The Impact of Diluted Detergents on Escherichia coli K12 (JM109)

Kahlan Abdullah Yahya Al-Beshari, Talal Abdullah, Anis Safirah Mohammad, Mohammed Razip Samian, Aida Baharuddin


The purpose of this study is to investigate the effect of diluted detergents namely, Clorox, Dettol and Aganol against Escherichia coli K12 (JM109), at different concentrations. Frequent usage of diluted detergents and disinfectants without knowing their effectiveness in killing microbes can act as a medium for infections in susceptible hosts. Moreover, it is known that the regular application of diluted detergents and disinfectants may actually cause antibiotic resistance. The efficacy of diluted detergents is determined by the minimum inhibition concentration (MIC) and the minimum bactericidal concentration (MBC) using tube dilution assay. The susceptibility test is conducted using the disc diffusion technique. Dettol and Clorox exhibited effective bacteriostatic agents where the MIC is 0.75%. The lowest concentration of Dettol and Clorox required to kill E. coli K12 (JM109) or MBC is at the concentration of 3.0% and 5.0%, respectively. Aganol showed less effective bacteriostatic and bactericidal agents, where it required higher MIC of 1.25% and MBC of 10%. The susceptibility test indicated that the Clorox is the most effective antibacterial agent with the minimum inhibition zone of 7 mm at a concentration of 1.75%. Higher concentrations of Dettol and Aganol (of 10% and 20%, respectively) are needed to exhibit the antibacterial activity with the minimum inhibition zone of 7 mm.


Clorox; Dettol; Aganol; E. coli K12 (JM109); minimum inhibition concentration (MIC); minimum bactericidal concentration (MBC).

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