In Vitro Selection of Drought Stress Rice (Oryza sativa L) Varieties Using PEG (Polyethylene Glycol)

Nurhayati Nurhayati, Murni Sari Rahayu, Irham Syaukani, Sri Hafnida Ritonga

Abstract


The research was conducted as long as February to April 2016 at Laboratory of Tissue Culture, UPT of  Parent Seed Centre (BBI), Johor Building, Agriculture Office, Provincial Government of  North Sumatera. The purpose of the study is to obtain in vitro drought tolerant varieties. The research design is factorial completely randomized design (DCA) with two factors and three  replications.  The first factor is varieties (V), consisting of four varieties namely V1 = Batutegi, V2 = Jatiluhur, V3 = Sigambiri Putih, and V4 = Sigambiri Merah.  The second factor is PEG concentration of 6000 (P), which consists of four levels namely P0 = 0%, P1 = 8%, P2 = 16%, and P3 = 24%. The observed variables are plantlets height (cm), number of leaves (sheet), root length (cm), number of roots (roots) and proline content (%).

Jatiluhur variety is drought-tolerant because show the best growth in PEG treatment at concentration 24%.  Growth of plantlets height, number of leaves, and number of roots and proline content of Jatiluhur is the highest at concentration of PEG 24% so that this variety is more tolerant than other varieties by vitro test.

 


Keywords


Drought Stress, PEG, rice varieties, In Vitro

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