-0033-1352591.
Raghavendran K, Notter RH, Davidson BA, Helinski JD, Kunkel SL, Knight PR. 2009. Lung Contusion: Inflammatory Mechanisms and Interaction with Other Injuries.shock. 32(2):122-30. doi: 10.1097/SHK.0b013e31819c385c
Türüt H, Ciralik H, Kilinc M, Ozbag D, Imrek SS. 2009. Effects of early administration of dexamethasone, N-acetylcysteine and aprotinin on inflammatory and oxidant-antioxidant status after lung contusion in rats. Injury. 40(5):521-7. doi: 10.1016/j.injury.2008.05.001
Cristante a F, Barros Filho TEP, Oliveira RP, Marcon RM, Rocha ID, Hanania FR,. 2009. ‘Antioxidative therapy in contusion spinal cord injury’. Spinal cord Off J Int Med Soc Paraplegia. 47 (6):458-63. doi: 10.1038/sc.2008.155.
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