Low Level Laser Irradiation Effects on Proliferation and Apoptosis in Bone Marrow Mesenchymal Stem Cells

Somayeh Delavarifar, Zahra Razi, Alireza Mehdizadeh, Mahin Salmannejad, Mahsa Sani, Mona Latifi, Seyed Mojtaba Hosseini

Abstract


Low level laser irradiationis an effective method in treating some diseases and it could improve regeneration and wound healing. Some studies have reported that low level laser irradiation could enhance proliferation by increasing ATP level in the cells mitochondria.Mesenchymal stem cells (MSCs) are a kind of stem cells which are capable of differentiating to other cells. Nowadays, MSCs are an appropriate option for regenerative medicine. In this study we want to investigate the optimum protocol for using low level laser irradiation to improve proliferation and make less apoptosis in Mesenchymal stem cells. The Mesenchymal stem cells were isolated from femur and tibia of a male rat and cultured in culture media. The isolated cells were differentiated to adipocyte and osteocyte to confirm their multi potency. The cells were irradiated with different exposure protocols of 808 nm diode laser. After exposure the stem cells markers (CD90, CD44 and CD45), doubling time, colony forming frequency and Caspase 3 activity (for apoptosis evaluation) were assessed.Both 640 mW (8th group) and 830 mW (14th group) have best result in colony forming and doubling time; however the 8th group had the least Caspase 3 activity. The patterns of stem cells markers expression have not changed after laser irradiation.The protocol used in 8th group could improve Mesenchymal stem cells proliferation more than the rest protocols and also it might make the apoptosis less than the other group. This irradiation protocol could be useful in regenerative medicine due to its effects on Mesenchymal stem cells.


Keywords


diode laser; bone marrow stem cell; proliferation; Apoptosis.

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References


S. Bouvet-Gerbettaz, E. Merigo, J. Rocca, G. Carle, N. Rochet, "Effects of lowlevel laser therapy on proliferation and differentiation of murine bone marrow cells into osteoblasts and osteoclasts", Lasers Surg Med, 41, 291-297,(2009).

G. Xuejuan, X. Da, "Molecular mechanisms of cell proliferation induced by low-power laser irradiation", J Biomedical Sci, 16, 4, (2009).

T. Yaakobi, L. Maltz, U. Oron, "Promotion of bone repair in the cortical bone of the tibia in rats by low energy laser (He-Ne) irradiation", Calcif Tissue Int, 59,297-300, (1996).

A. Ehud, R. Mordechai, B. Michael, S. Arie, S. Michal, "Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve" Brain Res, 476, 205-212, (1998).

M. Conlan, J. Rapley, C. Cobb, "Biostimulation of wound healing by low-energy laser irradiation", J Clin Periodontol, 23, 492-496, (1996).

S. Passarella, E. Casamassima, S. Molinari, D. Pastore, E. Quagliariello, I.M. Catalano, A. Cingolani, "Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium-neon laser", FEBS Lett, 175, 95-99, (1984).

W. Hu, J. Wang, C. Yu, C. Lan, G. Chen, H. Yu, "Helium-Neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria", J Invest Dermatol, 127, 20482057, (2007).

X. Gao, D. Xing, "Molecular mechanisms of cell proliferation induced by low-power laser irradiation", J Biomed Sci, 16, (2009).

T. Karu, "Mitochondrial signaling in mammalian cells activated by red and near-IR radiation", Photochem Photobiol, 84, 1091-1099, (2008).

M. Kreisler, A. Christoffers, H. Al-Haj, B. Willershausen, B. d'Hoedt, "Low-level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts", Lasers Surg Med, 30, 365-369, (2002).

J. De Castro, A. Pinheiro, C. Werneck, C. Soares, "The effect of laser therapy on the proliferation of oral KB carcinoma cells: an in vitro study", Photomed Laser Surg, 23, 586-589, (2005).

C. Werneck, A. Pinheiro, M. Pacheco, C. Soares, J. de Castro, "Laser light is capable of inducing proliferation of carcinoma cells in culture: a spectroscopic in vitro study", Photomed Laser Surg, 23, 300-303, (2005).

P. Moore, T. Ridgway, R. Higbee, E. Howard, M. Lucroy, "Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro", Lasers Surg Med, 36, 8-12, (2005).

P. Eduardo Fde, D. Bueno, P. De Freitas, M. Marques, M. Passos-Bueno, P. Eduardo Cde, a. et, "Stem cell proliferation under low intensity laser irradiation: a preliminary study", Lasers Surg Med, 40, 433-438, (2008).

R. Freshney, in: A.r.l. inc (Ed.) Culture of animal cells: a manual of basic technique, willey-blackwell, 1994.

k. Horvat-karajz, z. balogh, v. kovacs, a. drrernat, l. sreter, f. uher, "in vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells", Lasers surg med, 41, 463-469, (2009).

