Pre- and Postharvest Microbial and Cytogenetic Assessment of Irradiated Philippine Aglibut Sweet Tamarind (AST) Fruit (Tamarindus Indica)

G.E. Santos, J.V. Bagunu, D.E. Gracilla, E.V. Totaan, V.T. Bagunu


The pre-and post-harvest practices of Aglibut Sweet Tamarind farmers were determined and correlated to the microbiological and cytogenetic quality of irradiated and non-irradiated AST fruits (Tamarindus indica) using Total Bacterial Count (TBC), Yeast and Mold (YM) test, and C-metaphase chromosome analysis. Some of the practices relative to possible fruit contamination are hand picking, unsanitized container and contact to the soil during harvesting. The initial microbial count is possibly associated with unacceptable farmers practices during pre-harvest and harvesting process. Microbial analyses established that T3 (irradiated AST fruit at 5kGy) is the best irradiation dose to extend the shelf life of fresh tamarind fruit up to 20 days followed by T2 (irradiated AST fruit at 3kGy) that has also the capability to extend the shelf life up to 15 days. On the other hand, T1 (Irradiated AST fruit at 1 kGy) and control are comparable with each other but cannot prolong the shelf-life of tamarind fruit as compared to T3 (irradiated AST fruit at 5kGy and T2 (irradiated AST fruit at 3kGy). Irradiated AST fruit extract did not cause chromosomal aberrations in white mice in all treatments. Hence, the irradiated Sweet Tamarind Fruit at 5kGy is found to be microbiologically and cytogenetically safe.


chromosomal aberration; Cytological profile; clastogenic; Irradiation; Microbial profile.

Full Text:



D. S Murthy. and T. M. Ganjanah. Marketing and Post-Harvest in Fruits. Availability and Implication. Indian Journal of Agricultural Business. 16:24. 2009.

Berger, et al. Gamma Radiation against toxigenic fungi in food, medical and aromatic herbs. Science Against Microbial Pathogens. 4:29-35.2010.

S. Aquino, S. Gamma Radiation against toxigenic fungi in food, medical and aromatic herbs. Science Against Microbial Pathogens. 6:2. 2011.

D. Endoh, D. Micronucleus test in mice fed on an Irradiated Diet. Jph. Jvet. Res., 37:41-47. 1989.

J. Schubert. Mutagenicity and Cytotoxicity of Irradiated Food and Component. Bul. Org Mon, Sante. 873:94. 1969.

D.E. Gracilla, and J.V. Bagunu. Radiation Cytogenetic studies on Philippine Ashitaba (Gynura nepalensis D.C.).International Journal of Sciences: Basic and Applied Research. 17:1. 2014.

L.L. Solomon, Personal Communication, December 19, 2014- 6pm, Nuclear Services Division. Philippine Nuclear Research Institute, Commonwealth Avenue, Diliman, Quezon City Philippines.

L.L Cavinta,., et al. Laboratory Manual in Food and Water Microbiology: Department of Medical Microbiology, College of Public Health University of the Philippines Manila. 2009.

F.S. Gregorio,. Experiment No.2 Experimental Procedure and Theory in Cytogenetic Laboratory. Philippine Nuclear Research Institute. 2009.

. Statsoft Inc. (1999). Statistica 99th Edition. USA.

Ministry of Health (1995). Microbiological Reference Criteria for Food, Food Administration section of the

Ministry of Health. Institute of Environmental Science and Research.

B. Kalyani, B. and K. Manjula, K. Food Irradiation - Technology and Application, International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706,3:4:549-555(2014

IAEA (International Atomic Energy Agency), (2009). Irradiation to ensure the safety and quality of prepared meals, Vienna, Austria,. 375.

J. Farkas,. Charged particle and photon interactions with matter, In: Mozumder, A. & Hatano, Y. (eds): Food Irradiation, Marcel Dekker, New York, 785812. 2004.

K. Tomlins, (2008). Food safety and quality management. Food Africa.

BAIF . Fruits for the future: Tamarind messages programme and technologies on sustainability Newsletter, 2002.

S.S. Bhattacharya, and R. K. Mukherjee.. Functional and nutritional properties of (Tamarindus indica) kernel protein. Food chemistry, 49: 1-9. 1994.


  • There are currently no refbacks.





About IJSBAR | Privacy PolicyTerms & Conditions | Contact Us | DisclaimerFAQs 

IJSBAR is published by (GSSRR).