Alteration of Phagocytic Activity of White Shrimp Litopenaeus vannamei (Boone, 1934) Reared in Isosmotic Salinity and Different Density

Arifuddin Arifuddin, Alexander Rantetondok, Dody Dh Trijuno, Hilal Anshary


Effect of stocking density to assess phagocytic activity changes of white shrimp has been carried out. White shrimp reared at a salinity of 25 with five levels of density that is 150, 250, 350, 450, and 550 shrimps/m3, with three replications. Phagocytic activity of hemocytes cells was analyzed at time 0, 24, 48, 96, and 144 hours after exposure to different density. The phagocytic activity data were analyzed by repeated measurement analysis of variance at the 5% significance level. Phagocytic of the density of 150, 250, and 350 shrimps/m3 showed significant difference with a density of 450 and 550 shrimps/m3. Phagocytic activity decreased fastest occur at the level densities of 450 and 550 shrimps/m3 which is at the 24 hours after the treatment and recovery of the 144 hours, while the level of density of 350 shrimps/m3 decreased activity at 96 hours after the treatment, and the level of density of 150 and 250 shrimps/m3 relatively stable. These findings indicate that increasing stocking density increased phagocytosis activity disorder and disturbance density against phagocytic activity occurs faster in high population density.


White shrimp; Litopenaeus. vannamei; phagocytic activity; stress; density.

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C.-I. Yu and Y.-L. Song: "Outbreaks of Taura syndrome in pacific white shrimp Penaeus vannamei cultured in Taiwan," Fish Pathol., Vol. 35, no. 1, pp. 21-24, 2000.

C. Liu and J. Chen, "Effect of ammonia on the immune response of white shrimpLitopenaeus vannamei and its susceptibility to Vibrio alginolyticus," vol. 16, no. June 1985, pp. 321-334, 2004.

S. Soto-Rodriguez, B. Gomez-Gil, and R. Lozano, '' Bright-red 'syndrome in Pacific white shrimp Litopenaeus vannamei is the caused by Vibrio harveyi, "Dis.Aquat. Organ., Vol. 92, no. 1, pp. 11-19, 2010.

A. Menz and BF Blake, "Experiments on the growth of Penaeus vannamei Boone," J. Exp. Mar. Bio. Ecol., Vol. 48, no. 2, pp. 99-111, 1980.

FL Castille, "The effect of salinity on the osmotic, sodium and chloride concentration in the hemolymph of euryhaline shrimp of the genus Penaeus," vol. 68, 1981.

J. Shakir, C, Manilal, A, Jayakumari, M, Sujith, S, Selvin, "Current status of shrimp farming in the southwestern coast (Manroe Island, Kollam) of India," Iran. Ener J Env., Vol. 1, pp. 287-292, 2010.

J.. Lawrence, AL, McVey, JP, Huner, "Penaeid shrimp culture," in crustacean and mollusk aquaculture in the UnitedStates,1985, pp. 130-135.

H. Clifford, "Semi-intensive sensation: A case study in marine shrimp pond management," J. World Aquacult.Soc,vol. 25, pp. 3-6, 1994.

W. Wasielesky, H. Atwood, A. Stokes, and CL Browdy, "Effect of natural production in a zero exchange suspended microbial floc based super-intensive culture system for Litopenaeus vannamei white shrimp," vol. 258, pp. 396-403, 2006.

SJ Arnold, MJ Sellars, PJ rattle, and GJ Coman, "An evaluation of stocking density on the intensive production of juvenile brown tiger shrimp (Penaeus esculentus)," Aquaculture,vol. 256, no. 1-4, pp. 174-179, 2006.

D. Montero, "High stocking density produces crowding stress altering some physiological and biochemical parameters in gilthead seabream, Sparus aurata, juveniles," no. August, 2015.

E. Aksakal, D. Ekinci, O. Erdogan, S. Beydemir, Z. Alim, and SB Ceyhun, "Increasing stocking density causes inhibition of metabolic-antioxidant enzymes and elevates the mRNA levels of heat shock protein 70 in rainbow trout, "livest.Sci., Vol. 141, no. 1, pp. 69-75, 2011.

MW Johansson, P. Keyser, Sritunyalucksana K., and K. Sderhll, "Crustacean haemocytes and haematopoiesis," Aquaculture,vol. 191, no. 1-3, pp. 45-52, 2000.

