The Relationship between VDR Gene mRNA Expression and Incidence of Stillbirth

Budy Utomo, Andi Zulkifli Abdullah, Ansariadi Ansariadi, Sukri Palutturi, Mohammad Hatta, Andi Nilawati Usman

Abstract


More than 2.65 million stillbirths occur each year worldwide, or over 7,300 stillbirths occur every day. Vitamin D has an important role during pregnancy, such as facilitating transport some nutrients through the placenta, including calcium metabolism and modulation of the immune response as well asit is an essential nutritional factor for the health of the mother during pregnancy and the fetus. The study aims to elucidate the relationship between VDR genemRNA expression andincidence of stillbirth. This study used a case-control design. Sample size of this study was68 respondents that consistedof 34 respondents for the control group and 34 respondents for the case group with a ratio of 1:1. The sampling method referred to the formula sample size determination in health studies. Odds ratio was determined by analyzing several previous studies. The case group comprised pregnant mothers who experienced stillbirth, whereas, the control group included respondents who delivered live births and had fulfilled the inclusion and exclusion criteria. Data were analyzed using chi-square formula (? = 0.05) with the determination of odds ratio (OR).

The study indicated that mean value ofVDR gene mRNA expression was higher in the group of respondents who did not experience stillbirth. Results of the ANOVA test showed that there wasstatistically significant difference of VDR gene mRNA expression in the two groups (p = 0.000). Low VDR gene mRNA expression caused higher risk of stillbirth in the case group (79.4%) than the control group (41.2%), whereas, high VDR gene mRNA expression caused lower risk of stillbirth in the control group (58.8%) than the case group (20.6%).Results of the data analysis showed that the value of chi-square test was p = 0.003 (p <0.05), and hence, there was statistically significant correlation between VDR gene expression mRNA and incidence of stillbirth. Results of the statistical analysis revealed that the value of OR was 5.51 (95% CI = 1.87 to 16.15). It was concluded that respondents with low VDR gene mRNA expression (<mean value) had 5.5 times more likely had incidence of stillbirth than those with high VDR gene expression mRNA (? mean value).


Keywords


Stillbirth; Vitamin D Receptor Gene; VDR Gene mRNA expression.

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References


WHO. 2011. PMNCH Fact sheet: Stillbirths. by The Partnership for Maternal, Newborn & Child Health. World Health Organization.

Lawn, J.E., Blencowe, H., Pattinson, R., Cousens, S., Kumar, R., Ibiebele, I. & Stanton, C. 2011. Stillbirths: Where? When? Why? How to make the data count?. The Lancet, 377(9775), 1448-1463.

The 2012 Indonesia Demographic and Health Survey (IDHS). Report of Statistics Indonesia, August 2013.

Regional Health Department of Southeast Province,2016. The 2015 Health Profile of Southeast Province. Kendari. (in Indonesian languange)

Fretts, R.C. 2011. Incidence, etiology, and prevention of stillbirth. (http://cursoenarm.net/UPTODATE/contents/mobipreview.htm?32/61/33745, diakses 20 juli 2015).

Wou, K., Ouellet, M.P., Chen, M.F., & Brown, R.N. 2014. Comparison of the aetiology of stillbirth over five decades in a single centre: a retrospective study. BMJ open, 4(6), e004635.

Olmos-Ortiz, A., Avila, E., Durand-Carbajal, M., & Daz, L. 2015. Regulation of calcitriol biosynthesis and activity: focus on gestational vitamin D deficiency and adverse pregnancy outcomes. Nutrients, 7(1), 443-480.

Aghajafari, F., Nagulesapillai, T., Ronksley, P.E., Tough, S.C., OBeirne, M., & Rabi, D.M. 2013. Association between maternal serum 25-hydroxyvitamin D level and pregnancy and neonatal outcomes: systematic review and meta-analysis of observational studies. Bmj, 346, f1169.

Liu, N.Q., Kaplan, A.T., Lagishetty, V., Ouyang, Y.B., Ouyang, Y., Simmons, C.F., & Hewison, M. 2011. Vitamin D and the regulation of placental inflammation. The Journal of Immunology, 186(10), 5968-5974.

Hollis, B.W., & Wagner, C.L. 2011. Vitamin D requirements and supplementation during pregnancy. Current Opinion in Endocrinology, Diabetes and Obesity, 18(6), 371-375.

Pratumvinit, B., Wongkrajang, P., Wataganara, T., Hanyongyuth, S., Nimmannit, A., Chatsiricharoenkul, S., & Reesukumal, K. 2015. Maternal Vitamin D Status and Its Related Factors in Pregnant Women in Bangkok, Thailand. PloS one, 10(7), e0131126.

