Bacterial Flora of Arachis Hypogea Plants from Agricultural Fields of Punjab, Pakistan

Uqba Mehmood, Muhammad Faisal

Abstract


The study was carried out to identify the rhizo-microorganisms from the roots of Arachis hypogea plants collected from agricultural fields of upper Punjab (Chakwal), Pakistan. Eight purified bacterial isolates were further identified by 16S rRNA gene sequencing. Bacillus spp were found to be most abundant in the rhizophere of Arachis hypogea. The isolated bacteria were mostly mesophilic (grow at 37°C and 42°C) and resistant to chloramphenicol. All isolates were found to be resistant to metals such as Mn, Pb, Co and Cr (100 µg/ml). However, these were sensitive to Hg and Cd at all the concentrations studied (100, 500 & 1000 µg/ml). Almost all isolates were sensitive to all metals at higher concentrations except few isolates which were resistant to Zn. The plant growth promoting ability was tested for Arachis hypogea seeds in pot experiment. No nodule formation was observed with any of the isolates while all of them promoted the growth of Arachis hypogea.


Keywords


Rhizosphere; Bacteria; Arachis hypogeal; Metal Resistance; Antibiotics; Acid Phosphatase.

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References


F. Buscot, What are soils? in Microorganisms in soils: roles in genesis and functions. Buscot F, Varma S, Ed. Germany:Springer-Verlag Heidelberg, 2005.

J.S. Singh, V.C. Pandey, D.P. Singh, “Efficient soil microorganisms: a new dimension for sustainable agriculture and environmental development”. Agriculture Ecosystems and Environment, vol.140, pp. 339-353, 2011.

J.M. Barea, R. Azco´n, C. Azco´n-Aguilar, Mycorrhizal fungi and plant growth promoting rhizobacteria. in Plant surface microbiology. A. Varma, L. Abbott, D. Werner, R. Hampp. eds. Heidelberg, Germany: Springer-Verlag, 2004.

M. Buée, W. De Boer, F. Martin, Overbeek IV, E. Jurkevitch, “The rhizosphere zoo: an overview of plant associated communities of microorganisms, including phages, bacteria, archaea, and dungi, and some of their structuring factors”. Plant Soil, vol. 321, pp. 189-212, 2009.

M. Gryndler. Interactions of arbuscular mycorrhizal fungi with other soil organisms. in Arbuscular mycorrhizas: physiology and function.Kapulnik Y, Douds Jr DD, eds. Dordrecht, the Netherlands: Kluwer Academic Publishers, 2000.

B. Yousuf, J. Keshri, A. Mishra, B. Jha. Application of targeted metagenomics to explore abundance and diversity of CO2- fixing bacterial community using cbbL gene from the rhizosphere of Arachis hypoge. Gene 2012. doi:10.1016/J.Gene.2012.06.083

P. Gerhadt, R. G. E. Murray, W. A. Wood, N. R. Krieg, Methods for general and molecular bacteriology. American Society Microbiology. 1325 Massachuestts Ave., NW Washington, DC, 1994.

K. C. G. Goes, D. Pinheiro, M. L. C. Fisher, A. J. Cattelan, M. A. Nogueria, C. G. P. Carvalho, A. L. M. Oliveria, “Biochemical and Molecular Characterization of high population density bacteria isolated from sunflower”. Journal of Microbiolgy and Biotechnology, vol. 22:4, pp. 437-447, 2012.

B. Ramos, J. M. Pozuelo, N.“Acero. Seasonal variations of Bacillus isolated from rhizosphere of Elaeagnus angustifolia. L Orsis. Vol. 13, pp. 7-16, 1998.

R. M. Atlas, R. Bartha, Microbial Ecology: fundamentals and applications. Menlo Park, CA: Benjamin/Cummings, 1993.

A. Probanza, J. A. Lucas, N. Acero, F. J. Gutiérrez, “The influence of native rhizobacteria on European alder [Alnus glutinosa (L.) Gaertn.] growth. I. Characterization of growth promoting and growth inhibiting bacterial strains”. Plant Soil, vol.182, pp. 57-62, 1996.

G. A. Strobel, D. M. Long. “Endophytic microbes embody pharmaceutical potential”l. ASM News, vol. 64, pp. 263-268, 1998.

S. Pleban, L. Chernin, I. Chet. “Chitinolytic activity of an endophytic strain of Bacillus cereus”. Letter of Applied Microbiology, vol.25, pp 284-288, 1997.

E. A. B. Emmert, J. Handlesman, “Biocontrol of plant disease: a (Gram-) positive perspective”. FEMS Microbiolgy Letters. Vol.171, pp. 1-9, 1999.


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