Inhibitory Efficacy of Geranium stepporum L. extracts Against Some Species of Bacteria and Fungi

Mostafa Qahtan Mosataf, Sirwan Hassan Salih, Talib Ouaid Alkhazraji

Abstract


Inhibitory efficacy of Geranium stepporum L.  extracts (aqueous, ethanol and methanol) against two gram positive pathogenic bacteria (Staphylococcus aureus, Streptococcus lactis), two gram negative (Escherichia coli and Proteus mirabilis) and four pathogenic fungi (Microsprum canis, Trichophyton mentagrophytes, Pencillium chrysogenum and Fusarium oxysporeum) was studied, tannins and flavonoids contents by using HPLC technique was identified as well glycosides and tannin extracted to testing its biological activities. The results showed that the examined plant contains all the active compounds that were detected, the glycoside with 15.64mm diameter was the most effective against the studied bacteria compared to the negative control and the other studied extracts, Proteus mirabilis with 11.06 diameter is the most affected by the extracts compared to the rest examined bacteria, likewise the results showed that the extracts of water (hot and cold), alcohol  (ethanol, methanol, and methanol) and glycosides were more effective against the  examined fungi than tannin extract, the species T. andagrophytes with 12.11mm and F. oxysporeum with 11.48mm diameter were most affected by all extracts compared to other tested fungi, quercetin, rutin, kaempferol, and tannins were found to have different concentrations. The quercetin at 1.45ppm and rutin at 1.23ppm. were the most concentrated than other studied compounds, the results also seemed a difference in the concentration of active compounds between the entire aerial plant parts and underground parts.


Keywords


phytochemistry; active compounds; abstracts; Geranium stepporum.

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References


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