W. Wagner, A. Ho, "Mesenchymal stem cell preparations comparing apples and oranges", Stem Cell Rev, 3, 239248, (2007).

A.I. Caplan, "Mesenchymal stem cells", J Orthop Res, 9,641650, (1991).

M. Dominici, K. Le Blanc, I. Mueller, I. Slaper-Cortenbach, F. Marini, D. Krause, R. Deans, A. Keating, D. Prockop, E. Horwitz, "Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement", Cytotherapy, 8, 315317, (2006).

N. Mirsky, Y. Krispel, Y. Shoshany, L. Maltz, U. Oron, "promotion of angiogenesis by low energy laser irradiation", Antioxid redox signal 4,785-790, (2002).

H. Tuby, L. Maltz, U. Oron, "Low-level laser irradiation (LLLT) promotes proliferation of mesenchymal and cardiac stem cells in culture", Lasers Surg Med, 39, 373-378, (2007).

K. Bymes, x. Wu, R. Waynant, I. Ilev, J. Anders, "Low-power laser irradiation alters gene expression of olfactory ensheathing cells in vitro", Lasers Surg Med, 37, 161-171, (2005).

u. Oron, s. llic, L. taboada, J. Streeter, "Ga-As (808 nm) laser irradiation enhances ATP production in human neuronal cells in culture", Photomed Laser Surg, 25, 180-182, (2007).

Z.W. Kang XQ, Song TS, et al, "Isolation, culture and morphology observation of rat bone marrow mesenchymal stem cells", Xi an Jiaotong Daxue Xuebao: Yixue Ban, 24, 518-519, (2005).

D. Phinney, G. Kopen, R. Isaacson, D. Prockop, "Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and ifferentiation", J Cell Biochem, 72, 570585, (1999).

H. Castro-Malaspina, R. Gay, G. Resnick, N. Kapoor, P. Meyers, D. Chiarieri, S. McKenzie, H. Broxmeyer, M. Moore, Characterization of human bone marrow fibroblast colony- forming cells (CFU-F) and their progeny, Blood, 56, 289 301, (1980).

M. Khalid, AlGhamdi, A. Kumar, N.A. Moussa, "Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells", Lasers Med Sci, 27, 237-249, (2012).

D. Taniguchi, P. Dai, T. Hojo, Y. Yamaoka, T. Kubo, T. Takamatsu, "Low- energy laser irradiation promotes synovial fibroblast prolifration bu modulating p15 subcellular localization", Lasers Surg Med, 41, 232-239, (2009).

M. Xu, T. Deng, F. Mo, B. Deng, W. Lam, P. Deng, X. Zhang, S. Liu, "Low-intensity pulsed laser irradiaton affects RANKL and OPG mRNA expression in rat calvarial cells", Photomed Laser Surg, 27, 309-315, (2009).

H. Henry, "Is all nuclear radiation harmful?", Health Phys, 43, 767-769, (1982).

M. Ristow, K. Zarse, "How increased oxidative stress promotes longevity and metabolic health: The concept of mitochondrial hormesis (mitohormesis)", Experimental Gerontology, 45, 410-418, (2010).

M. Kohno, J. Pouyssegur, "Targeting the ERK signaling pathway in cancer therapy", Ann Med, 38, 200211, (2006).

L. Cantley, "The phosphoinositide 3-kinase pathway", Science, 296,16551657, (2002).

P. Wilden, Y. Agazie, R. Kaufman, S. Halenda, "ATP-stimulated smooth muscle cell proliferation requires independent ERK and PI3K signaling pathways", Am J Physiol, 275,12091215, (1998).

G. Shefer, I. Barash, U. Oron, O. Halevy, "Low-energy laser irradiation enhances de novo protein synthesis via its effects on translation-regulatory proteins in skeletal muscle myoblasts", Biochim Biophys Acta, 1593, 131-139, (2003).

S. Wang, N. Omori, F. Li, G. Jin, W. Zhang, Y. Hamakawa, K. Sato, I. Nagano, M. Shoji, K. Abe, "Potentiation of AKt and suppression of caspase-9 activations by electroacupuncture after transient middle cerebral artery occlusion in rats", Neurosci Lett, 331, 115-118, (2002).

J. Weise, R. Sandau, S. Schwarting, O. Crome, A. Wrede, W. Schulz-Schaeffer, I. Zerr, M. Bahr, "Deletion of cellular prion protein results in reduced AKt activation, enhanced postischemic caspase-3 activation and exacerbation of ischemic brain injury", Stroke, 37, 1296-1300, (2006).

X. Wang, K. McCullough, T. Franke, N. Holbrook, "Epidermal growth factor receptor dependant AKt avtivation by oxidative stress enhances cell survival", J Biol Chem, 275, 14624-14631, (2000).


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