Isolation and Characterization of Yeast Strains from Burdur Sugar Factory
Keywords:
Sugar factory, yeast, heavy metal resistance, azo dye removalAbstract
This study aimed to isolate and characterize yeast strains from diverse sampling points within the Burdur Sugar Factory, including pulp, bagasse, transport water, molasses, and filter press soil. Yeast isolation was performed using Rose Bengal Chloramphenicol Agar, and colonies were analyzed for growth at elevated temperatures, heavy metal resistance, killer toxin production, and textile dye removal capabilities. A total of 16 yeast strains were isolated, with the highest colony counts observed in pulp and bagasse samples, while no growth occurred in molasses or filter press soil. Physiological characterization revealed that 11 strains exhibited growth at 37 °C, and all strains demonstrated lead resistance at 5mM concentration, though resistance to other heavy metals was negligible. Killer toxin assays identified that BS12 effectively inhibited five other strains, highlighting its potential for fermentation applications. In the textile dye removal test, all strains grew in dye-enriched media, but only one strain BS8, isolated from bagasse, showed significant color removal. These findings suggest that the unique microenvironment of the sugar factory supports yeast strains with diverse and potentially valuable metabolic properties. Further molecular characterization is recommended to confirm species-level identification and explore the industrial applications of these yeasts in biotechnology, biofuel production, and environmental remediation.
References
G. A. M. Soares and H. H. Sato. “Killer toxin of Saccaharomyces cerevisae Y500-4L active against fleischmann and itaiquara commercial brands of yeast.” Revista de Microbiologia, vol. 30, pp. 253-257 ISSN 0001-3714, 1999.
Z. A. Ibrahim, S. H. Khudheir, A. A. Hussein. “Isolation, screening, and extraction the more efficient local yeast isolates for biosurfactant production.” Earth Environ. Sci., vol. 779, pp. 1-8, 2021.
L. He, Y. Chen, H. Zhang, H. Wang, S. Chen, S. Liu, A. Liu, Q. Li, X. Ao, Y. Liu. “Isolation and identification of Lactobacillus and yeast species and their effect on the quality of fermented rice cakes.” Innovative Food Science & Emerging Technologies, vol. 77, pp. 1-10, 2022. https://doi.org/10.1016/j.ifset.2022.102984.
W. Irawat, Y. Wijaya, S. Christian, E. S. Djojo. “Characterization of heavy metals resistant yeast isolated from activated sludge in Rungkut, Surabaya, Indonesia as biosorbent of mercury, copper, and lead.” AIP Conf. Proc., 1744, 020061, 2016. https://doi.org/10.1063/1.4953535
R. Jamee and R. Siddique. “Biodegradation of synthetic dyes of textile effluent by microorganisms: an environmentally and economically sustainable approach.” European Journal of Microbiology & Immunology, vol 9(4), pp. 114–118, 2019. https://doi.org/10.1556/1886.2019.00018
M. Tripathi, S. Singh, S. Pathak, J. Kasaudhan, A. Mishra, S. Bala, D. Garg, R. Singh, P. Singh, P. K. Singh, A. K. Shukla, N. Pathak. “Recent Strategies for the Remediation of Textile Dyes from Wastewater: A Systematic Review. Toxics, 11(11), 940. 2023 https://doi.org/10.3390/toxics11110940
R. Khan, P. Bhawana, M. H. Fulekar. “Microbial decolorization and degradation of synthetic dyes: A review.” Rev Environ Sci Bio/Technol, vol. 12, pp. 75–97, 2013.
R. G. Saratale, G. D. Saratale, J. S. Chang, S. P. Govindwar. “Bacterial decolorization and degradation of azo dyes: A review.” J Taiwan Inst Chem Eng., vol. 42(1), pp. 138–57, 2011.
N. Jafaria, M. R. Soudib, R. Kasra-Kermanshahi. “Biodegradation Perspectives of Azo Dyes by Yeasts.” Microbiology, pp. 484–97.25844460, 2014.
G. Guo, F. Tian, Y. Zhao, M. Tang, W. Liu, C. Liu, S. Xue, W. Kong, Y. Sun, S. Wang. “Aerobic decolorization and detoxification of acid scarlet GR by a newly isolated salt-tolerant yeast strain Galactomyces geotrichum GG.” Int. Biodeterior. Biodegrad., vol. 145, 104818, 2019. DOI:10.1016/j.ibiod.2019.104818
A. C. R. Ngo, D. Tischler. “Microbial degradation of azo dyes: Approaches and prospects for a hazard-free conversion by microorganisms.” Int. J. Environ. Res. Public Health., vol. 19, pp. 4740, 2022.
J. Jadhav, S.P. Govindwar. “Biotransformation of malachite green by Saccharomyces cerevisiae MTCC 463.” Yeast, vol. 23, pp. 315–323, 2016.
S. U. Jadhav, S. D. Kalme, S. P. Govindwar. “Biodegradation of methyl red by Galactomyces geotrichum MTCC 1360.” Int. Biodet. Biodeg., vol. 62, pp. 135–142, 2010.
A. Sadeghi, M.H. Ehrampoush, M.T. Ghaneian, A.A. Najafpoor, H. Fallahzadeh, Z. Bonyadi. “The effect of diazinon on the removal of carmoisine by Saccharomyces cerevisiae.” Desalination Water Treat., vol. 137, pp. 273–278, 2019.
F. Ruscasso, L. Cavello, G. Curutchet, G. “Antarctic yeasts: potential use in a biologic treatment of textile azo dyes.” Bioresour. Bioprocess., vol. 9 (18), pp. 1-12, 2022. https://doi.org/10.1186/s40643-022-00507-5
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