The Effect of Cymbopogon citratus Essential Oil-Water Emulsion on Some Blood Parameters and Histopathology of Liver and Kidney in Mice

Abinet Gebremickael, Girmai Gebru, Asfaw Debella, Getachew Addis, Eyasu Makonnen, Yonas Bekuretsion


This study was aimed to assess the possible toxic effects of Cymbopogon citratus essential oil-water emulsion after sub chronic oral administration in mice.

The essential oil extraction of the leaves of C.citratus was carried out through hydro-distillation technique. In this sub-chronic toxicity evaluation, two treatment groups of mice each containing twelve animals were used. Two test doses, 1.5 and 2.5ml/kg, were selected based on the earlier findings of acute toxicity study on similar emulsion in mice and administered to the treatment groups. The control group animals were received vehicle. At the end of 12 consecutiveweek sub-chronic administration, blood samples were taken by cardiac puncture and then, all animals were sacrificed and the vital organs were collected for gross and histopathological examinations. No death was recorded, and there was no significant change in the evaluated hematological and biochemical parameters after the sub-chronic oral administration in mice. Besides, there was no obvious change in sections of the liver and kidneys of mice treated at 1.5ml/kg dose. However, some pathological changes were observed in liver sections of mice treated at 2.5ml/kg dose. The emulsion at and below 2.5ml/kg body weight doses does not produce obvious toxic effects after sub chronic treatment in mice. Further toxicological investigation is however, needed to confirm this.


Hydro-distillation; Cymbopogon citratus; Emulsion; Sub-chronic; Essential oil.

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