Murine Models for Pathologenicity of Human Neurotropic Stealth and Normal Hemophilus Influenzae B [HIB]
Keywords:
Animal, cell wall defective, dose, infectious, intact, modelsAbstract
Human neurotropic stealth and normal intact Hemophilus influenzae b [HIB] were recovered from cases of subacute meningitis pateints.Tempts were made to reproduce the disease in murine models .Two infectious live doses as one and ten international units of the WHO opacity tube were prepared from both of the stealth and intact isolates.The dose volume was fixed as 0.1 ml.These infectious doses were applied to the murine models via intracranial and intravenous routes.The matching of gross neurogenic signs and symptomes of the infected mice were lasted up to ten days post-infection.At the dose 10 IU ,both stealth and intact HIB cause sudden death.While,1 IU doses from both stealth and intact HIB yield neurogenic symptomes from 7 to 10 days then the animals died. The 10 IU doses from both stealth and intact HIB via intravenous routes were leading to mild neurogenic symptome ranged from 2 to 24 hrs then vanished.While , 0.1 doses from both forms of the infectious agents showed symptomless state.The pathogenicity spectrum was as;symptomeless,mild neurogenic symptome ,sheffering,laid down on one body site on the stratum then death and/or sudden death.Reisolation was fruitfull in all infected animals. The direct impression stained smears of the infected mice brains showed landmarks of sub-acute meningitis.Intracranial mice model seems to be novel for simulation of human subacute meningits disease.
References
. ECDPC .Fact sheet about Hemophilus influenzae invasion .1-5, 2024.
. Levinson W ,Chin-Hong P,Joyce EA Nubssbaum J ,Schwartz ZB.Review of Medical Microbiology and Immunology.15th ed. NY USA, McGraw-Hill Lange, 2018,164-168.
. Caroll KC ,Hobden JA ,Millers S ,Morse SA ,Mitchell TG,McKerrow JH ,Sakanari JA.Jawetz ,Melnick and Adelberg's Medical Microbiology 27th ed NY,USA,.McGraw-Hill Lange,2016,263-265.
. Miyazaki S ,Matsumoto T ,Furuya N et al.The pathogenic role of fimbriae of Hemophilus influenzae b in murine bacteremia and meningitis.J.Med.Microbiol.48(4):383-388,1999.
. Marks MI , Ziegler EJ , Douglas BC Corbeil lB 1982.Lethal Hemophilus influenzae b in mice.Infection 10:261-266,1982.
. Dundo SJ et al.Pathogens penetrating the central nervous system:infection pathway and cellular and molecular mechanisms of invasion.Clin.Microbiol.Rev.47(4):691-726 ,2014.
. Wong SMS ,Akerley BJ.Genome scale approache to identify gene essential for Hemophilus influenzae pathogenesis.Cellular and Infection Microbiol.2;Article no.23.2012, doi.10.3389/fcmb.2012.00023.
. Banchello T , Ivoino F.Host-Pathogen interaction in central nervous system .Front .Cellular.Infect.Microbiol.11:790761.2021,doi.10.3389/fcimb.2021.790761.
. Casselli T , Divan A , Vomhof-DeKrey EE et al.A murine model for lyme disease Borrelia burjdorferi colonize the dura meter and induces inflammation in central nervous system.Plos.pathogen.17(2):e 1009256 2020,.doi.10.1371/journal.ppat.1000256.
. Hood D ,Moxon R Purnell T et al.A new model for nontypable Hemophilus influenzae in the Junbo mutatnt mouse.Dis,Model.and Mechanisms.9:69-79,2016.doi. 10. 12342/dmm.021659.
. Cheeseman MT , Hood DW..Nontypable Hemophilus influenzae infection of Junbo mouse.Curr.Protocols in Mouse Biology.Wiley Online liberary.,2017.
. Venuprasad K , Teivanthiran B ,Cantavel B .Intra-trachial admistraion of Hemophilus influenzae b in mouse model to study airway inflammation.J.Vis. Exp. 109:e53964. 2016,doi.10.3791.
. Virji M , Hill D .Animal Models.In Herbert Ma ,Hood DW Moxon E eds.Hemophilus influenzae Protocols.Methods And Molecular Mechanisms.V77,Humana Press,2003.
. Sulc P , Hengstler B , krink G et al.Experinmental rabbit model of meningitis produced by Hemophilus influenzae C.J.Med.Microbiol.312-317,1992,.doi.10.1099/022815-36-312.
. Cruickshnk R et al.Medical Microbiology.12th ed volume 2;The Practice of Medical Microbiolgy,UK,Churchill livingstone,1975,321-324.
. AL_Nasery Q N.Biology of Cell Wall Defective Microbes From Persistent Pyuria.Ph.D Thesis Department of Biology ,College of Science,University of Babylon,Babylon,Iraq,2003.
. Gonzalez MD, Ledeboer NA .Hemophilus,Manual of Clinical Microbiology,13th ed.In Patel R ed.. USA,ASM2023,pp.
. Ledeboer NA ,Doern GV.Hemophilus Chapter 36,Maual of Clinical MicrobiologyIn James and others.eds,USA,ASM, 2015,677-684.
. Chiavolini et al.Method for induction of experimental meningitis in outbreed mice.BMC .Medicine 4(3^):1-10,2004
. Divani AA et al..Coronavirus Diseae-19 and stroke:A clinical manifestation and pathophysiological insight.J.Stroke .Cerebrovascular Dis.29(8):104941,2020
. Koedel U , Pfister HW .Models For experimental bacterial meningitis.Role and limitations. Infect. Dis.North.Am.13(3):549-577,1999.
. Paul R et al.Using Knokout mice to study experimental meningitis. Arch.Immun. Ther.Exp.51:315-326.2003.
. Shnawa IMS,Thewaini QNA,Omran A H .S.aureus and Escherichia coli experimental induced septic and cryptic arthritis.Med. J Bay.6(1);20-27,2009.
. Thewaini QNA,Shnawa IMS,AL-Shibeeb AR,Mosa M.Lapin Citrobacter urogenital infection model.Ind.J.Pub.Health.Dev.11(2): 1919-1923,2020.
. Scheld W M , Bacterial meningitis in patients at risk and host defense mechanism. Am. J.Med.66:193-207.,1980.
. Moxon ER .Pathogenesis of meningitis,experimental studies on the molecular basis of Hemophilus influenzae b.Infection.12:523-527,1985.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 International Journal of Sciences: Basic and Applied Research (IJSBAR)

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who submit papers with this journal agree to the following terms.