Performance of the First Generation Crossbred Common Carp Bearing Growth and Disease KHV Resistance Related Molecular Markers

. Rina, Odang Carman, . Alimuddin, Komar Sumantadinata, Muhammad Zairin Jr


Genetic improvement by using marker assisted selection (MAS) can save time and facilities. Common carp (Cyprinuscarpio L) in Indonesia has been reported to have DNA marker Cca-08 which related to fast growth, and Cyca-DAB1*05 associated with resistance to infection of koi herpesvirus (KHV). This research was conducted to find out the performance of offspring ten crossbreeding common carp by using the two DNA markers. Broodstocks were collected from Wanayasa Freshwater Fish Seed Development Center and Sukabumi Main Center of Freshwater Aquaculture Development. The existence of Cca-08 marker was analyzed by using microsatellite, while Cyca-DAB1*05 by PCR with specific primer. The results showed that most of broodstock from Wanayasa bearing Cca-08 marker (92.4%), while from Sukabumi was Cyca-DAB1*05 marker (94.4%). Percentage of broodstocks bearing the two markers was 9.4% from Wanayasa, and 38.9% from Sukabumi. Inheritance pattern of the markers in progenies was different among crossbred. All crosses showed 65% or more progenies bear the Cca-08, while the Cyca-DAB1*05 was present with the highest percentage in crossbreeding between female homozygote Cca-08 with Cyca-DAB1*05 and male without Cca-08 and Cyca-DAB1*05

(70%). The best performance of growth and survival rate was obtained in crossbreeding between female heterozygote Cca-08 without Cyca-DAB1*05 and male heterozygote Cca-08 with Cyca-DAB1*05, and this broodstock could be used to produce superior common carp seed.


growth; CyprinuscarpioL; resistance to KHV; molecular marker.

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Vandeputte M, Kocour M, Mauger S, Mathilde DN, Daphne DG, Rodina M, Gela D, Vallod D, Chevassus B, Linhart O. Heritability estimates for growth-related traits using microsatellite parentage assignment in juvenile common crap (CyprinuscarpioL). Aquaculture, 235:223-236, 2004.

Moria SB, Permana IGN, Susanto B. Karakteristikgenetikindukrajungan (Portunuspelagius) daribeberapaperairanmelaluianalisa RFLP mt-DNA.JurnalPenelitianPerikanan Indonesia, 11(5):57-62,2005.

Thai BT, Burridge CP, Austin CM. . Genetic diversity of common carp (CyprinuscarpioL) in Vietnam using four microsatellite loci. Aquaculture, 269:174-186, 2007

Nugroho E. Pemuliaanikan mas: PelajaranberhargadariHongaria. Media Akuakultur 2 (1): 151-155, 2007.

Tave D. Selective breeding programs for medium-size fish farms. FAO Fisheries Technical Paper, Rome, 1995, 352

Liu ZJ, Cordes JF. DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238:1-37, 2004.

Hayes B, Andersen . Modern biotechnology and aquaculture. In: Gjederm T. (ed) Selection in breeding programs in aquaculture. AKVAFORSK. Institute of Aquaculture Research AS, Norway. Springer. Netherlands, 2005, pp 301-318.

Araneda C, Neira R, Itturra P. Identification of dominant SCAR marker associated with color traits in coho salmon (Oncorhynchuskisutch). Aquaculture 247: 67-73, 2005.

Sun X, Liang L. A genetic linkage map of common carp (Cyprinuscarpio L) and mapping of a locus associated with cold tolerance. Aquaculture, 238: 165-172, 2004.

Rodriguez MF, La Patra S, William S, Famula T, May B. Genetic markers associated with resistance to infectious hematopoietic necrosis in rainbow and steelhead trout (Oncorhynchusmykiss) backcrosses. Aquaculture, 241: 93-115, 2004.

Sumantri C, Farajallah A, Fauzi U, Salamena J.F. KeragamanGenetik DNA MikrosatelitdanHubungannyadengan Performa BobotpadaDombaLokal. Media Peternakan, 31 (1): 1-13, 2008.

Vandeputte M, Rossignol MN, Pincent C. From theory to practice: Empirical evaluation of assignment power of marker sets for pedigree analysis in fish breeding. Aquaculture, 314: 80-86, 2011

Maskur. Analisisketerkaitanantaralokus Cca-08 denganpertumbuhandankelangsunganhidupbenihikan mas Cangkringan (Cyprinuscarpio). Thesis. Program StudiIlmuPerairan. SekolahPascasarjanaInstitutPertanian Bogor.2010

Alimuddin, Mubinun, Santika A, Carman O, Faizal I, Sumantadinata K. Identification of the Majalaya common carp strain resistance to KHV infection using Cyca-DAB1*05-DAB1*05 allele as a marker. Indonesia Aquaculture Journal, 6 (2): 157-163, 2011.

Rakus KL, Wiegertjes G.F., Siwicki, A.K., Lepa A., Adamek M., Irnazarow I. . Resistance of common carp (Cyprinuscarpio L.) to Cyprinid herpesvirus-3 is influenced by major histocompatibility (MH) class II B gene polymorphism inMajor histocompability (MH) polymorphism of common carp. Link with diseases resistance.Wageningen University. Netherlands,2008

Aliah RS. Studies on genetic change in common- and oriental carp (Cyprinuscarpio), using microsatellite DNA markers.Thesis.Tohoku University.128 pp, 2000.

Chistiakov DA, Hellemans B, Volckaert FAM. Microsatellite and their genomic distribution, evolution, function and applications: A review with special reference to fish genetics. Aquaculture, 225:1-29, 2006.

Huisman EA.Food convertion efficiencies at maintenance and production levels of carp and rainbowtrout.Aquaculture. 1989. 9:259-273, 1989

Okumus I, Ciftci Y.Fish Population Genetics and Molecular Markers: II-Molecular Markers and their applications in fisheries and aquaculture.Turkish Journal of Fisheries and Aquatic Sciences.3: 51-79, 2003.

Rakus KL, Irnazarov I, Forlenza M, Stet RJM, Savelkoul HFJ, Wiegertjes GF. Classical crosses of common carp (Cyprinuscarpio L.) show co-segregation of antibody response with major histocompatibility class II B genes in Majorhistocompability (MH) polymorphism of common carp.Link with diseases resistance.Wageningen University. Netherlands. 2008.


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