Isolation and Molecular Identification of Lactic Acid Bacteria (Lab) from Nile Tilapia (Oreochromis niloticus) as Potential Pathogen Antagonist

Jacqueline V. Bagunu, Evelyn V. Totaan, Christian R. Pangilinan

Abstract


Five lactic acid bacterial isolates from the intestinal tract of Nile Tilapia (Oreochromis niloticus) were tested for their antagonistic activity against common human pathogens. Only three were identified using 16S rRNA gene sequencing as Pediococcus pentosaceus (isolates TI-8 and TI-11) and Enterococcus avium (TI-17) showing 99% homology. Among the isolates, TI-8 and T1-11 (P. pentosaceus) viable cell cultures were found to have the highest inhibitory activity against Escherichia coli ATCC 25922, surpassing that of the positive control, Tetracyline. Though showing significant inhibition also to Pseudomonas aeruginosa BIOTECH 1335, Staphylococcus aureus BIOTECH 1582, Salmonella enteriditis BIOTECH 1963, Salmonella typhimurium BIOTECH 1826, and Vibrio cholerae BIOTECH 1967, T1-17 (Enterococcus avium) is not effective against S. aureus BIOTECH 1582. On the other hand, T1-8 and T1-11 (P. pentosaceus) cell free supernatant cultures exhibited inhibitory activity against P. aeruginosa BIOTECH 1335, S. aureus BIOTECH 1582, S. enteriditis BIOTECH 1963, S. typhimurium BIOTECH 1826, and V. cholerae BIOTECH 1967. 

The LAB isolate T1-17 (E. avium) exhibited  an  inhibitory  activity against S. enteriditis BIOTECH and V. cholerae  BIOTECH 1967. T1-11 (P. pentosaceus) was susceptible to Tetracycline and Amoxicillin; T1-8 (P. pentosaceus)  was susceptible to Amoxicillin and Cephalexin; and   T1-17 (E. avium) was susceptible to Amoxicillin.   


Keywords


antibacterial; human enteric pathogen; Lactic Acid Bacteria; Nile tilapia .

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