Lead Induced Alterations in PSII and Lipid Peroxidation of Oscillatoria subbrevis and its Relevance in Bioremediation of Lead

Praveena Kumari N, Raghuram M

Abstract


In this present investigation, we have made a study on alterations in photosystem II electron transport activity and lipid peroxidation at different concentrations of lead [Pb(NO3)2] (5, 10 and 15 µM) in the thylakoid membranes of Oscillatoria  subbrevis. When the concentration of lead is increased from 5 μM to 15 μM, it gradually caused enhancement in the lipid peroxidation from 34 n moles up to 43 n moles concentration. At 15 µM Pb concentration in the medium 43% of enhancement in lipid peroxidation was noticed. This heavy metal (Pb) induced lipid peroxidation is responsible for the damage of PS II and its related electron transport activity. An investigation was done to know the protective action of ascorbate to minimize the lipid peroxidation, by addition of 8µM concentration of ascorbate in the medium with 15µM concentration of lead heavy metal. The results has shown that Ascorbate acts as a protective agent to minimize lipid peroxidation and enhance photosynthetic activity which will help in the better survival of these cyanobacterial species and their promising use in the bioremediation of   industrial effluents.


Keywords


Bioremediation; Cyanobacteria; Lipid peroxidation; Electron transport activities.

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