Validation of Recently Discovered mRNA Stable Regions as Biomarkers for Body Fluids after Exposure to Environmental Hazards

Taha. E, Gomaa. R, Nader. L


The recognition of messenger RNA become common practice for body fluid identification in forensic field. Though, the degraded and rare nature of RNA from forensic samples point out that mRNA transcripts are not reliably spotted or remain undetected in practice. Thus, leading to hardening the process of forensic investigation and body fluids identification. Lately, using massively parallel sequencing technique, data obtained from degraded body fluids were used to design new primers to amplify transcript regions of messenger RNA of high read coverage, hence, exhibiting higher stability, as a substitute of the conventional primer that target span exon–exon boundaries which showed low stability and degradation susceptibility. In the current project, the relative gene expression was calculated for each biomarker to measure the stability of those messenger RNA regions. The criteria of testing involved exposing different messenger RNA biomarkers transcripts of blood, buccal cells and menstrual blood to various environmental hazards including UV irradiation, 40ᵒC induced heat and 50% humidity. Degradation was in continuous exposure for a specific time span of 2 weeks. The results showed that each messenger RNA biomarker behaved differently among the different environmental insults.


body fluids; degradation; environmental hazards; mRNA transcripts.

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. Henry C. and Elaine M. (September, 2013). “Forensic Evidence and Crime Scene Investigation”. Journal of Forensic Investigation. [On-line]. 1(2). pp 1-2. Available: [July 31, 2017]

. Prakash M, Anil J. (October, 2011). “Effect of Ageing & Environmental Condition for Detection of Blood Group from Blood Stain”. Journal of Indian ACAD forensic med. [On-line]. 33(4): pp 308. Available: [August 26, 2017]

. Senay M, et al. (May, 1976). “Acclimatization of calves to a hot humid environment.” Journal of Applied Physiology. [On-line]. 40(05): pp 786–796. Available: [July 1, 2017]

. Pereira M, Martin P. (January, 1977). "Determination of Lewis Secretions in Body Fluids and Dried Stains of Body Fluids." Journal of the Forensic Science Society. [On-line]. 17(23): pp135-38. Available: [August 13, 2017]

. Bauer M. (March 2007). “RNA in forensic science.” Forensic Science International: Journal of Forensic Science International: Genetics. [On-line] 1(1): pp 69–74. Available: [August 28, 2017]

. Lin, M., Patricia P., and Rachel F. (June, 2016) "Degraded RNA transcript stable regions (StaRs) as targets for enhanced forensic RNA body fluid identification." Journal of Forensic Science International: Genetics. 20. pp 61-70. Available: [April 6, 2017]

. Livak K, Schmittgen T. (December, 2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Journal of ELSVIER. [On-line]. 25(4). pp 402-208. Available: [August 5, 2017]


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