analysis of TCR repertoire V? gene clonality in chronic hepatitis B patients

simeneh zelalem limenh (simenehze@gmail.com)
clinical laboratory, beihua univeristy
July, 2013
 

Abstract

Objective: To determine the T-cell receptor repertoire V? gene clone type in the peripheral blood of chronic hepatitis B patients
Method: Complementary DNA was synthesized from RNA which was extracted from peripheral blood mononuclear cells of chronic hepatitis B patients, amplified by RT-PCR. Clonality of each amplified product was observed by imaging through scanning by gel documentation and image analyzing system (champ gel 5550) after polyacry amide gel electrophoresis (PAGE).
Result: The rate of skewed(monoclonal/oligoclonal) TCR V? families was higher in CD8+ T-cell subsets(35/43.9%) than in CD4+ subsets(24/29.2)(p<.01). Monoclonal TCR V?9 and TCR V?14 have been found in a relatively high frequency in CD8+ T cells and TCR V?1 and TCR V?2 in CD4+ T-cells
Conclusion: Our research on the analysis of TCR repertoire V? gene may be useful in understanding and evaluation of the immune mechanism against chronic hepatitis B virus infection. It can also be helpful to design new immunotherapic strategies for chronic hepatitis B. The finding of a relatively high frequency existence in some TCR V? families (TCR V?9, TCR V?14 and others) in these patients could be used in monitoring the health status of the patient