Isolation and Purification of Acetylcolinesterases (AchE ) from Blood of Thalassimic Patient and Kinetic Studies for Purified Enzyme

Authors

  • Nazar Ahmed Naji Department of Chemistry / College of Science / Tikrit University, Iraq
  • Sayran Sattar Saleh Department of Chemistry / College of Science / Kirkuk University, Iraq
  • Galawesh Noori Taher Department of Nursing/ Instituted Kirkuk Technical/ Northern Technical University, Iraq

Keywords:

Acetylcolinesterase, β- thalassemia, Purification, Dialaysis.

Abstract

In this research to isolate and study the properties of the Acetylcolinesterase  (Ec 3 .1.1.7) enzyme in the blood of a patient suffering from ?- thalassemia major and study kinetic  studies for purified  AchE .The study involves taking(5ml) of crude serum of thalassemic patients and subjected to a series of purification  processes including : precipitation by ammonium  sulfate , filtration by centrifugation radiator , dialaysis in presence of Tris-HCL,separation using the technology of gel and then Estimation approximate Molecular Weight of Partially Purified AchE using gel filtration technique and sodium dodecyl sulfate (SDS)-pag polyacrylamid gel electrophoresist.The result of the study indicates that AchE has an approximat molecular weight of 173720 Dalton.The kinetics of the enzyme were studied and the results showed that the maximum velocity was 7.8 µmol/min/mol and the Km was 0.1 M whil the optimum temperature of the enzyme was( 37  )and the optimum pH was( 7.4) .

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Published

2018-11-02

How to Cite

Naji, N. A., Saleh, S. S., & Taher, G. N. (2018). Isolation and Purification of Acetylcolinesterases (AchE ) from Blood of Thalassimic Patient and Kinetic Studies for Purified Enzyme. International Journal of Sciences: Basic and Applied Research (IJSBAR), 42(2), 252–264. Retrieved from https://gssrr.org/index.php/JournalOfBasicAndApplied/article/view/9353

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