Expression of Toll-like Receptor-4 (tlr-4) in Balb/c Mice Induced by Salmonella Typhi

Fadhilah Fadhilah, Gemini Alam, Mochammad Hatta, Rosdiana Natzir, Burhanuddin Bahar


Salmonella typhi is a gram negative bacteria that causes systemic infections and typhoid fever in humans. It has caused many deaths in developing countries.  WHO (World Health Organization) estimated the incidence rate worldwide approximately 17 million per year with 600,000 deaths  and 70% of them occurred in Asia (WHO, 2008). By Toll-like receptor  4 (TLR-4), macrophages can recognize lipopolysaccharides (LPS) present in S. typhi. The bond of LPS and TLR-4 that activates MyD88 plays an important role in controlling bacterial exponential growth. This study aimed to analyze the TLR-4 mRNA in Balb/c mice induced by S.typhi. This study was a laboratory experimental research on Balb/c mice with simple randomized design. Mice blood was taken before and after  induction by 103 CFU of S.typhi. Then the blood samples were examined at Microbiology Laboratory in Faculty of Medicine, Hasanuddin University to measure expression of Toll-like receptor-4 (TLR-4) mRNA using Real Time Polymerase Chain Reaction (RT-PCR). The result of this study showed that there were enhancement of TLR-4 expression from 20 samples obtained. The data indicated that S.typhi induction can increase TLR-4 expression.


Toll-like receptor-4 (TLR-4); Salmonella typhi; Real Time- Polymerase Chain Reaction (RT-PCR).

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