CBT van de Braak, a Botterblom MH, N. Taverne, WB van Muiswinkel, JHWM Rombout, and WPW van der Knaap, "The roles of haemocytes and the lymphoid organ in the clearance of injected Vibrio bacteria in Penaeus monodon shrimp., "FishShellfish Immunol., Vol. 13, no. 4, pp. 293-309, 2002.

A. Tsing, J.-M. Arcier, and M. Brehelin, "Hemocytes of Penaeid and Palaemonid shrimps: Morphology, cytochemistry, and hemograms," J. Invertebr. Pathol., Vol. 53, no. 1, pp. 64-77, 1989.

P. Amparyup, W. Charoensapsri, and A. Tassanakajon, "Fish and Shell fi sh Immunology prophenoloxidase system and its role in shrimp immune responses against major pathogens," Fish Shellfish Immunol., Vol. 34, no. 4, pp. 990-1001, 2013.

AI Campa-Cordova, NY Hernandez-Saavedra, R. DE Philippis, and F. Ascencio, "Generation of superoxide anion and SOD activity in haemocytes and muscle of American white shrimp (Litopenaeus vannamei) as a response to -glucan and sulphated polysaccharide, "FishShellfish Immunol., Vol. 12, pp. 353-366, 2002.

RP Ellis, H. Parry, JI Spicer, TH Hutchinson, RK Pipe, and S. Widdicombe, "immunological function in marine invertebrates: Responses to environmental perturbation," Fish Shellfish Immunol., Vol. 30, no. 6, pp. 1209-1222, 2011.

B. Barton, C. Schreck, and L. Barton, "Effects of chronic administration and daily cortisol, acute stress on growth, physiological conditions, and stress responses in juvenile rainbow trout," Dis. Aquat. Organ., Vol. 2, pp. 173-185, 1987.

D. Montero, "Effect of dietary vitamin E and C supplementation on some immune parameters of gilthead seabream Sparus aurata / juveniles subjected to crowding stress," pp. 269-278, 1999.

TG Pottinger, "The effect of stress and exogenous cortisol on receptor-like binding of cortisol in the liver of rainbow trout, Oncorhynchus mykiss," Gen. Comp. Endocrinol., Vol. 78, no. 2, pp. 194-203, 1990.

TR Gregory and CM Wood, "Individual variation and interrelationships between swimming performance, growth rate, and feeding in juvenile rainbow trout (Oncorhynchus mykiss)," Can. J. Fish. Aquat. Sci., Vol. 55, no. 7, pp. 1583-1590, 1998.

P. Angelidis, F. Baudin-Laurencin, and P. YOUINOU, "Phagocyte Chemiluminescence, Circulating leucocytes and," vol. 31, pp. 113-122, 1987.

CC Li, ST Yeh, and JC Chen, "innate immunity of the white shrimp Litopenaeus vannamei weakened by the combination of a Vibrio alginolyticus injection and low-salinity stress," Fish Shellfish Immunol., Vol. 28, no. 1, pp. 121-127, 2010.

DP Anderson and AK Siwicki, Basic hematology and serology for fish healthprograms.Manila, Phillipines: Fish Health Section, Asian Fisheries Society, 1995.

A. Petrie and P. Watson, Statistic for veterinary and animalscience.UK: Blackwell Science.

J. Rodrguez and G. Le Moullac, "State of the art of immunological tools and health control of penaeid shrimp," Aquaculture,vol. 191, no. 1-3, pp. 109-119, 2000.

L. Cerenius, P. Jiravanichpaisal, HP Liu, and I. Sderhll, "Crustacean immunity," Adv. Exp. Med. Biol., Vol. 708, no. 12, pp. 239-259, 2010.

Barton BA, ATC Oj, and GK Zwama, "Physiological CHANGES IN FISH FROM STRESS IN AQUACULTURE WITH EMPHASIS ON THE RESPONSE AND EFFECTS OF corticosteroids," no. 6, pp. 3-26, 1991.

AD TG Pickering and Pottinger, "Stress responses and disease resistance in salmonid fish: Effects of chronic elevation of plasma cortisol," Fish Physiol. Biochem., Vol. 7, no. 1-6, pp. 253-258, 1989.

NH Valerius, OI Stendahl, Hartwig JH, and TP Stossel, "Distribution of actin and myosin-binding protein in neutrophils during chemotaxis and phagocytosis," Adv. Exp. Med. Biol.1,vol. 41, p. 19-28., 1982.

J. Wie, LM Shaw, and AM Mercurio, "Integrin signaling in leukocytes: lessons from the ?6?1 integrin," J. Leukoc. Biol., Vol. 61, pp. 397-407.


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