The Correlation of BRCA 1 Promoter Methylation and Clinicopathological Appearance in Breast Cancer

Sony Sugiharto, Muh. Nasrum Massi, Syariffudin Wahid, Andi Fachruddin Benyamin, Nurjati Chaerani Siregar, Upik Anderiani Miskad, William Hamdani, Mochammad Hatta, Ilhamjaya Patellongi, Rosdiana Natsir


Breast cancer is one of the common cancers in the worldwide. Most of breast cancer are sporadic. BRCA1 expression levels are reduced or totally loss in sporadic breast cancer. BRCA1 promoter methylation as one of the mechanisms to inactivating its function. BRCA1 promoter methylation associated with triple negative breast cancer (TNBC), poor prognosis, high grade, negativity of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2(HER2). This study aims are to examine prevalence and correlation between BRCA1 promoter methylation and clinicopathology appearance in Indonesian women breast cancer. Subject are women with primary breast cancer and their formalin-fixed paraffin -embedded (FFPE) tumor specimen retrieved.

DNA was isolated and subjected to methylation specific PCR(MSPCR). DNA was isolated from primary tumor of 113 samples. Median age at diagnosis was 48 years (with range 28-80 years). Most of them, 67(59,3%) are include in aged categories <50 years. Incidence BRCA promoter methylation was found 82,3% (93 of 113.) There is significant correlation with BRCA1 promoter methylation with age<50 years old (p-value = 0.038) and Luminal B subtype (p-value = 0.033). Conclusion: BRCA1 promoter methylation in Indonesian women higher than other nations, correlate with younger patient and Luminal B subtype.


Breast cancer; BRCA1 promoter methylation; prognosis.

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