A novel Approach for Detection of Sindbis Viral RNA with QPCR

Nahla Mohamed, Yazan Odeh


SINV was approved as a causative agent of pogosta disease. The seroprevalence of SINV antibodies for the Finnish population is around 2%, considering the prevalence varies between different regions of Finland. While the seroprevalence of SINV antibodies in Sweden highest in central parts of the country. The annual incidence rate in endemic regions of affected countries ranges from 2.7/100,000 in Finland and 2.9/100,000 in Sweden to 18/100,000 in Northern Karelia. This is the most widely distributed of all known arboviruses, affecting all age groups. This study describes the design and evaluation of a rapid and robust quantitative PCR assay able to detect a wide range of different SINV. Primers with the potential to detect all SINV were designed from conserved regions of all different strains of sindbis virus sequences, as identified from multiple alignments. By using SYBR-green-based quantitative real-time PCR (QPCR) protocols, this QPCR assay is able to detect 50-100 target molecules ofsynthetic DNA and less than 100 copies of viral RNA of different SINV. SINV RNA was also detected in clinical samples of patients with SINV has been linked toPogosta diseaseinFinland. Ockelbo is a disease in Sweden and Karelian fever in Russia. The real-time RT-PCR assay is specific and sensitive for detection of SINV and can used for screening SINV in wildlife. This current assay provides a powerful tool for research and diagnostic laboratories where different strains of SINV are circulating worldwide and may be useful in surveys with the purpose of finding new SINV in man and other species.


Pogosta disease; SINV; QPCR; mosquito species; bats; ticks; humans; viral RNA.

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