Coliform Bacteria and Hatching Egg Disinfectants

Tarek M. Mousa-Balabel, Samy A. Al-Midani, Moustafa A. Al-Refaay, Shimaa M. Kerady

Abstract


(This study aimed to monitoring the microbial status of broiler breeder hatching eggs through isolation of coliform bacteria, especially Salmonella and E coli from eggs and hatched chicks and identification of isolated microorganism by PCR. Besides, the reduction of the contamination rate by using different egg disinfectants. A total of 600 broiler breeder Saso fertile eggs was purchased from a commercial broiler breeder flock aged 27 wk, which reared on a deep litter system at a private farm in Gharbiya Governorate, Egypt. The eggs were allocated randomly into four equal treatment groups (n?150) according to the disinfectant used. The eggs in the first group were kept as a control without treatment. While, the eggs in the second group were disinfected by 1.4% H2O2. The eggs in the third group were disinfected by 0.5% TH4. The eggs in the fourth group were disinfected by 0.5% Virkon S. Results revealed that, the hatchability percent was increased by applying the different egg disinfectants. The highest total embryonic deaths were recorded in eggs disinfected by TH4 in comparison to other disinfectants used. The weak chicks' quality appeared only in eggs disinfected by H2O2 and TH4 but the highest percent of omphalities was recorded in eggs disinfected by H2O2 followed by the control group. Egg disinfectants were significantly reduced the eggshell total bacterial count from 27 Logs to 4.3 Logs with a 84.08% reduction by using H2O2, from 24 Logs to 4.7 Logs with a 80.42% reduction after using virkon S and reduced the total bacterial count from 25 Logs to 3 Logs with a 88% reduction after using TH4 in comparison to 28 Logs for the control group. PCR findings showed that salmonella and Escherichia coli were the predominant bacteria on the surface of the eggshell. The prevalence of Escherichia coli was the highest, followed by salmonella. It could be concluded that E. coli is more predominant than salmonella).



Keywords


bacterial count; hatchability; embryonic mortality; salmonella; E. coli.

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References


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