Lead Induced Alterations in PSII and Lipid Peroxidation of Oscillatoria subbrevis and its Relevance in Bioremediation of Lead

Praveena Kumari N, Raghuram M


In this present investigation, we have made a study on alterations in photosystem II electron transport activity and lipid peroxidation at different concentrations of lead [Pb(NO3)2] (5, 10 and 15 µM) in the thylakoid membranes of Oscillatoria  subbrevis. When the concentration of lead is increased from 5 μM to 15 μM, it gradually caused enhancement in the lipid peroxidation from 34 n moles up to 43 n moles concentration. At 15 µM Pb concentration in the medium 43% of enhancement in lipid peroxidation was noticed. This heavy metal (Pb) induced lipid peroxidation is responsible for the damage of PS II and its related electron transport activity. An investigation was done to know the protective action of ascorbate to minimize the lipid peroxidation, by addition of 8µM concentration of ascorbate in the medium with 15µM concentration of lead heavy metal. The results has shown that Ascorbate acts as a protective agent to minimize lipid peroxidation and enhance photosynthetic activity which will help in the better survival of these cyanobacterial species and their promising use in the bioremediation of   industrial effluents.


Bioremediation; Cyanobacteria; Lipid peroxidation; Electron transport activities.

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DJ Thiele “Metal detoxification in eukaryotic cells”. Crisp. Data Base of national Institute of Health. Washington D.C., 1995.

M Megharaja, S Avudainayagam, R Naidu “Toxicity of hexavalent chromium and its reduction by bacterium isolated from soil contaminated with tannery waste”. Curr Microbial., 2003; 47: 51-54.

KB Chekroun, M Baghour “The role of algae in phytoremediation of heavy metals: A review”. J Mater Environ Sci., 2003; 4(6): 873-880.

S Dwivedi “Bioremediation of heavy metals by algae- Current and future perspective”. J Adv Lab Res Biol., 2012; 3(3): 195-199.

C Hong, P Shan-Shan “Bioremediation potential of Spirullina sp. toxicity and bio sorption studies of lead”. Journal of Zhejiang Univ Sci., 2005; 6B (3): 171-174.

K Chojnacka , A Chojnacki ,H Goreck “Biosorption of Cr2+, Cd2+, Cu2+ ions by blue green algae”. Aquatic Toxicol. 1997; 39: 231-246.

JM Pisciotta, Y Zou and Baskakov IV. “Light-Dependent Electrogenic Activity of Cyanobacteria”. PLoS ONE . 2010 (5) e10821:1- 10.

A.S. Modaihs, Al-Swailem, M.S Mahjoubs. . “Heavy metals content of commercial inorganic fertilizers used in the Kingdom of Saudi Arabia”. , Sultan Qaboos Univ. Agric. Marine Sci., 2011, 9(1): 21-25.

R. Rippka, J. Deruelles, J.B. Waterbury, M. Herdman and R.Y. Stainer .” Generic assignments, strain histories and properties of pure cultures of cyanbacteria”. J. Gen.Microbiol., 1979, 111:1-61.

S. Katoh “The discovery and function of plastocyanin: A personal account of Photosynthesis.” 1997, 43:177-189.

S.D.S Murthy “Studies on bioenergetics processes of cyanobacteria: Analysis of the selected heavy metal ions on energy linked process.” Ph. D thesis, Jawaharlal Nehru University, New Delhi., 1991.

O. Warburg and Luthgens, W . Nature, 1994, 32: 161.

A.Trebst. and E.Pistorius, . Z. Natwforsch. 1965, 20: 143-147.

R.L. Health and Packer . “Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation”. Archiv. Biochem. Biophy. 1968, 125: 189-198

Y Nishiyama , H Yamamoto ,SI Allakhverdiev ,M Inaba , AYokota & N Murata “Oxidative stress inhibits the repair of photodamage to the photosynthetic machinery”. 2001, EMBO J 20: 5587–5594.

M. J Quiles, N. I. López, .”Photoinhibition of photosystems I and II induced by exposure to high light intensity during oat plant grown effects on the chloroplastic NADH dehydrogenase complex”, Plant Science, 2004, 166, 815-823.

Meenakshi Choudhary, Umesh Kumar, J,Mohammed Abash Khan, Sunaina Zutshi, . “Size, temperature and membrane lipid composition”. J. Biol.Chem. 2007, 276, 46830-46834.

S Gupta Singh. S, Sharma, Sunitha.. “Tolerence against heavy metal toxicity in cynobacteria: Role of antioxidant defence system”. International Journal of Pharmacy and Pharmaceutical Sciences. 2015 , 7(2): 9-16.


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