Lawn, J.E., Yakoob, M.Y., Haws, R.A., Soomro, T., Darmstadt, G.L., & Bhutta, Z.A. 2009. 3.2 million stillbirths: epidemiology and overview of the evidence review. BMC pregnancy and childbirth, 9(Suppl 1), S2.

Urrutia, R.P. & Thorp, J.M. 2012. Vitamin D in pregnancy: current concepts. Current opinion in obstetrics & gynecology, 24(2), 57.

Kaushal, M., & Magon, N. 2013. Vitamin D in pregnancy: A metabolic outlook. Indian journal of endocrinology and metabolism, 17(1), 76.

Pike, J.W and Meyer, M.B. 2010. The Vitamin D Receptor: New Paradigms for the Regulation of Gene Expression by 1,25-Dihydroxyvitamin D3. Endocrinol Metab Clin North Am. 39 (2): 255-269.

Prietl B., Treiber G., Pieber T.R and Amrein K. 2013. Vitamin D and Immune Function. Nutrients. 5. 2502-2521

Lagishetty V., Liu N.Q., Hewison M. 2011. Vitamin D metabolism and innate immunity. Mol Sel Endocrinol. 347 (1-2): 97-105.

Setiabudiawan, B. 2010. Deficiency of Vitamin D and Polymorphisms of VDR Gen Variants of FokI, BsmI, ApaI and TaqI on Tuberculosis among Children. Sari Pediatri, 11(5): 317-325. (in Indonesian language)

Manzon, L., Altarescu, G., Tevet, A., Schimmel, M.S., Elstein, D., Samueloff, A., & Grisaru-Granovsky, S. 2014. Vitamin D receptor polymorphism FokI is associated with spontaneous idiopathic preterm birth in an Israeli population. European Journal of Obstetrics & Gynecology and Reproductive Biology, 177, 84-88.

Bukowski, R., Hansen, N.I., Willinger, M., Reddy, U.M., Parker, C.B., Pinar, H., & Koch, M.A. 2014. Fetal growth and risk of stillbirth: a population-based case-control study. PLoS Med, 11(4), e1001633.

Hulthn Varli, I., Petersson, K., Kublickas, M., & Papadogiannakis, N. 2012. Both acute and chronic placental inflammation are overrepresented in term stillbirths: a case-control study. Infectious diseases in obstetrics and gynecology, ID 293867, 8.

Clancy, N., Onwuneme, C., Carroll. A., McCarthy, R., McKenna, M.J., Murphy, N. 2013. Vitamin D and neonatal immune function. J Matern Fetal Neonatal Med, 26: 639-646.

Shin, J.S., Choi, M.Y., Longtine, M.S., & Nelson, D.M. 2010. Vitamin D effects on pregnancy and the placenta. Placenta, 31(12), 1027-1034.

Adams, J.S & Hewison, M. 2008. Unexpected actions of vitamin D: new perspectives on the regulation of innate and adaptive immunity. Nat Clin Pract Endocrinol Meta. 4(2): 8090.

Hassan, J & Connell, J. 2007. Translational Mini?Review Series on Infectious Disease: Congenital cytomegalovirus infection: 50 years on. Clinical & Experimental Immunology, 149(2), 205-210.

Montoya, J.G & Remington, J.S. 2008. Management of Toxoplasma gondii infection during pregnancy. Clinical Infectious Diseases, 47(4), 554-566.

McClure, E.M., Dudley, D.J., Reddy, U., & Goldenberg, R.L. 2010. Infectious causes of stillbirth: a clinical perspective. Clinical obstetrics and gynecology, 53(3), 635.

Murthi, P., Yong, H., Ngyuen, T., Ellery, S., Singh, H., Rahman, R. 2016. Role of the Placental Vitamin D Receptor in Modulating Feto-Placental Growth in Fetal Growth Restriction and Preeclampsia-Affected Pregnancies. Front Physiol, 7: 43.

Bouillon, R., Okamura, W.H., Norman, A.W. 1995. Structure-function relationships in the vitamin D endocrine system. Endocr Rev, 16 (2): 200-257.

Rasmussen, H., and Anast, C. 1983. Familial hypophosphatemic rickets and vitamin D dependent rickets. In: Stanbury, J.B., Wyngaarden, J.B., Fredrickson, D.S., Gold-stein, J.L and Brown, M.S. eds. The metabolic basis of inherited disease. 5th ed. McGraw-Hill, New York. 1743-1773.

Gogate, S. 2001. Intra-uterine growth restriction-obstetricians perspective Int. J. Diab. Dev-Countries, vol 21, 51-55.

Cnattingius, S., Bergstrm, R., Lipworth, L., and Kramer, M.S. 1998. Prepregnancy Weight and the Risk of Adverse Pregnancy Outcomes. N Engl J Med, 338:147-152.


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