Coliform Bacteria and Hatching Egg Disinfectants

Tarek M. Mousa-Balabel, Samy A. Al-Midani, Moustafa A. Al-Refaay, Shimaa M. Kerady


(This study aimed to monitoring the microbial status of broiler breeder hatching eggs through isolation of coliform bacteria, especially Salmonella and E coli from eggs and hatched chicks and identification of isolated microorganism by PCR. Besides, the reduction of the contamination rate by using different egg disinfectants. A total of 600 broiler breeder Saso fertile eggs was purchased from a commercial broiler breeder flock aged 27 wk, which reared on a deep litter system at a private farm in Gharbiya Governorate, Egypt. The eggs were allocated randomly into four equal treatment groups (n?150) according to the disinfectant used. The eggs in the first group were kept as a control without treatment. While, the eggs in the second group were disinfected by 1.4% H2O2. The eggs in the third group were disinfected by 0.5% TH4. The eggs in the fourth group were disinfected by 0.5% Virkon S. Results revealed that, the hatchability percent was increased by applying the different egg disinfectants. The highest total embryonic deaths were recorded in eggs disinfected by TH4 in comparison to other disinfectants used. The weak chicks' quality appeared only in eggs disinfected by H2O2 and TH4 but the highest percent of omphalities was recorded in eggs disinfected by H2O2 followed by the control group. Egg disinfectants were significantly reduced the eggshell total bacterial count from 27 Logs to 4.3 Logs with a 84.08% reduction by using H2O2, from 24 Logs to 4.7 Logs with a 80.42% reduction after using virkon S and reduced the total bacterial count from 25 Logs to 3 Logs with a 88% reduction after using TH4 in comparison to 28 Logs for the control group. PCR findings showed that salmonella and Escherichia coli were the predominant bacteria on the surface of the eggshell. The prevalence of Escherichia coli was the highest, followed by salmonella. It could be concluded that E. coli is more predominant than salmonella).


bacterial count; hatchability; embryonic mortality; salmonella; E. coli.

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A. M. Ahmed? Y. Motoi; M. Sato; A. Marayama; H. Watandabe; Y. Fukumoto, and T. Shimamoto, "Zoo animals as a reservoir of Grame-negative bacteria harboring integrons ana antimicrobial resistance genes". App. Environ. Microbial. 73: 6686-6690, 2007.

F. T. M. Beleh, "Effects of various sanitizing treatments on hatchability of broiler breeder eggs". M. Sci. Thesis, Fac. of Graduate Studies., Najah National Univ. Nablus, Palestine, 2008.

R. K.. Bramwell, "Importancia de lasprcticas de manejo de lscasetas de reprodutoras". IndstriaAvcola., 47: 8-18, 2000.

S. Cadirci, Disinfection of hatching eggs by formaldehyde fumigation a review. Arch. Geflgelk., vol. 73, pp. 116-123, 2009.

H. Christensen; M. Bisgaard; A. M. Bojesen; R. Mutters, and J. E. Olsen, "Genetic relationships among avian isolates classified as Pasteurella haemolytica, 'Actinobacillus salpingitidis' or Pasteurella anatis with proposal of Gallibacterium anatis gen. nov., comb. nov. and description of additional genomospecies within Gallibacterium gen. nov.". Int. J. Syst. Evol. Microbiol., 53:275-287, 2003.

M. I. Cook; S. R Beissinger; G. A. Toranzos, and W. J. Arendt, Microbial infection affects egg viability and incubation behavior in a tropical passerine. Behav. Ecol., vol. 16, pp. 30 36, 2005.

N. A. Cox; M. E. Berrang, and J. A. "Salmonella penetration of egg shells and proliferation in broiler hatching eggs. A review". Poult. Sci., 79: 1571-1574, 2000.

ISO 6579, "Microbiology of food and animal feeding stuffs-horizontal method for the detection of Salmonella species". International Standard, (4th edition), 2002.

M. Lyutskanov; V. Urumova, and G. Zhelev, "Comparative evaluation of the efficacy of various sanitizers in a poultry hatchery". Bulgarian Journal of Veterinary Medicine, 13, no 2, 111?116, 2010.

A. J. Mansour, "Hydrogen peroxide a versatile disinfectant". Poult. Int. 8, 4445, 2001.

R. Meijerhof, "Embryo temperature as a tool in the incubation process". Incubation and Fertility Research Group (WPSA Working Group 6 (Reproduction), St. Edmunds Hall, Oxford, UK, 2000.

L. Milakovic-Novak, and L. Prukner, "Hygiene levels of eggs". Options Mditerranennes, Series A, 7: 239-242, 1990.

T. M. Mousa-Balabel; R. A. Mohamed; S. A. Al-Midani, and Maha SH. Abd El-Samad, "Impact of Boiler Breeders Hatching Eggs Disinfection Time on Some Hatchability Parameters". International Journal of Sciences: Basic and Applied Research (IJSBAR), 30(2): 230-240, 2016.

F. Olayemi, and C. Adetunji, "Effect of rinses on microbial quality of commercially available eggs and its components before processing from Ilorin in western Nigeria". Bitlis Eren Univ. J. Sci. Technol., 3: 44-47, 2013.

J. Quinn; K. Markey; E. Carter; J. Donnelly, and C. Leonard, "Veterinary Microbiology and Microbial Diseases". Black well scientific publication, Oxford, London, 2002.

P. A. Renner; K. E. Nestor, and.G. B. Havenstein, "Effects on turkey mortality and body weight of type of beak trimming, age at beak trimming, and injection of poults with vitamin and electrolyte solution at hatching". Poult. Sci., 68:369373, 1989.

J. D. Rodgers; J. J. McCullagh; P. T. C. McNamee; A. Bel; N. Brice; J. A. Smyth, and. H. J. Ball, "An investigation into the efficacy of hatchery disinfectants against strains of StaphyLococcusaureus associated with the poultry industry". Vet. Record., 153: 656-657, 2003.

T. A. Scott, and C. Swetnam, "Screening sanitizing agents and methods of application for hatching eggs". 1. Environmental and user friendliness. J. Appl. Poult. Res., 2: 16, 1993a.

T. A. Scott, and C. Swetnam, "Screening sanitizing agents and methods of application for hatching eggs". 2. Effectiveness against microorganisms on the egg shell. J. Appl. Poult. Res., 2: 711, 1993b.

B. W. Sheldon, and J. Brake, "Hydrogen peroxide as an alternative hatching egg disinfectant". Poultry Sci., 70: 1092-1098, 1991.

D. Swayne; E. Charman; J. R. Glisson; M. W. Jackwood; J. E. Pearson, and W. M. Reed, "Colibacillosis: A laboratory manual for the isolation and identification of avian pathogens", 4th Edition. Am. Assoc. Avian Pathol., 14-16, 1998.

I. Y?ld?r?m, and M. Ozcan, "Use of oregano and cumin essential oils as disinfectant on hatching Quail eggs". J. Anim. Res. Inst., 1: 6163, 2001.


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