New Synthesized Derivatives from N-Substituted-4-Oxo-[1] Benzopyrano [4,3-c] Pyrazole Influenced Proliferation, Viability, Spreading and Invasion of Human Liver Tumor Cells

Hela Guesmi, Oualid Haddad, Kevin Bassand, Rafik Gharbi, Moncef Msadek, Frederic Chaubet, Marc Lecouvey, Raoui Mounir Maaroufi, Hanna Hlawaty

Abstract


Background/Aim: There is an unsatisfied clinical demand to develop new anticancer agents. The aim of the current study was to synthesize new coumarin derivatives using two different synthetic methodologies and to evaluate their anticancer activity. Materials and methods: Four coumarin derivatives were synthesized and evaluated for their anticancer activities. The structures of all compounds were confirmed by infrared (IR), UV-vis, Nuclear magnetic resonance (NMR) 13C NMR, 1H NMR, and high-resolution mass spectrometry (HRMS) analysis.

All the synthesized compounds (4, 5, 8 and 9) were analyzed for their anti-proliferative (MTT and LDH assays and cell cycle studied with flow cytometry) and anti-invasive activity (spreading and invasion tests) on human hepatoma cell lines Huh-7 in vitro. Doxorubicin was used as control in order to compare their anti-tumoral effects. Results. All the synthesized compounds are potential inhibitors of proliferation, viability, spreading and invasion of human liver tumor cells with a 50% inhibitory Concentration range,  IC50=10.37 μM to 12.94 μM. Conclusion. This study could lead to the identification of a new target therapy for human Hepatocellular carcinoma (HCC) or other cancers.


Keywords


Fraud pentagon; fraudulent financial reporting; non-financial company.

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