Pre- and Postharvest Microbial and Cytogenetic Assessment of Irradiated Philippine Aglibut Sweet Tamarind (AST) Fruit (Tamarindus Indica)

G.E. Santos, J.V. Bagunu, D.E. Gracilla, E.V. Totaan, V.T. Bagunu

Abstract


The pre-and post-harvest practices of Aglibut Sweet Tamarind farmers were determined and correlated to the microbiological and cytogenetic quality of irradiated and non-irradiated AST fruits (Tamarindus indica) using Total Bacterial Count (TBC), Yeast and Mold (YM) test, and C-metaphase chromosome analysis. Some of the practices relative to possible fruit contamination are hand picking, unsanitized container and contact to the soil during harvesting. The initial microbial count is possibly associated with unacceptable farmers practices during pre-harvest and harvesting process. Microbial analyses established that T3 (irradiated AST fruit at 5kGy) is the best irradiation dose to extend the shelf life of fresh tamarind fruit up to 20 days followed by T2 (irradiated AST fruit at 3kGy) that has also the capability to extend the shelf life up to 15 days. On the other hand, T1 (Irradiated AST fruit at 1 kGy) and control are comparable with each other but cannot prolong the shelf-life of tamarind fruit as compared to T3 (irradiated AST fruit at 5kGy and T2 (irradiated AST fruit at 3kGy). Irradiated AST fruit extract did not cause chromosomal aberrations in white mice in all treatments. Hence, the irradiated Sweet Tamarind Fruit at 5kGy is found to be microbiologically and cytogenetically safe.


Keywords


chromosomal aberration; Cytological profile; clastogenic; Irradiation; Microbial profile.

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