Detection Salmonella Typhi RNA from Suspect Typhoid using Reverse Transcription-Polymerase Chain Reaction

Fias Fias, Mochammad Hatta, Andi Asadul Islam, Rahmawati Minhajat, Maryunis Maryunis

Abstract


In endemic areas, approximately 90% of enteric fever is typhoid fever. Diagnosis of typhoid fever is difficult to be enforced only on the basis of clinical symptoms alone, because the clinical features of this disease is very varied and generally not typical. Thus the role of the laboratory in assisting the diagnosis is very important. The aim of this research is to detection of Salmonella typhi from suspected typhoid RNA using Reverse Transcription-Polymerase Chain Reaction. The design of this study is cross-sectional analytical survey. The total sample of 101 patients. Data analysis was done using the Chi square test with a confidence level of 95%. All the data were Analyzed by SPSS software, version 21.0 (SPSS, Inc., Chicago, IL). The result showed no association temperature with the results of PCR (p = 0.004), there is a correlation incident dirty tongue with the results of the PCR (p = 0.000), there is a relationship incidence of headache with the results of the PCR (p = 0.027), there is a relationship incidence of dizziness the results of the PCR (p = 0.035), and no association epistaxis events with the results of the PCR (p = 0.024).

As for the other characteristics: age (p = 0.226), gender (p = 0.819), duration of fever (p = 0.268), fever afternoon (p = 0.579), fever continuously (p = 0.389), cough (p = 1.000), insomnia (p = 0.631), decreased consciousness (p = 1.000), psychosis (p = 1.000), weak (p = 0.314), nausea (p = 1.000), abdominal pain (p = 0.547), anorexia (p = 0.266), constipation (p = 1.000), and diarrhea (p = 0.512) was not associated with the results of PCR. In conclusion, there is a relationship of temperature, dirty tongue, headache, dizziness, and epistaxis with positive PCR test results.


Keywords


PCR; Salmonella typhi; Reverse Transcription-Polymerase Chain Reaction.

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References


Suharto. (2015). Demam Tifoid. Dalam A. Tjokoprawiro, P. B. Setiawan, C. Effendi, & D. Santoso, Buku Ajar Ilmu Penyakit Dalam (hal. 647-652). Surabaya: Airlangga University Press.

Pegues, D. A., & Miller, S. I. (2015). Salmonellosis. Dalam D. L. Kasper, A. S. Fauci, S. L. Hauser, D. L. Longo, & Jameson, Harrisons Principles of Internal Medicine (hal. 1049-1053). New York: International Edition< McGraw-Hill.

Hatta, M., Mubin, H., Abdoel, T., Smits, & Henk, L. (2002). Antibody Response in Typhoid Fever in Endemik Indonesia and the Relevance of Serology and Culture to Diagnosis. Southeast Asian Journal Tropical Medicine and Public Health , Vol 33.

Sabir, M., Yadi, Firdaus, & Hatta, M. (2003). Perbandingan Tes Serologi Dipstik dengan Widal Untuk Diagnosis DT. Jurnal Kedokteran Trisakti , vol. 22, no.3.

Pastoor, R., Hatta, M., Abdoel, & Smith, H. L. (2008). Simple, Rapid, and Affordable Point-of-Care Test for the Serodiagnosis of Typhoid Fever. Diagnostic Microbiology and Infectious Disease , 129-134.

Hatta, M., & Smits, H. (2007). Detection of Salmonella Thypi by Nested Polymerase Chain Reaction in Blood, Urine and Stool Samples. Am J.Med.Hyg , 76(1):139-143.

Thriemer, K., Ley, B. B., Ame, S. S., Deen, J. L., Pak, G. D., Chang, N. Y., et al. (2012). Clinical and Epidemiological Features of Typhoid Fever in Pemba, Zanzibar: Assessment of the Performance of the WHO Case Definitions. plosone , http://dx.doi.org/10.1371/journal.pone.0051823.

Adhikari, A., Rauniyar, R., Raut, P. P., Manandhar, K. D., & Gupta, B. P. (2015). Evaluation of sensitivity and specificity of ELISA against Widal test for typhoid diagnosis in endemic population of Kathmandu. BMC Infectious Diseases , 15:523 DOI: 10.1186/s12879-015-1248-6.

Mitra, R., Bhan, S., Nath, G., Kumar, N., & Ali, Z. (2013). Development of a Novel Rapid Immunodiagnostic Kit Based on Flagellar 40 kDa Antigen Epitope for the Detection of Typhoid Fever in Indian Patients. The Scientific World Journal , ID 363652, 7 pages, 2013. doi:10.1155/2013/363652.


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