Production of Bacteriocin Like Substances as Antipathogenic Metabolites by Bacillus licheniformis Isolated from Healthy Human Skin

Reazul Karim, Nuruddin Mahmud, Md. Sharifuzzaman, Hasibul Islam

Abstract


Different types of bacterial colony were isolated from skin of 30 healthy human and their antipathogenic activity was tested against 9 pathogens. The isolate showed activity against pathogen- Kl. pneumoniae subsp. pneumoniae, was identified as Bacillus licheniformis Variation was found in optimization of cultural conditions (incubation period, incubation temperature and pH) for the most potent antipathogenic metabolites production. Bacillus licheniformis showed most potent antipathogenic activity at pH 7, at an incubation period of 48h and at an incubation temperature of 250C. Antipathogenic metabolites was then detected as bacteriocin like substances. Samples containing bacteriocin like substances were characterized with respect to their heat and pH stability and susceptibility to denaturation by the enzyme. It showed heat stability up to 800C for 30 minutes, stability up to pH 7. Papain treated cell-free supernatant did not show any bacteriocin activity, suggesting that the substances could be antimicrobial peptides. Solvent extraction of bacteriocin was performed by using chloroform and here maximum bacteriocin activity was found in interface layer rather than aqueous and organic layer.


Keywords


Antipathogenic metabolites; Bacteriocin; Antimicrobial peptide; Normal microflora.

Full Text:

PDF

References


Salyers Aa, Whitt Dd (2000) Microbiology: Diversity, Disease and the Environment. Bethesda Maryland: Fitzgerald Science Press. Immunol Lett. 2004 May 15;93(2-3):97-108.Commensal bacteria (normal microflora), mucosal immunity and chronic inflammatory and autoimmune diseases.

Davis Cp. (1996) Baron,s Medical Microbiology. 4th edition chapter 6. Available in http://www.ncbi.nlm.nih.gov/books/NBK7627/

Davidoliver. (2006) MICROBES AND YOU: NORMAL FLORA archive, textbook. Http://www.scq.ubc.ca/microbes-and-you-normal-flora/

Todar, K. (2013) Todar’S Online Textbook of Bacteriology, Part 2, Chapter 2, Page 2-5. Http://textbookofbacteriology.net/normalflora_2.html.

Servin LA (2004). Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens FEMS Microbiol Rev. Oct;28(4):405-40.

Chavan Ma, & Riley Ma (2007) Molecular Evolution of Bacteriocins in Gram-Negative Bacteria, In:Bacteriocins: Ecol Evolut. Pp. 5-18,

Gallo Lr, Nakatsuji T. (2011) Microbial Symbiosis with the innate immune defense system of the Skin. .J Invest Dermatol. 131, pp 1974–1980;

Walls T, Power D, Tagg J (2003) Bacteriocin-like inhibitory substance (BLIS) production by the normal flora of the nasopharynx: J. Med. Microbiol. Vol. 52 no. 9 829-833.

Sanders, E. R. Aseptic Laboratory Techniques: Plating Methods. J. Vis. Exp. (63), e3064, doi:10.3791/3064 (2012).

Florey Hw, Chain Eb, Heatly Ng, Jenninings Ma, Saunders Ag, Abraham Ep, And Florey Me (1949) Antibiot. 1:273-287.

Bauer Aw, Kirby Mm, Sherris Jc, Turuck M (1966) Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Pathol. 45(4):493-496.

Magaldi S, Mata-Essayag S, Hartung De Capriles C, Perez C, Colella Mt, Olaizola C, Ontiveros Y(2004) Well diffusion for antifungal susceptibility testing. Int J Infect Dis. Volume 8, Issue 1 , Pages 39-45.

Lima-Filho, J.V.M., A.F.F.U. Carvalho, S.M. Freitas and V.M.M. Melo, 2002. Antibacterial activity of extracts of six macroalgae from the Northeastern Brazilian Coast.Brazil. J. Microbiol., 33: 311-313

Buchanon RE. Gibson NE (1974) Bergey’s Manual of Determinative Bacteriology, 8th ed. Williams and Wilkins Co. Baltimore. Page:530-534.

Holt JG, Krieg NR, Sneath Pha, Staley JT, Williams ST (2000) Bergey’s manual of determinative bacteriology, 9th ed. Lippincott Williams and Wilkins, Baltimore. Pp-532,549-551,559,576,592.

8809911JAA(01) 2012-03 CLA COMPLEXTY:HGH CDC IDENTIFIER CODES ANALYTE:0412 TEST SYSTEM:07919

Schillinger V. Lucke Fk (1989) “Antimicrobial activity of Lactobacillus sakeisolated from meat”. Appl. Environ. Microbiol. 55; 1901-1906.

Yamamoto Y, Togawa Y, Shimosaka M, Okazaki M, (2003) Purification and characterization of a novel bacteriocin produced by Enterococcus faecalisstrain. RJ-11.App Environ Microbiol. 69, 5746–5753.

Narayanapillai Udhayashre, Duraisamy Senbagam, Balakrishnan Senthilkumar, Kanagaraj Nithya, Ramasamy Gurusamy (2012), Asian Pacific Journal of Tropical Biomedicine (2012)S406-S410, journal homepage:www.elsevier.com/locate/apjtb.

Burianek Ll, Yousef AE (2000) Solvent extraction of bacteriocins from liquid cultures. Lett Appl Microbiol.;31(3):193–197.

Chaimanee V, Sakulsingharoj C, Deejing S, Seetakoses P, Niamsup P (2009) Screening and characterisation of bacteriocin-producing bacteria capable of inhibiting the growth of bovine mastitis. Maejo Int J Sci Tech. 3(01), 43-52.

Bizani, D., Brandelli. A. 2002. Characterization of a bacteriocin produced by a newly isolated Bacillus sp. Strain 8 A. J Appl Microbiol. Volume 93, Issue 3, pages 512–519.

Fatima, D. and Mebrouk, K. 2012. Characterization and determination of the factors affecting anti-listerial bacteriocins from Lactobacillus plantarum and Pediococcus pentosaceus isolated from dairy milk products. African Journal of Food Science Vol. 7(2) pp. 35-44.

Lauková, A. Mareková, M. Javorský, P. 1993. Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231 Enterococcus faecium CCM4231. Lett in Applied Microbiol. Volume 16, Issue 5, pages 257–260.


Refbacks

  • There are currently no refbacks.


 
 
  
 

 

  


About IJSBAR | Privacy PolicyTerms & Conditions | Contact Us | DisclaimerFAQs 

IJSBAR is published by (GSSRR).